Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1340 and Psest_1341 are separated by 37 nucleotides Psest_1341 and Psest_1342 are separated by 60 nucleotides Psest_1342 and Psest_1343 are separated by 16 nucleotides
Psest_1340: Psest_1340 - rarD protein, at 1,450,691 to 1,451,620
_1340
Psest_1341: Psest_1341 - FOG: FHA domain, at 1,451,658 to 1,452,512
_1341
Psest_1342: Psest_1342 - Protein kinase domain., at 1,452,573 to 1,454,126
_1342
Psest_1343: Psest_1343 - Serine/threonine protein phosphatase, at 1,454,143 to 1,454,919
_1343
Position (kb)
1452
1453
1454
1455 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.953 kb on - strand, within Psest_1341 at 1451.953 kb on - strand, within Psest_1341 at 1452.058 kb on + strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.152 kb on - strand, within Psest_1341 at 1452.152 kb on - strand, within Psest_1341 at 1452.322 kb on + strand, within Psest_1341 at 1452.322 kb on + strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.355 kb on + strand, within Psest_1341 at 1452.356 kb on - strand, within Psest_1341 at 1452.356 kb on - strand, within Psest_1341 at 1452.358 kb on + strand, within Psest_1341 at 1452.359 kb on - strand, within Psest_1341 at 1452.359 kb on - strand, within Psest_1341 at 1452.521 kb on + strand at 1452.748 kb on + strand, within Psest_1342 at 1452.781 kb on + strand, within Psest_1342 at 1452.781 kb on + strand, within Psest_1342 at 1452.781 kb on + strand, within Psest_1342 at 1452.781 kb on + strand, within Psest_1342 at 1452.781 kb on + strand, within Psest_1342 at 1452.782 kb on - strand, within Psest_1342 at 1452.782 kb on - strand, within Psest_1342 at 1452.782 kb on - strand, within Psest_1342 at 1452.782 kb on - strand, within Psest_1342 at 1452.814 kb on + strand, within Psest_1342 at 1452.815 kb on - strand, within Psest_1342 at 1452.838 kb on + strand, within Psest_1342 at 1452.838 kb on + strand, within Psest_1342 at 1452.910 kb on + strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1452.914 kb on - strand, within Psest_1342 at 1453.072 kb on + strand, within Psest_1342 at 1453.072 kb on + strand, within Psest_1342 at 1453.072 kb on + strand, within Psest_1342 at 1453.073 kb on - strand, within Psest_1342 at 1453.073 kb on - strand, within Psest_1342 at 1453.091 kb on - strand, within Psest_1342 at 1453.162 kb on + strand, within Psest_1342 at 1453.163 kb on - strand, within Psest_1342 at 1453.178 kb on - strand, within Psest_1342 at 1453.178 kb on - strand, within Psest_1342 at 1453.423 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.552 kb on + strand, within Psest_1342 at 1453.553 kb on - strand, within Psest_1342 at 1453.553 kb on - strand, within Psest_1342 at 1453.553 kb on - strand, within Psest_1342 at 1453.606 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.861 kb on + strand, within Psest_1342 at 1453.862 kb on - strand, within Psest_1342 at 1453.862 kb on - strand, within Psest_1342 at 1453.877 kb on - strand, within Psest_1342 at 1454.004 kb on + strand at 1454.004 kb on + strand at 1454.005 kb on - strand at 1454.017 kb on - strand at 1454.126 kb on + strand at 1454.847 kb on + strand at 1454.847 kb on + strand at 1454.885 kb on + strand at 1455.034 kb on + strand at 1455.034 kb on + strand at 1455.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,451,952 + Psest_1341 0.34 +0.4 1,451,952 + Psest_1341 0.34 -1.7 1,451,952 + Psest_1341 0.34 -3.5 1,451,952 + Psest_1341 0.34 -0.7 1,451,953 - Psest_1341 0.35 -0.6 1,451,953 - Psest_1341 0.35 -0.4 1,452,058 + Psest_1341 0.47 -0.4 1,452,059 - Psest_1341 0.47 -1.4 1,452,059 - Psest_1341 0.47 -1.2 1,452,059 - Psest_1341 0.47 +0.0 1,452,059 - Psest_1341 0.47 -1.8 1,452,059 - Psest_1341 0.47 -2.2 1,452,059 - Psest_1341 0.47 -0.4 1,452,059 - Psest_1341 0.47 -1.2 1,452,059 - Psest_1341 0.47 -1.1 1,452,151 + Psest_1341 0.58 -0.1 1,452,151 + Psest_1341 0.58 +1.0 1,452,151 + Psest_1341 0.58 +1.2 1,452,152 - Psest_1341 0.58 -2.7 1,452,152 - Psest_1341 0.58 -3.0 1,452,322 + Psest_1341 0.78 -1.4 1,452,322 + Psest_1341 0.78 -0.5 1,452,323 - Psest_1341 0.78 -1.7 1,452,323 - Psest_1341 0.78 -1.9 1,452,323 - Psest_1341 0.78 +1.0 1,452,323 - Psest_1341 0.78 -1.4 1,452,323 - Psest_1341 0.78 -1.9 1,452,355 + Psest_1341 0.82 -0.6 1,452,356 - Psest_1341 0.82 -0.8 1,452,356 - Psest_1341 0.82 -1.9 1,452,358 + Psest_1341 0.82 +1.3 1,452,359 - Psest_1341 0.82 -1.2 1,452,359 - Psest_1341 0.82 +0.6 1,452,521 + -0.1 1,452,748 + Psest_1342 0.11 -0.5 1,452,781 + Psest_1342 0.13 +2.0 1,452,781 + Psest_1342 0.13 -0.3 1,452,781 + Psest_1342 0.13 -1.9 1,452,781 + Psest_1342 0.13 -0.6 1,452,781 + Psest_1342 0.13 +0.3 1,452,782 - Psest_1342 0.13 -2.2 1,452,782 - Psest_1342 0.13 -0.8 1,452,782 - Psest_1342 0.13 -2.2 1,452,782 - Psest_1342 0.13 -0.7 1,452,814 + Psest_1342 0.16 -2.3 1,452,815 - Psest_1342 0.16 +1.9 1,452,838 + Psest_1342 0.17 +0.6 1,452,838 + Psest_1342 0.17 -0.3 1,452,910 + Psest_1342 0.22 -0.9 1,452,914 - Psest_1342 0.22 -1.7 1,452,914 - Psest_1342 0.22 -1.4 1,452,914 - Psest_1342 0.22 -1.4 1,452,914 - Psest_1342 0.22 -2.9 1,452,914 - Psest_1342 0.22 +1.0 1,452,914 - Psest_1342 0.22 +0.4 1,453,072 + Psest_1342 0.32 -1.9 1,453,072 + Psest_1342 0.32 +0.7 1,453,072 + Psest_1342 0.32 +1.1 1,453,073 - Psest_1342 0.32 -1.6 1,453,073 - Psest_1342 0.32 -2.5 1,453,091 - Psest_1342 0.33 +0.9 1,453,162 + Psest_1342 0.38 -0.4 1,453,163 - Psest_1342 0.38 +0.4 1,453,178 - Psest_1342 0.39 -0.7 1,453,178 - Psest_1342 0.39 -0.6 1,453,423 + Psest_1342 0.55 -0.3 1,453,552 + Psest_1342 0.63 +0.0 1,453,552 + Psest_1342 0.63 +0.4 1,453,552 + Psest_1342 0.63 +1.1 1,453,552 + Psest_1342 0.63 +0.9 1,453,552 + Psest_1342 0.63 +0.0 1,453,552 + Psest_1342 0.63 +1.3 1,453,552 + Psest_1342 0.63 -0.1 1,453,553 - Psest_1342 0.63 -0.2 1,453,553 - Psest_1342 0.63 +0.1 1,453,553 - Psest_1342 0.63 -1.2 1,453,606 + Psest_1342 0.66 +0.6 1,453,861 + Psest_1342 0.83 -0.4 1,453,861 + Psest_1342 0.83 +0.4 1,453,861 + Psest_1342 0.83 +0.6 1,453,861 + Psest_1342 0.83 -1.7 1,453,861 + Psest_1342 0.83 -1.4 1,453,861 + Psest_1342 0.83 -2.6 1,453,861 + Psest_1342 0.83 +0.2 1,453,862 - Psest_1342 0.83 +1.2 1,453,862 - Psest_1342 0.83 -1.9 1,453,877 - Psest_1342 0.84 +0.2 1,454,004 + -2.0 1,454,004 + -1.0 1,454,005 - -0.8 1,454,017 - -0.2 1,454,126 + -0.3 1,454,847 + -0.4 1,454,847 + +0.5 1,454,885 + -0.4 1,455,034 + -1.2 1,455,034 + -0.2 1,455,049 - +0.0
Or see this region's nucleotide sequence