Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1272

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1269 and Psest_1270 are separated by 11 nucleotidesPsest_1270 and Psest_1271 are separated by 55 nucleotidesPsest_1271 and Psest_1272 overlap by 15 nucleotidesPsest_1272 and Psest_1273 overlap by 4 nucleotidesPsest_1273 and Psest_1274 are separated by 74 nucleotides Psest_1269: Psest_1269 - Predicted amidohydrolase, at 1,374,769 to 1,375,563 _1269 Psest_1270: Psest_1270 - hypothetical protein, at 1,375,575 to 1,375,766 _1270 Psest_1271: Psest_1271 - Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family, at 1,375,822 to 1,376,355 _1271 Psest_1272: Psest_1272 - Protein of Unknown function (DUF2784)., at 1,376,341 to 1,376,712 _1272 Psest_1273: Psest_1273 - Predicted membrane protein, at 1,376,709 to 1,377,344 _1273 Psest_1274: Psest_1274 - Endonuclease I, at 1,377,419 to 1,378,132 _1274 Position (kb) 1376 1377Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1375.351 kb on + strand, within Psest_1269at 1375.352 kb on - strand, within Psest_1269at 1375.352 kb on - strand, within Psest_1269at 1375.567 kb on + strandat 1375.568 kb on - strandat 1375.612 kb on + strand, within Psest_1270at 1375.612 kb on + strand, within Psest_1270at 1375.612 kb on + strand, within Psest_1270at 1375.612 kb on + strand, within Psest_1270at 1375.613 kb on - strand, within Psest_1270at 1375.613 kb on - strand, within Psest_1270at 1375.640 kb on - strand, within Psest_1270at 1375.760 kb on + strandat 1375.852 kb on + strandat 1375.852 kb on + strandat 1375.852 kb on + strandat 1375.852 kb on + strandat 1376.122 kb on - strand, within Psest_1271at 1376.122 kb on - strand, within Psest_1271at 1376.280 kb on + strand, within Psest_1271at 1376.280 kb on + strand, within Psest_1271at 1376.280 kb on + strand, within Psest_1271at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.381 kb on + strand, within Psest_1272at 1376.385 kb on + strand, within Psest_1272at 1376.385 kb on + strand, within Psest_1272at 1376.385 kb on + strand, within Psest_1272at 1376.385 kb on + strand, within Psest_1272at 1376.423 kb on + strand, within Psest_1272at 1376.423 kb on + strand, within Psest_1272at 1376.423 kb on + strand, within Psest_1272at 1376.423 kb on + strand, within Psest_1272at 1376.423 kb on + strand, within Psest_1272at 1376.424 kb on - strand, within Psest_1272at 1376.424 kb on - strand, within Psest_1272at 1376.424 kb on - strand, within Psest_1272at 1376.471 kb on + strand, within Psest_1272at 1376.605 kb on + strand, within Psest_1272at 1376.605 kb on + strand, within Psest_1272at 1376.605 kb on + strand, within Psest_1272at 1376.606 kb on - strand, within Psest_1272at 1376.705 kb on + strandat 1376.706 kb on - strandat 1376.914 kb on + strand, within Psest_1273at 1376.915 kb on - strand, within Psest_1273at 1377.055 kb on + strand, within Psest_1273at 1377.056 kb on - strand, within Psest_1273at 1377.056 kb on - strand, within Psest_1273at 1377.062 kb on + strand, within Psest_1273at 1377.062 kb on + strand, within Psest_1273at 1377.115 kb on + strand, within Psest_1273at 1377.116 kb on - strand, within Psest_1273at 1377.119 kb on + strand, within Psest_1273at 1377.119 kb on + strand, within Psest_1273at 1377.120 kb on - strand, within Psest_1273at 1377.120 kb on - strand, within Psest_1273at 1377.120 kb on - strand, within Psest_1273at 1377.120 kb on - strand, within Psest_1273at 1377.121 kb on + strand, within Psest_1273at 1377.121 kb on + strand, within Psest_1273at 1377.121 kb on + strand, within Psest_1273at 1377.122 kb on - strand, within Psest_1273at 1377.166 kb on - strand, within Psest_1273at 1377.169 kb on + strand, within Psest_1273at 1377.169 kb on + strand, within Psest_1273at 1377.169 kb on + strand, within Psest_1273at 1377.170 kb on - strand, within Psest_1273at 1377.170 kb on - strand, within Psest_1273at 1377.170 kb on - strand, within Psest_1273at 1377.204 kb on - strand, within Psest_1273at 1377.204 kb on - strand, within Psest_1273at 1377.204 kb on - strand, within Psest_1273at 1377.204 kb on - strand, within Psest_1273at 1377.204 kb on - strand, within Psest_1273at 1377.263 kb on + strand, within Psest_1273at 1377.269 kb on - strand, within Psest_1273at 1377.344 kb on + strandat 1377.345 kb on - strandat 1377.373 kb on + strandat 1377.373 kb on + strandat 1377.396 kb on - strandat 1377.497 kb on + strand, within Psest_1274at 1377.498 kb on - strand, within Psest_1274at 1377.568 kb on - strand, within Psest_1274at 1377.568 kb on - strand, within Psest_1274at 1377.568 kb on - strand, within Psest_1274at 1377.603 kb on + strand, within Psest_1274at 1377.618 kb on + strand, within Psest_1274at 1377.618 kb on + strand, within Psest_1274at 1377.618 kb on + strand, within Psest_1274at 1377.618 kb on + strand, within Psest_1274at 1377.618 kb on + strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.619 kb on - strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.624 kb on + strand, within Psest_1274at 1377.625 kb on - strand, within Psest_1274at 1377.651 kb on + strand, within Psest_1274at 1377.652 kb on - strand, within Psest_1274

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,375,351 + Psest_1269 0.73 +1.1
1,375,352 - Psest_1269 0.73 -0.0
1,375,352 - Psest_1269 0.73 +0.4
1,375,567 + +0.5
1,375,568 - -1.9
1,375,612 + Psest_1270 0.19 -0.4
1,375,612 + Psest_1270 0.19 +0.7
1,375,612 + Psest_1270 0.19 -2.9
1,375,612 + Psest_1270 0.19 +1.2
1,375,613 - Psest_1270 0.20 -0.0
1,375,613 - Psest_1270 0.20 -0.4
1,375,640 - Psest_1270 0.34 +0.5
1,375,760 + +1.4
1,375,852 + +2.1
1,375,852 + -0.1
1,375,852 + -2.1
1,375,852 + +1.1
1,376,122 - Psest_1271 0.56 +1.5
1,376,122 - Psest_1271 0.56 +0.3
1,376,280 + Psest_1271 0.86 +0.1
1,376,280 + Psest_1271 0.86 +1.4
1,376,280 + Psest_1271 0.86 +0.4
1,376,381 + Psest_1272 0.11 +0.4
1,376,381 + Psest_1272 0.11 -0.7
1,376,381 + Psest_1272 0.11 +0.6
1,376,381 + Psest_1272 0.11 +2.7
1,376,381 + Psest_1272 0.11 -0.1
1,376,381 + Psest_1272 0.11 +1.0
1,376,381 + Psest_1272 0.11 -1.8
1,376,381 + Psest_1272 0.11 +0.9
1,376,381 + Psest_1272 0.11 +1.4
1,376,381 + Psest_1272 0.11 +0.4
1,376,381 + Psest_1272 0.11 -0.2
1,376,381 + Psest_1272 0.11 -0.4
1,376,381 + Psest_1272 0.11 +1.5
1,376,381 + Psest_1272 0.11 -0.6
1,376,381 + Psest_1272 0.11 -1.5
1,376,381 + Psest_1272 0.11 -1.0
1,376,381 + Psest_1272 0.11 +0.9
1,376,385 + Psest_1272 0.12 +1.2
1,376,385 + Psest_1272 0.12 +2.9
1,376,385 + Psest_1272 0.12 -0.4
1,376,385 + Psest_1272 0.12 +1.0
1,376,423 + Psest_1272 0.22 -0.2
1,376,423 + Psest_1272 0.22 +0.2
1,376,423 + Psest_1272 0.22 -0.4
1,376,423 + Psest_1272 0.22 +0.4
1,376,423 + Psest_1272 0.22 +0.4
1,376,424 - Psest_1272 0.22 +0.8
1,376,424 - Psest_1272 0.22 +1.7
1,376,424 - Psest_1272 0.22 -0.3
1,376,471 + Psest_1272 0.35 -0.3
1,376,605 + Psest_1272 0.71 -0.7
1,376,605 + Psest_1272 0.71 +1.0
1,376,605 + Psest_1272 0.71 +0.2
1,376,606 - Psest_1272 0.71 +0.5
1,376,705 + +2.1
1,376,706 - -1.5
1,376,914 + Psest_1273 0.32 -0.6
1,376,915 - Psest_1273 0.32 -0.9
1,377,055 + Psest_1273 0.54 +0.1
1,377,056 - Psest_1273 0.55 +0.8
1,377,056 - Psest_1273 0.55 +0.5
1,377,062 + Psest_1273 0.56 +0.9
1,377,062 + Psest_1273 0.56 -0.8
1,377,115 + Psest_1273 0.64 +0.4
1,377,116 - Psest_1273 0.64 +0.1
1,377,119 + Psest_1273 0.64 +0.7
1,377,119 + Psest_1273 0.64 +0.5
1,377,120 - Psest_1273 0.65 +1.3
1,377,120 - Psest_1273 0.65 +0.4
1,377,120 - Psest_1273 0.65 +0.7
1,377,120 - Psest_1273 0.65 -1.3
1,377,121 + Psest_1273 0.65 -1.5
1,377,121 + Psest_1273 0.65 -1.3
1,377,121 + Psest_1273 0.65 +1.1
1,377,122 - Psest_1273 0.65 +0.5
1,377,166 - Psest_1273 0.72 -0.3
1,377,169 + Psest_1273 0.72 -1.3
1,377,169 + Psest_1273 0.72 +1.6
1,377,169 + Psest_1273 0.72 +0.7
1,377,170 - Psest_1273 0.72 -2.6
1,377,170 - Psest_1273 0.72 -1.4
1,377,170 - Psest_1273 0.72 -0.5
1,377,204 - Psest_1273 0.78 -0.5
1,377,204 - Psest_1273 0.78 +0.7
1,377,204 - Psest_1273 0.78 +0.6
1,377,204 - Psest_1273 0.78 +0.9
1,377,204 - Psest_1273 0.78 +1.5
1,377,263 + Psest_1273 0.87 -0.0
1,377,269 - Psest_1273 0.88 -0.1
1,377,344 + +0.8
1,377,345 - +2.7
1,377,373 + +1.7
1,377,373 + -2.7
1,377,396 - +0.0
1,377,497 + Psest_1274 0.11 +1.4
1,377,498 - Psest_1274 0.11 +0.6
1,377,568 - Psest_1274 0.21 -0.7
1,377,568 - Psest_1274 0.21 -2.2
1,377,568 - Psest_1274 0.21 -1.5
1,377,603 + Psest_1274 0.26 -0.9
1,377,618 + Psest_1274 0.28 +0.4
1,377,618 + Psest_1274 0.28 -1.0
1,377,618 + Psest_1274 0.28 +1.4
1,377,618 + Psest_1274 0.28 +0.6
1,377,618 + Psest_1274 0.28 -2.0
1,377,619 - Psest_1274 0.28 +1.3
1,377,619 - Psest_1274 0.28 +1.1
1,377,619 - Psest_1274 0.28 +0.1
1,377,619 - Psest_1274 0.28 -1.1
1,377,619 - Psest_1274 0.28 +0.7
1,377,619 - Psest_1274 0.28 +1.2
1,377,619 - Psest_1274 0.28 +0.7
1,377,624 + Psest_1274 0.29 +0.7
1,377,624 + Psest_1274 0.29 -0.7
1,377,624 + Psest_1274 0.29 +0.4
1,377,624 + Psest_1274 0.29 +1.1
1,377,624 + Psest_1274 0.29 +0.8
1,377,624 + Psest_1274 0.29 +1.2
1,377,624 + Psest_1274 0.29 +2.0
1,377,624 + Psest_1274 0.29 +0.7
1,377,624 + Psest_1274 0.29 +1.9
1,377,624 + Psest_1274 0.29 +0.1
1,377,624 + Psest_1274 0.29 -0.7
1,377,624 + Psest_1274 0.29 +0.5
1,377,624 + Psest_1274 0.29 -0.3
1,377,625 - Psest_1274 0.29 +1.6
1,377,651 + Psest_1274 0.32 +0.2
1,377,652 - Psest_1274 0.33 +0.7

Or see this region's nucleotide sequence