Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1181 and Psest_1182 are separated by 252 nucleotides Psest_1182 and Psest_1183 are separated by 5 nucleotides Psest_1183 and Psest_1184 are separated by 16 nucleotides
Psest_1181: Psest_1181 - Glyoxylate carboligase (EC 4.1.1.47) (from data) , at 1,283,252 to 1,285,033
_1181
Psest_1182: Psest_1182 - Transcriptional regulator, at 1,285,286 to 1,286,194
_1182
Psest_1183: Psest_1183 - hydroxyisourate hydrolase, at 1,286,200 to 1,286,610
_1183
Psest_1184: Psest_1184 - Uncharacterized protein, possibly involved in utilization of glycolate and propanediol, at 1,286,627 to 1,287,166
_1184
Position (kb)
1285
1286
1287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.390 kb on + strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.391 kb on - strand, within Psest_1181 at 1284.483 kb on + strand, within Psest_1181 at 1284.484 kb on - strand, within Psest_1181 at 1284.501 kb on + strand, within Psest_1181 at 1284.703 kb on + strand, within Psest_1181 at 1284.704 kb on - strand, within Psest_1181 at 1284.704 kb on - strand, within Psest_1181 at 1284.706 kb on - strand, within Psest_1181 at 1284.852 kb on + strand, within Psest_1181 at 1284.933 kb on + strand at 1285.163 kb on + strand at 1285.171 kb on + strand at 1285.171 kb on + strand at 1285.171 kb on + strand at 1285.172 kb on - strand at 1285.207 kb on - strand at 1285.272 kb on - strand at 1285.479 kb on - strand, within Psest_1182 at 1285.604 kb on - strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1286.067 kb on - strand, within Psest_1182 at 1286.067 kb on - strand, within Psest_1182 at 1286.081 kb on + strand, within Psest_1182 at 1286.115 kb on - strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.361 kb on - strand, within Psest_1183 at 1286.364 kb on + strand, within Psest_1183 at 1286.365 kb on - strand, within Psest_1183 at 1286.365 kb on - strand, within Psest_1183 at 1286.599 kb on + strand at 1286.599 kb on + strand at 1286.600 kb on - strand at 1286.600 kb on - strand at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,284,390 + Psest_1181 0.64 -0.4 1,284,390 + Psest_1181 0.64 -0.6 1,284,390 + Psest_1181 0.64 +0.2 1,284,390 + Psest_1181 0.64 -1.3 1,284,390 + Psest_1181 0.64 -0.4 1,284,390 + Psest_1181 0.64 -1.8 1,284,390 + Psest_1181 0.64 +1.9 1,284,390 + Psest_1181 0.64 +2.1 1,284,390 + Psest_1181 0.64 -1.0 1,284,390 + Psest_1181 0.64 -1.2 1,284,390 + Psest_1181 0.64 -1.1 1,284,390 + Psest_1181 0.64 -1.2 1,284,390 + Psest_1181 0.64 -2.1 1,284,391 - Psest_1181 0.64 +1.9 1,284,391 - Psest_1181 0.64 -0.0 1,284,391 - Psest_1181 0.64 -1.8 1,284,391 - Psest_1181 0.64 +0.6 1,284,391 - Psest_1181 0.64 -1.4 1,284,391 - Psest_1181 0.64 -1.8 1,284,391 - Psest_1181 0.64 -1.7 1,284,391 - Psest_1181 0.64 -1.1 1,284,391 - Psest_1181 0.64 +1.6 1,284,483 + Psest_1181 0.69 +1.6 1,284,484 - Psest_1181 0.69 +0.5 1,284,501 + Psest_1181 0.70 +1.6 1,284,703 + Psest_1181 0.81 +0.4 1,284,704 - Psest_1181 0.81 +1.6 1,284,704 - Psest_1181 0.81 -1.5 1,284,706 - Psest_1181 0.82 -0.1 1,284,852 + Psest_1181 0.90 -0.9 1,284,933 + -0.4 1,285,163 + +1.6 1,285,171 + -2.3 1,285,171 + -2.3 1,285,171 + -1.7 1,285,172 - +0.0 1,285,207 - -2.0 1,285,272 - -0.1 1,285,479 - Psest_1182 0.21 +0.3 1,285,604 - Psest_1182 0.35 -0.4 1,285,742 + Psest_1182 0.50 -0.4 1,285,742 + Psest_1182 0.50 +0.0 1,285,742 + Psest_1182 0.50 -0.7 1,285,742 + Psest_1182 0.50 -3.3 1,285,742 + Psest_1182 0.50 +0.9 1,285,743 - Psest_1182 0.50 -1.5 1,285,743 - Psest_1182 0.50 +0.6 1,285,743 - Psest_1182 0.50 -0.5 1,285,743 - Psest_1182 0.50 +0.8 1,285,743 - Psest_1182 0.50 -0.2 1,285,743 - Psest_1182 0.50 +0.1 1,286,067 - Psest_1182 0.86 -0.0 1,286,067 - Psest_1182 0.86 -0.8 1,286,081 + Psest_1182 0.87 -0.1 1,286,115 - +0.7 1,286,162 + +0.6 1,286,162 + +0.9 1,286,162 + -1.2 1,286,162 + -0.8 1,286,162 + -0.5 1,286,162 + +0.4 1,286,163 - -1.9 1,286,163 - +1.3 1,286,163 - +0.8 1,286,163 - +1.2 1,286,361 - Psest_1183 0.39 -2.5 1,286,364 + Psest_1183 0.40 +2.1 1,286,365 - Psest_1183 0.40 -1.0 1,286,365 - Psest_1183 0.40 -1.7 1,286,599 + -1.4 1,286,599 + -1.1 1,286,600 - -1.9 1,286,600 - -1.7 1,287,089 + Psest_1184 0.86 -0.6 1,287,089 + Psest_1184 0.86 +1.6 1,287,089 + Psest_1184 0.86 +1.9 1,287,089 + Psest_1184 0.86 +1.4 1,287,089 + Psest_1184 0.86 -1.9 1,287,089 + Psest_1184 0.86 +2.0 1,287,089 + Psest_1184 0.86 -0.5 1,287,089 + Psest_1184 0.86 +0.5 1,287,090 - Psest_1184 0.86 -0.9 1,287,090 - Psest_1184 0.86 +0.6 1,287,090 - Psest_1184 0.86 -1.4
Or see this region's nucleotide sequence