Experiment: Anaerobic growth in rich media with nitrate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2499 and Dsui_2500 are separated by 52 nucleotides Dsui_2500 and Dsui_2501 are separated by 112 nucleotides Dsui_2501 and Dsui_2502 overlap by 4 nucleotides Dsui_2502 and Dsui_2503 are separated by 2 nucleotides
Dsui_2499: Dsui_2499 - MerE protein, at 2,647,150 to 2,647,386
_2499
Dsui_2500: Dsui_2500 - site-specific recombinase, DNA invertase Pin, at 2,647,439 to 2,648,053
_2500
Dsui_2501: Dsui_2501 - TniQ protein, at 2,648,166 to 2,649,332
_2501
Dsui_2502: Dsui_2502 - TniB protein, at 2,649,329 to 2,650,237
_2502
Dsui_2503: Dsui_2503 - Mu transposase/integrase, at 2,650,240 to 2,651,922
_2503
Position (kb)
2648
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2647.222 kb on + strand, within Dsui_2499 at 2647.227 kb on + strand, within Dsui_2499 at 2647.235 kb on - strand, within Dsui_2499 at 2647.299 kb on + strand, within Dsui_2499 at 2647.307 kb on - strand at 2647.324 kb on - strand at 2647.474 kb on - strand at 2647.692 kb on + strand, within Dsui_2500 at 2647.748 kb on + strand, within Dsui_2500 at 2647.769 kb on + strand, within Dsui_2500 at 2647.777 kb on - strand, within Dsui_2500 at 2647.798 kb on + strand at 2647.800 kb on + strand at 2647.823 kb on - strand, within Dsui_2500 at 2648.008 kb on + strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.231 kb on + strand at 2648.239 kb on - strand at 2648.352 kb on + strand, within Dsui_2501 at 2648.419 kb on + strand, within Dsui_2501 at 2648.495 kb on + strand, within Dsui_2501 at 2648.643 kb on - strand at 2648.643 kb on - strand, within Dsui_2501 at 2648.646 kb on + strand, within Dsui_2501 at 2649.070 kb on - strand, within Dsui_2501 at 2649.070 kb on - strand at 2649.364 kb on + strand at 2649.566 kb on + strand, within Dsui_2502 at 2649.703 kb on + strand, within Dsui_2502 at 2649.711 kb on - strand, within Dsui_2502 at 2649.824 kb on - strand, within Dsui_2502 at 2650.079 kb on + strand, within Dsui_2502 at 2650.216 kb on - strand at 2650.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with nitrate electron acceptor remove 2,647,222 + Dsui_2499 0.30 -1.0 2,647,227 + Dsui_2499 0.32 -0.6 2,647,235 - Dsui_2499 0.36 -1.4 2,647,299 + Dsui_2499 0.63 -0.2 2,647,307 - -0.2 2,647,324 - +1.2 2,647,474 - -1.1 2,647,692 + Dsui_2500 0.41 -0.7 2,647,748 + Dsui_2500 0.50 +0.2 2,647,769 + Dsui_2500 0.54 +0.4 2,647,777 - Dsui_2500 0.55 -0.9 2,647,798 + +1.2 2,647,800 + +0.6 2,647,823 - Dsui_2500 0.62 -0.8 2,648,008 + -0.1 2,648,016 - +0.2 2,648,016 - -2.0 2,648,016 - -1.0 2,648,016 - -0.5 2,648,016 - -1.3 2,648,231 + -0.6 2,648,239 - +0.2 2,648,352 + Dsui_2501 0.16 -0.1 2,648,419 + Dsui_2501 0.22 -1.3 2,648,495 + Dsui_2501 0.28 -0.8 2,648,643 - -0.0 2,648,643 - Dsui_2501 0.41 +1.0 2,648,646 + Dsui_2501 0.41 -1.3 2,649,070 - Dsui_2501 0.77 -1.2 2,649,070 - +2.2 2,649,364 + -1.0 2,649,566 + Dsui_2502 0.26 +0.3 2,649,703 + Dsui_2502 0.41 +0.2 2,649,711 - Dsui_2502 0.42 +1.0 2,649,824 - Dsui_2502 0.54 -0.8 2,650,079 + Dsui_2502 0.83 -1.0 2,650,216 - -2.0 2,650,238 + -0.2
Or see this region's nucleotide sequence