Experiment: propionate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaA and SO2249 overlap by 8 nucleotides SO2249 and nagZ are separated by 41 nucleotides nagZ and SO2251 are separated by 81 nucleotides
SO2248: sdaA - L-serine dehydratase 1 (NCBI ptt file), at 2,362,473 to 2,363,849
sdaA
SO2249: SO2249 - hypothetical protein (NCBI ptt file), at 2,363,842 to 2,364,192
SO2249
SO2250: nagZ - beta-hexosaminidase (NCBI ptt file), at 2,364,234 to 2,365,262
nagZ
SO2251: SO2251 - conserved hypothetical protein (NCBI ptt file), at 2,365,344 to 2,365,883
SO2251
Position (kb)
2364
2365
2366 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2363.243 kb on + strand, within sdaA at 2363.373 kb on - strand, within sdaA at 2363.454 kb on - strand, within sdaA at 2363.465 kb on + strand, within sdaA at 2363.681 kb on - strand, within sdaA at 2363.700 kb on + strand, within sdaA at 2363.713 kb on - strand at 2364.270 kb on - strand at 2364.275 kb on - strand at 2364.309 kb on - strand at 2364.972 kb on - strand, within nagZ at 2365.297 kb on + strand at 2365.297 kb on + strand at 2365.527 kb on + strand, within SO2251 at 2365.744 kb on + strand, within SO2251 at 2365.754 kb on + strand, within SO2251 at 2365.875 kb on - strand at 2365.875 kb on - strand at 2365.885 kb on - strand at 2365.909 kb on - strand at 2366.024 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction propionate (C) remove 2,363,243 + sdaA SO2248 0.56 +1.2 2,363,373 - sdaA SO2248 0.65 +1.4 2,363,454 - sdaA SO2248 0.71 +1.5 2,363,465 + sdaA SO2248 0.72 +1.7 2,363,681 - sdaA SO2248 0.88 +2.3 2,363,700 + sdaA SO2248 0.89 +2.2 2,363,713 - +1.1 2,364,270 - -2.1 2,364,275 - -4.2 2,364,309 - -3.4 2,364,972 - nagZ SO2250 0.72 -1.6 2,365,297 + +0.5 2,365,297 + -0.0 2,365,527 + SO2251 0.34 +0.3 2,365,744 + SO2251 0.74 -0.2 2,365,754 + SO2251 0.76 -0.1 2,365,875 - -1.3 2,365,875 - -0.6 2,365,885 - -1.1 2,365,909 - +0.4 2,366,024 + +0.5
Or see this region's nucleotide sequence