Experiment: m.b. methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08960 and PGA1_c08970 are separated by 19 nucleotides PGA1_c08970 and PGA1_c08980 are separated by 205 nucleotides PGA1_c08980 and PGA1_c08990 are separated by 142 nucleotides PGA1_c08990 and PGA1_c09000 overlap by 2 nucleotides PGA1_c09000 and PGA1_c09010 are separated by 220 nucleotides
PGA1_c08960: PGA1_c08960 - DNA repair endonuclease mmcB (DUF1052) (from data) , at 925,190 to 925,645
_c08960
PGA1_c08970: PGA1_c08970 - hypothetical protein, at 925,665 to 925,853
_c08970
PGA1_c08980: PGA1_c08980 - tRNA-His, at 926,059 to 926,135
_c08980
PGA1_c08990: PGA1_c08990 - hypothetical protein, at 926,278 to 926,592
_c08990
PGA1_c09000: PGA1_c09000 - ribosomal large subunit pseudouridine synthase D, at 926,591 to 927,625
_c09000
PGA1_c09010: PGA1_c09010 - RNA polymerase sigma-32 factor RpoH, at 927,846 to 928,742
_c09010
Position (kb)
926
927
928 Strain fitness (log2 ratio)
-2
-1
0
1 at 925.625 kb on + strand at 925.632 kb on - strand at 925.633 kb on - strand at 925.699 kb on + strand, within PGA1_c08970 at 925.840 kb on + strand at 925.873 kb on - strand at 925.880 kb on - strand at 925.901 kb on + strand at 925.929 kb on + strand at 925.944 kb on + strand at 925.952 kb on - strand at 925.952 kb on - strand at 925.963 kb on - strand at 925.963 kb on - strand at 926.233 kb on + strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.293 kb on - strand at 926.293 kb on - strand at 926.340 kb on - strand, within PGA1_c08990 at 926.439 kb on + strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.596 kb on - strand at 926.692 kb on + strand at 926.971 kb on + strand, within PGA1_c09000 at 926.989 kb on + strand, within PGA1_c09000 at 927.011 kb on - strand, within PGA1_c09000 at 927.030 kb on + strand at 927.038 kb on - strand, within PGA1_c09000 at 927.068 kb on + strand, within PGA1_c09000 at 927.129 kb on + strand, within PGA1_c09000 at 927.137 kb on - strand, within PGA1_c09000 at 927.270 kb on - strand, within PGA1_c09000 at 927.270 kb on - strand, within PGA1_c09000 at 927.337 kb on + strand, within PGA1_c09000 at 927.366 kb on + strand, within PGA1_c09000 at 927.405 kb on + strand, within PGA1_c09000 at 927.406 kb on - strand, within PGA1_c09000 at 927.528 kb on + strand at 927.547 kb on - strand at 927.729 kb on - strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.781 kb on - strand at 927.781 kb on - strand at 927.783 kb on + strand at 927.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. methylglyoxal 0.008 vol% remove 925,625 + -0.4 925,632 - -0.6 925,633 - -0.5 925,699 + PGA1_c08970 0.18 -0.6 925,840 + -0.4 925,873 - -0.6 925,880 - -1.3 925,901 + -2.4 925,929 + +0.3 925,944 + -0.9 925,952 - -1.5 925,952 - +1.7 925,963 - -0.9 925,963 - +0.6 926,233 + +1.3 926,243 - +0.5 926,243 - +0.3 926,243 - -0.8 926,243 - -1.0 926,243 - -0.4 926,243 - -1.2 926,243 - +1.1 926,293 - -0.2 926,293 - -0.2 926,340 - PGA1_c08990 0.20 -0.9 926,439 + PGA1_c08990 0.51 -1.7 926,484 - PGA1_c08990 0.65 -1.3 926,484 - PGA1_c08990 0.65 -1.3 926,596 - -0.9 926,692 + -0.7 926,971 + PGA1_c09000 0.37 -1.4 926,989 + PGA1_c09000 0.38 -1.7 927,011 - PGA1_c09000 0.41 +0.2 927,030 + +0.1 927,038 - PGA1_c09000 0.43 +0.3 927,068 + PGA1_c09000 0.46 -0.2 927,129 + PGA1_c09000 0.52 -0.8 927,137 - PGA1_c09000 0.53 -1.9 927,270 - PGA1_c09000 0.66 -2.4 927,270 - PGA1_c09000 0.66 -1.5 927,337 + PGA1_c09000 0.72 -1.2 927,366 + PGA1_c09000 0.75 -1.5 927,405 + PGA1_c09000 0.79 -0.2 927,406 - PGA1_c09000 0.79 +0.1 927,528 + +0.2 927,547 - -0.2 927,729 - -0.9 927,773 + -1.9 927,773 + -0.1 927,773 + +0.3 927,773 + +1.1 927,773 + -1.0 927,781 - +0.5 927,781 - +0.2 927,783 + -1.0 927,785 + -0.7
Or see this region's nucleotide sequence