Experiment: m.b. methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04930 and PGA1_c04940 are separated by 112 nucleotides PGA1_c04940 and PGA1_c04950 are separated by 296 nucleotides PGA1_c04950 and PGA1_c04960 are separated by 154 nucleotides
PGA1_c04930: PGA1_c04930 - tryptophan synthase alpha chain, at 484,569 to 485,360
_c04930
PGA1_c04940: PGA1_c04940 - Lipid A 3-O-deacylase (PagL)., at 485,473 to 485,985
_c04940
PGA1_c04950: PGA1_c04950 - GTP-dependent nucleic acid-binding protein EngD, at 486,282 to 487,379
_c04950
PGA1_c04960: PGA1_c04960 - putative non-specific ribonucleoside hydrolase, at 487,534 to 488,454
_c04960
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 485.363 kb on - strand at 485.392 kb on - strand at 485.412 kb on + strand at 485.420 kb on - strand at 485.420 kb on - strand at 485.426 kb on + strand at 485.453 kb on + strand at 485.517 kb on + strand at 485.522 kb on + strand at 485.530 kb on - strand, within PGA1_c04940 at 485.548 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.593 kb on + strand, within PGA1_c04940 at 485.604 kb on + strand, within PGA1_c04940 at 485.649 kb on + strand, within PGA1_c04940 at 485.676 kb on + strand, within PGA1_c04940 at 485.684 kb on - strand, within PGA1_c04940 at 485.740 kb on + strand, within PGA1_c04940 at 485.746 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.904 kb on - strand, within PGA1_c04940 at 485.943 kb on - strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.964 kb on + strand at 485.992 kb on + strand at 485.992 kb on + strand at 485.999 kb on - strand at 486.000 kb on - strand at 486.073 kb on - strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.140 kb on - strand at 486.173 kb on + strand at 486.190 kb on + strand at 486.202 kb on + strand at 486.212 kb on - strand at 486.216 kb on - strand at 486.234 kb on + strand at 486.244 kb on + strand at 486.255 kb on + strand at 486.273 kb on - strand at 486.520 kb on - strand, within PGA1_c04950 at 486.720 kb on + strand, within PGA1_c04950 at 486.969 kb on + strand, within PGA1_c04950 at 487.070 kb on + strand, within PGA1_c04950 at 487.381 kb on - strand at 487.383 kb on + strand at 487.454 kb on - strand at 487.472 kb on + strand at 487.480 kb on - strand at 487.509 kb on + strand at 487.509 kb on + strand at 487.517 kb on - strand at 487.566 kb on - strand at 487.573 kb on - strand at 487.610 kb on + strand at 487.653 kb on + strand, within PGA1_c04960 at 487.699 kb on + strand, within PGA1_c04960 at 487.700 kb on + strand, within PGA1_c04960 at 487.707 kb on - strand, within PGA1_c04960 at 487.708 kb on - strand, within PGA1_c04960 at 487.735 kb on + strand, within PGA1_c04960 at 487.759 kb on + strand, within PGA1_c04960 at 487.778 kb on + strand, within PGA1_c04960 at 487.820 kb on - strand, within PGA1_c04960 at 487.828 kb on - strand, within PGA1_c04960 at 487.832 kb on - strand, within PGA1_c04960 at 487.923 kb on - strand, within PGA1_c04960 at 487.935 kb on + strand, within PGA1_c04960 at 487.945 kb on + strand, within PGA1_c04960 at 487.959 kb on + strand, within PGA1_c04960 at 487.980 kb on - strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 488.083 kb on - strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.115 kb on - strand, within PGA1_c04960 at 488.126 kb on - strand, within PGA1_c04960 at 488.146 kb on + strand, within PGA1_c04960 at 488.159 kb on + strand, within PGA1_c04960 at 488.164 kb on + strand, within PGA1_c04960 at 488.167 kb on - strand, within PGA1_c04960 at 488.177 kb on - strand, within PGA1_c04960 at 488.284 kb on + strand, within PGA1_c04960 at 488.286 kb on + strand, within PGA1_c04960 at 488.329 kb on + strand, within PGA1_c04960 at 488.332 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. methylglyoxal 0.008 vol% remove 485,363 - +1.8 485,392 - +1.2 485,412 + +1.2 485,420 - -1.0 485,420 - +0.2 485,426 + +0.5 485,453 + -0.3 485,517 + -2.7 485,522 + -0.7 485,530 - PGA1_c04940 0.11 -1.1 485,548 + PGA1_c04940 0.15 -1.6 485,557 + PGA1_c04940 0.16 -3.1 485,557 + PGA1_c04940 0.16 +0.8 485,557 + PGA1_c04940 0.16 -1.4 485,557 + PGA1_c04940 0.16 +1.2 485,557 + PGA1_c04940 0.16 -1.8 485,562 + PGA1_c04940 0.17 -0.7 485,562 + PGA1_c04940 0.17 +1.8 485,565 - PGA1_c04940 0.18 -2.6 485,565 - PGA1_c04940 0.18 -0.8 485,593 + PGA1_c04940 0.23 -1.0 485,604 + PGA1_c04940 0.26 -1.8 485,649 + PGA1_c04940 0.34 -0.6 485,676 + PGA1_c04940 0.40 -1.7 485,684 - PGA1_c04940 0.41 +0.8 485,740 + PGA1_c04940 0.52 -0.6 485,746 - PGA1_c04940 0.53 +0.1 485,766 - PGA1_c04940 0.57 +0.2 485,766 - PGA1_c04940 0.57 -2.1 485,853 + PGA1_c04940 0.74 -1.1 485,853 + PGA1_c04940 0.74 -0.9 485,904 - PGA1_c04940 0.84 +0.2 485,943 - -0.5 485,947 + -0.6 485,947 + +0.4 485,947 + -0.0 485,947 + -1.2 485,947 + -0.4 485,955 - +2.1 485,955 - -1.4 485,955 - +1.2 485,964 + +0.4 485,992 + +0.0 485,992 + -0.2 485,999 - +0.0 486,000 - +1.6 486,073 - +0.2 486,076 + -0.3 486,076 + -1.0 486,076 + +1.2 486,076 + -2.1 486,076 + -2.1 486,076 + -1.7 486,076 + -0.8 486,076 + +0.9 486,076 + -0.8 486,076 + -0.1 486,076 + +0.5 486,076 + -0.7 486,076 + +1.0 486,076 + -1.9 486,076 + +0.2 486,076 + +0.2 486,076 + +0.5 486,076 + -0.1 486,076 + -0.5 486,076 + +0.6 486,076 + -1.4 486,076 + +0.5 486,076 + +1.2 486,076 + -2.3 486,076 + +0.5 486,076 + -2.0 486,076 + -1.1 486,076 + -1.1 486,076 + -0.7 486,084 - -1.5 486,084 - +0.3 486,084 - +0.4 486,084 - -0.4 486,084 - -0.8 486,084 - +0.5 486,084 - +0.2 486,084 - +1.2 486,084 - -1.0 486,084 - +1.5 486,084 - +0.0 486,084 - -0.4 486,084 - -2.7 486,084 - -1.6 486,084 - -0.8 486,084 - -0.2 486,084 - +0.0 486,084 - -0.5 486,084 - -1.8 486,084 - -2.7 486,084 - -1.2 486,084 - +0.8 486,084 - -0.3 486,084 - +2.2 486,084 - +0.6 486,084 - -2.7 486,084 - +1.3 486,084 - +0.2 486,084 - -2.3 486,084 - +1.4 486,084 - +0.2 486,084 - -1.1 486,084 - -0.0 486,084 - -1.4 486,084 - +1.1 486,084 - -0.3 486,084 - +1.2 486,140 - +0.5 486,173 + -0.7 486,190 + -2.3 486,202 + -0.7 486,212 - -0.3 486,216 - +0.4 486,234 + +0.4 486,244 + -0.4 486,255 + -0.3 486,273 - -1.6 486,520 - PGA1_c04950 0.22 -1.6 486,720 + PGA1_c04950 0.40 -0.1 486,969 + PGA1_c04950 0.63 -1.2 487,070 + PGA1_c04950 0.72 -1.4 487,381 - -0.8 487,383 + -0.4 487,454 - +1.2 487,472 + -0.7 487,480 - +0.2 487,509 + +1.1 487,509 + +1.3 487,517 - -0.2 487,566 - +1.7 487,573 - +1.3 487,610 + +1.3 487,653 + PGA1_c04960 0.13 -0.4 487,699 + PGA1_c04960 0.18 +0.2 487,700 + PGA1_c04960 0.18 +2.0 487,707 - PGA1_c04960 0.19 -0.7 487,708 - PGA1_c04960 0.19 -0.2 487,735 + PGA1_c04960 0.22 -0.4 487,759 + PGA1_c04960 0.24 +0.1 487,778 + PGA1_c04960 0.26 +0.9 487,820 - PGA1_c04960 0.31 +1.2 487,828 - PGA1_c04960 0.32 +1.0 487,832 - PGA1_c04960 0.32 +1.2 487,923 - PGA1_c04960 0.42 -0.8 487,935 + PGA1_c04960 0.44 +1.1 487,945 + PGA1_c04960 0.45 -1.5 487,959 + PGA1_c04960 0.46 -0.2 487,980 - PGA1_c04960 0.48 -0.4 487,988 + PGA1_c04960 0.49 -1.2 487,988 + PGA1_c04960 0.49 +0.4 487,988 + PGA1_c04960 0.49 +0.1 487,996 - PGA1_c04960 0.50 +0.8 487,996 - PGA1_c04960 0.50 -0.0 487,996 - PGA1_c04960 0.50 +0.0 487,996 - PGA1_c04960 0.50 +0.1 488,083 - PGA1_c04960 0.60 -0.8 488,096 + PGA1_c04960 0.61 +1.2 488,096 + PGA1_c04960 0.61 +0.0 488,096 + PGA1_c04960 0.61 +1.7 488,096 + PGA1_c04960 0.61 +0.7 488,096 + PGA1_c04960 0.61 +0.7 488,096 + PGA1_c04960 0.61 +0.4 488,104 - PGA1_c04960 0.62 -0.8 488,104 - PGA1_c04960 0.62 +0.9 488,115 - PGA1_c04960 0.63 +0.9 488,126 - PGA1_c04960 0.64 +0.9 488,146 + PGA1_c04960 0.66 -0.5 488,159 + PGA1_c04960 0.68 -0.2 488,164 + PGA1_c04960 0.68 -0.4 488,167 - PGA1_c04960 0.69 +2.1 488,177 - PGA1_c04960 0.70 +2.9 488,284 + PGA1_c04960 0.81 -0.2 488,286 + PGA1_c04960 0.82 +0.3 488,329 + PGA1_c04960 0.86 -0.8 488,332 - PGA1_c04960 0.87 +1.0 488,337 - PGA1_c04960 0.87 -0.5 488,337 - PGA1_c04960 0.87 +1.5 488,366 + +0.6
Or see this region's nucleotide sequence