Experiment: m.b. methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01370 and PGA1_262p01380 overlap by 4 nucleotides PGA1_262p01380 and PGA1_262p01390 are separated by 3 nucleotides PGA1_262p01390 and PGA1_262p01400 are separated by 144 nucleotides
PGA1_262p01370: PGA1_262p01370 - putative dioxygenase, at 147,465 to 148,619
_262p01370
PGA1_262p01380: PGA1_262p01380 - aldehyde dehydrogenase, at 148,616 to 150,067
_262p01380
PGA1_262p01390: PGA1_262p01390 - hypothetical protein, at 150,071 to 150,310
_262p01390
PGA1_262p01400: PGA1_262p01400 - dimethylglycine dehydrogenase DmgdH, at 150,455 to 152,875
_262p01400
Position (kb)
148
149
150
151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 147.628 kb on - strand, within PGA1_262p01370 at 147.690 kb on + strand, within PGA1_262p01370 at 147.712 kb on + strand, within PGA1_262p01370 at 147.778 kb on + strand, within PGA1_262p01370 at 147.786 kb on - strand, within PGA1_262p01370 at 147.846 kb on + strand, within PGA1_262p01370 at 147.859 kb on - strand, within PGA1_262p01370 at 147.893 kb on - strand, within PGA1_262p01370 at 148.068 kb on - strand, within PGA1_262p01370 at 148.111 kb on - strand, within PGA1_262p01370 at 148.119 kb on + strand, within PGA1_262p01370 at 148.121 kb on - strand, within PGA1_262p01370 at 148.177 kb on + strand, within PGA1_262p01370 at 148.192 kb on + strand, within PGA1_262p01370 at 148.304 kb on + strand, within PGA1_262p01370 at 148.379 kb on - strand, within PGA1_262p01370 at 148.441 kb on - strand, within PGA1_262p01370 at 148.578 kb on - strand at 148.643 kb on + strand at 148.765 kb on - strand, within PGA1_262p01380 at 148.775 kb on - strand, within PGA1_262p01380 at 148.791 kb on + strand, within PGA1_262p01380 at 149.019 kb on + strand, within PGA1_262p01380 at 149.019 kb on + strand, within PGA1_262p01380 at 149.103 kb on - strand, within PGA1_262p01380 at 149.117 kb on + strand, within PGA1_262p01380 at 149.124 kb on - strand, within PGA1_262p01380 at 149.125 kb on - strand, within PGA1_262p01380 at 149.471 kb on + strand, within PGA1_262p01380 at 149.536 kb on - strand, within PGA1_262p01380 at 149.536 kb on - strand, within PGA1_262p01380 at 149.726 kb on + strand, within PGA1_262p01380 at 149.726 kb on + strand, within PGA1_262p01380 at 149.918 kb on + strand, within PGA1_262p01380 at 149.926 kb on - strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.019 kb on + strand at 150.027 kb on - strand at 150.027 kb on - strand at 150.066 kb on + strand at 150.092 kb on - strand at 150.134 kb on - strand, within PGA1_262p01390 at 150.150 kb on - strand, within PGA1_262p01390 at 150.267 kb on - strand, within PGA1_262p01390 at 150.278 kb on + strand, within PGA1_262p01390 at 150.286 kb on - strand, within PGA1_262p01390 at 150.286 kb on - strand, within PGA1_262p01390 at 150.358 kb on + strand at 150.366 kb on - strand at 150.447 kb on + strand at 150.470 kb on - strand at 150.558 kb on + strand at 150.588 kb on + strand at 150.689 kb on + strand at 150.697 kb on - strand, within PGA1_262p01400 at 150.706 kb on + strand, within PGA1_262p01400 at 150.729 kb on - strand, within PGA1_262p01400 at 150.734 kb on - strand, within PGA1_262p01400 at 150.778 kb on + strand, within PGA1_262p01400 at 150.813 kb on + strand, within PGA1_262p01400 at 150.823 kb on + strand, within PGA1_262p01400 at 150.830 kb on + strand, within PGA1_262p01400 at 151.036 kb on - strand, within PGA1_262p01400
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. methylglyoxal 0.008 vol% remove 147,628 - PGA1_262p01370 0.14 -1.5 147,690 + PGA1_262p01370 0.19 +1.2 147,712 + PGA1_262p01370 0.21 +1.0 147,778 + PGA1_262p01370 0.27 +0.5 147,786 - PGA1_262p01370 0.28 +0.5 147,846 + PGA1_262p01370 0.33 +0.6 147,859 - PGA1_262p01370 0.34 +1.2 147,893 - PGA1_262p01370 0.37 -0.0 148,068 - PGA1_262p01370 0.52 -0.2 148,111 - PGA1_262p01370 0.56 +0.3 148,119 + PGA1_262p01370 0.57 -1.1 148,121 - PGA1_262p01370 0.57 -0.3 148,177 + PGA1_262p01370 0.62 +0.9 148,192 + PGA1_262p01370 0.63 -0.0 148,304 + PGA1_262p01370 0.73 -1.8 148,379 - PGA1_262p01370 0.79 -0.8 148,441 - PGA1_262p01370 0.85 -1.2 148,578 - +0.5 148,643 + -1.4 148,765 - PGA1_262p01380 0.10 +0.4 148,775 - PGA1_262p01380 0.11 -2.5 148,791 + PGA1_262p01380 0.12 -0.6 149,019 + PGA1_262p01380 0.28 +0.0 149,019 + PGA1_262p01380 0.28 +0.9 149,103 - PGA1_262p01380 0.34 +0.4 149,117 + PGA1_262p01380 0.35 +0.1 149,124 - PGA1_262p01380 0.35 +0.4 149,125 - PGA1_262p01380 0.35 -2.1 149,471 + PGA1_262p01380 0.59 +0.1 149,536 - PGA1_262p01380 0.63 -0.5 149,536 - PGA1_262p01380 0.63 +0.9 149,726 + PGA1_262p01380 0.76 -0.9 149,726 + PGA1_262p01380 0.76 +0.0 149,918 + PGA1_262p01380 0.90 +0.0 149,926 - -1.6 150,019 + +0.0 150,019 + +0.2 150,019 + +0.9 150,019 + -0.0 150,027 - +1.7 150,027 - -1.9 150,066 + -0.8 150,092 - -2.9 150,134 - PGA1_262p01390 0.26 +1.3 150,150 - PGA1_262p01390 0.33 +0.5 150,267 - PGA1_262p01390 0.82 -1.0 150,278 + PGA1_262p01390 0.86 +0.0 150,286 - PGA1_262p01390 0.90 -0.8 150,286 - PGA1_262p01390 0.90 +1.2 150,358 + -2.5 150,366 - -0.1 150,447 + -0.6 150,470 - -0.7 150,558 + +1.2 150,588 + +0.2 150,689 + +0.9 150,697 - PGA1_262p01400 0.10 +0.5 150,706 + PGA1_262p01400 0.10 -0.7 150,729 - PGA1_262p01400 0.11 +0.5 150,734 - PGA1_262p01400 0.12 +2.2 150,778 + PGA1_262p01400 0.13 -1.7 150,813 + PGA1_262p01400 0.15 -0.1 150,823 + PGA1_262p01400 0.15 -0.2 150,830 + PGA1_262p01400 0.15 +0.4 151,036 - PGA1_262p01400 0.24 +0.4
Or see this region's nucleotide sequence