Experiment: Anaerobic growth in rich media with nitrate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3312 and Dsui_3313 are separated by 63 nucleotides Dsui_3313 and Dsui_3314 are separated by 108 nucleotides Dsui_3314 and Dsui_3315 are separated by 10 nucleotides Dsui_3315 and Dsui_3316 are separated by 18 nucleotides Dsui_3316 and Dsui_3317 are separated by 105 nucleotides
Dsui_3312: Dsui_3312 - hypothetical protein, at 3,554,286 to 3,554,783
_3312
Dsui_3313: Dsui_3313 - hypothetical protein, at 3,554,847 to 3,555,242
_3313
Dsui_3314: Dsui_3314 - biopolymer transport protein, at 3,555,351 to 3,555,767
_3314
Dsui_3315: Dsui_3315 - biopolymer transport protein, at 3,555,778 to 3,556,497
_3315
Dsui_3316: Dsui_3316 - Gram-negative bacterial tonB protein, at 3,556,516 to 3,557,289
_3316
Dsui_3317: Dsui_3317 - cytochrome c551/c552, at 3,557,395 to 3,557,733
_3317
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3554.845 kb on + strand at 3554.853 kb on - strand at 3554.855 kb on + strand at 3554.855 kb on + strand at 3554.863 kb on - strand at 3554.869 kb on + strand at 3554.911 kb on - strand, within Dsui_3313 at 3555.015 kb on - strand, within Dsui_3313 at 3555.080 kb on + strand at 3555.105 kb on + strand, within Dsui_3313 at 3555.113 kb on - strand, within Dsui_3313 at 3555.225 kb on - strand at 3555.251 kb on + strand at 3555.260 kb on - strand at 3555.277 kb on + strand at 3555.337 kb on + strand at 3555.342 kb on + strand at 3555.561 kb on + strand, within Dsui_3314 at 3555.589 kb on - strand, within Dsui_3314 at 3555.696 kb on + strand, within Dsui_3314 at 3555.808 kb on - strand at 3555.820 kb on - strand at 3555.830 kb on + strand at 3555.908 kb on - strand, within Dsui_3315 at 3555.961 kb on + strand at 3556.002 kb on - strand, within Dsui_3315 at 3556.111 kb on + strand at 3556.119 kb on - strand, within Dsui_3315 at 3556.124 kb on - strand, within Dsui_3315 at 3556.223 kb on - strand, within Dsui_3315 at 3556.230 kb on - strand, within Dsui_3315 at 3556.323 kb on + strand, within Dsui_3315 at 3556.381 kb on - strand, within Dsui_3315 at 3556.449 kb on + strand at 3556.511 kb on - strand at 3556.513 kb on + strand at 3556.587 kb on - strand at 3556.632 kb on + strand at 3556.673 kb on + strand at 3556.762 kb on - strand, within Dsui_3316 at 3556.798 kb on - strand, within Dsui_3316 at 3556.802 kb on - strand, within Dsui_3316 at 3556.845 kb on - strand, within Dsui_3316 at 3556.964 kb on + strand, within Dsui_3316 at 3556.972 kb on - strand, within Dsui_3316 at 3557.093 kb on + strand at 3557.116 kb on - strand, within Dsui_3316 at 3557.177 kb on - strand, within Dsui_3316 at 3557.187 kb on - strand, within Dsui_3316 at 3557.239 kb on + strand at 3557.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with nitrate electron acceptor remove 3,554,845 + -0.3 3,554,853 - +0.0 3,554,855 + +0.4 3,554,855 + -0.3 3,554,863 - -0.7 3,554,869 + +1.2 3,554,911 - Dsui_3313 0.16 -0.1 3,555,015 - Dsui_3313 0.42 +0.1 3,555,080 + +2.2 3,555,105 + Dsui_3313 0.65 +0.8 3,555,113 - Dsui_3313 0.67 -0.4 3,555,225 - -0.1 3,555,251 + -1.7 3,555,260 - +0.6 3,555,277 + -0.1 3,555,337 + -0.5 3,555,342 + +2.2 3,555,561 + Dsui_3314 0.50 -0.2 3,555,589 - Dsui_3314 0.57 -0.5 3,555,696 + Dsui_3314 0.83 +0.2 3,555,808 - -0.3 3,555,820 - -0.2 3,555,830 + +0.2 3,555,908 - Dsui_3315 0.18 -0.6 3,555,961 + +0.7 3,556,002 - Dsui_3315 0.31 +0.0 3,556,111 + +0.9 3,556,119 - Dsui_3315 0.47 +0.2 3,556,124 - Dsui_3315 0.48 +0.1 3,556,223 - Dsui_3315 0.62 -0.8 3,556,230 - Dsui_3315 0.63 +0.8 3,556,323 + Dsui_3315 0.76 -1.3 3,556,381 - Dsui_3315 0.84 +1.0 3,556,449 + +0.2 3,556,511 - -1.2 3,556,513 + -0.1 3,556,587 - -2.0 3,556,632 + +0.7 3,556,673 + +0.7 3,556,762 - Dsui_3316 0.32 -0.5 3,556,798 - Dsui_3316 0.36 +0.4 3,556,802 - Dsui_3316 0.37 +0.1 3,556,845 - Dsui_3316 0.43 -0.3 3,556,964 + Dsui_3316 0.58 +0.2 3,556,972 - Dsui_3316 0.59 +0.0 3,557,093 + +0.2 3,557,116 - Dsui_3316 0.78 +0.5 3,557,177 - Dsui_3316 0.85 -0.5 3,557,187 - Dsui_3316 0.87 +0.3 3,557,239 + -1.1 3,557,294 + -1.1
Or see this region's nucleotide sequence