Experiment: Anaerobic growth in rich media with nitrate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0982 and Dsui_0983 are separated by 188 nucleotides Dsui_0983 and Dsui_0984 are separated by 18 nucleotides Dsui_0984 and Dsui_0985 are separated by 62 nucleotides
Dsui_0982: Dsui_0982 - acetyl/propionyl-CoA carboxylase, alpha subunit, at 1,008,783 to 1,010,786
_0982
Dsui_0983: Dsui_0983 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,010,975 to 1,013,275
_0983
Dsui_0984: Dsui_0984 - isopropylmalate/homocitrate/citramalate synthase, at 1,013,294 to 1,014,202
_0984
Dsui_0985: Dsui_0985 - putative Fe-S protein, at 1,014,265 to 1,014,480
_0985
Position (kb)
1010
1011
1012
1013
1014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1009.978 kb on - strand, within Dsui_0982 at 1010.003 kb on - strand, within Dsui_0982 at 1010.083 kb on + strand, within Dsui_0982 at 1010.390 kb on + strand, within Dsui_0982 at 1010.537 kb on - strand, within Dsui_0982 at 1010.615 kb on + strand at 1010.686 kb on + strand at 1010.754 kb on - strand at 1010.780 kb on + strand at 1010.863 kb on + strand at 1010.876 kb on - strand at 1011.031 kb on + strand at 1011.114 kb on + strand at 1011.132 kb on + strand at 1011.132 kb on + strand at 1011.369 kb on - strand, within Dsui_0983 at 1011.385 kb on + strand, within Dsui_0983 at 1011.488 kb on - strand, within Dsui_0983 at 1011.507 kb on + strand, within Dsui_0983 at 1011.517 kb on + strand, within Dsui_0983 at 1011.635 kb on - strand, within Dsui_0983 at 1011.642 kb on + strand, within Dsui_0983 at 1011.706 kb on + strand, within Dsui_0983 at 1011.767 kb on - strand, within Dsui_0983 at 1011.957 kb on + strand at 1011.965 kb on - strand, within Dsui_0983 at 1011.982 kb on - strand, within Dsui_0983 at 1012.022 kb on + strand, within Dsui_0983 at 1012.036 kb on + strand, within Dsui_0983 at 1012.048 kb on + strand, within Dsui_0983 at 1012.048 kb on + strand, within Dsui_0983 at 1012.567 kb on - strand, within Dsui_0983 at 1012.789 kb on - strand at 1012.810 kb on - strand, within Dsui_0983 at 1012.930 kb on - strand, within Dsui_0983 at 1012.963 kb on + strand, within Dsui_0983 at 1012.971 kb on - strand, within Dsui_0983 at 1013.081 kb on + strand at 1013.089 kb on - strand at 1013.126 kb on - strand at 1013.144 kb on + strand at 1013.149 kb on + strand at 1013.149 kb on + strand at 1013.249 kb on + strand at 1013.311 kb on + strand at 1013.331 kb on + strand at 1013.382 kb on + strand at 1013.531 kb on - strand, within Dsui_0984 at 1013.531 kb on - strand, within Dsui_0984 at 1013.606 kb on + strand, within Dsui_0984 at 1013.640 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.713 kb on - strand, within Dsui_0984 at 1013.825 kb on - strand, within Dsui_0984 at 1013.829 kb on - strand, within Dsui_0984 at 1013.837 kb on - strand at 1013.925 kb on + strand, within Dsui_0984 at 1013.944 kb on - strand, within Dsui_0984 at 1013.966 kb on - strand at 1014.067 kb on + strand, within Dsui_0984 at 1014.108 kb on + strand, within Dsui_0984 at 1014.262 kb on + strand at 1014.262 kb on + strand at 1014.274 kb on + strand at 1014.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with nitrate electron acceptor remove 1,009,978 - Dsui_0982 0.60 +0.9 1,010,003 - Dsui_0982 0.61 -0.3 1,010,083 + Dsui_0982 0.65 -2.2 1,010,390 + Dsui_0982 0.80 -0.5 1,010,537 - Dsui_0982 0.88 -0.2 1,010,615 + +1.3 1,010,686 + -0.6 1,010,754 - +0.9 1,010,780 + -1.1 1,010,863 + -1.3 1,010,876 - +2.3 1,011,031 + -0.1 1,011,114 + -1.3 1,011,132 + -1.5 1,011,132 + +0.9 1,011,369 - Dsui_0983 0.17 +0.7 1,011,385 + Dsui_0983 0.18 +0.9 1,011,488 - Dsui_0983 0.22 +0.7 1,011,507 + Dsui_0983 0.23 -0.1 1,011,517 + Dsui_0983 0.24 -1.5 1,011,635 - Dsui_0983 0.29 -1.6 1,011,642 + Dsui_0983 0.29 -0.4 1,011,706 + Dsui_0983 0.32 +0.3 1,011,767 - Dsui_0983 0.34 +0.4 1,011,957 + +0.3 1,011,965 - Dsui_0983 0.43 -1.5 1,011,982 - Dsui_0983 0.44 -0.3 1,012,022 + Dsui_0983 0.46 +1.7 1,012,036 + Dsui_0983 0.46 -1.6 1,012,048 + Dsui_0983 0.47 +2.3 1,012,048 + Dsui_0983 0.47 -0.6 1,012,567 - Dsui_0983 0.69 +0.2 1,012,789 - +1.3 1,012,810 - Dsui_0983 0.80 -0.2 1,012,930 - Dsui_0983 0.85 -0.9 1,012,963 + Dsui_0983 0.86 -0.3 1,012,971 - Dsui_0983 0.87 +0.7 1,013,081 + -0.2 1,013,089 - -0.5 1,013,126 - -0.5 1,013,144 + -0.5 1,013,149 + -1.4 1,013,149 + -1.6 1,013,249 + -1.5 1,013,311 + -0.3 1,013,331 + -1.4 1,013,382 + -0.3 1,013,531 - Dsui_0984 0.26 +0.9 1,013,531 - Dsui_0984 0.26 -1.4 1,013,606 + Dsui_0984 0.34 +0.4 1,013,640 - Dsui_0984 0.38 +1.6 1,013,713 - Dsui_0984 0.46 -0.6 1,013,713 - Dsui_0984 0.46 -0.9 1,013,825 - Dsui_0984 0.58 -0.7 1,013,829 - Dsui_0984 0.59 -1.2 1,013,837 - +1.3 1,013,925 + Dsui_0984 0.69 -0.2 1,013,944 - Dsui_0984 0.72 +0.1 1,013,966 - -0.5 1,014,067 + Dsui_0984 0.85 -1.0 1,014,108 + Dsui_0984 0.90 -0.5 1,014,262 + +0.3 1,014,262 + -1.1 1,014,274 + -0.3 1,014,274 + +0.5
Or see this region's nucleotide sequence