Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrD-2 and nqrE-2 are separated by 5 nucleotides nqrE-2 and nqrF-2 are separated by 46 nucleotides nqrF-2 and apbE are separated by 99 nucleotides
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
SO1108: nqrF-2 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 1,150,843 to 1,152,099
nqrF-2
SO1109: apbE - thiamin biosynthesis lipoprotein ApbE (NCBI ptt file), at 1,152,199 to 1,153,242
apbE
Position (kb) 1150
1151
1152
1153 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2 at 1150.556 kb on - strand, within nqrE-2 at 1150.638 kb on + strand, within nqrE-2 at 1152.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 1,150,157 - -3.3 1,150,323 + nqrE-2 SO1107 0.22 -3.7 1,150,427 - nqrE-2 SO1107 0.39 -2.5 1,150,539 + nqrE-2 SO1107 0.58 -3.1 1,150,556 - nqrE-2 SO1107 0.60 -2.4 1,150,638 + nqrE-2 SO1107 0.74 -3.9 1,152,137 + +0.2
Or see this region's nucleotide sequence