Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 17 nucleotides ruvA and ruvC overlap by 4 nucleotides ruvC and Sama_1842 are separated by 3 nucleotides
Sama_1839: ruvB - Holliday junction DNA helicase RuvB (RefSeq), at 2,240,347 to 2,241,351
ruvB
Sama_1840: ruvA - Holliday junction DNA helicase RuvA (RefSeq), at 2,241,369 to 2,241,986
ruvA
Sama_1841: ruvC - Holliday junction resolvase (RefSeq), at 2,241,983 to 2,242,504
ruvC
Sama_1842: Sama_1842 - hypothetical protein (RefSeq), at 2,242,508 to 2,243,254
_1842
Position (kb)
2241
2242 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2240.565 kb on - strand, within ruvB at 2240.886 kb on + strand, within ruvB at 2240.911 kb on - strand, within ruvB at 2240.911 kb on - strand, within ruvB at 2240.998 kb on + strand, within ruvB at 2241.150 kb on + strand, within ruvB at 2241.150 kb on + strand, within ruvB at 2241.151 kb on - strand, within ruvB at 2241.175 kb on - strand, within ruvB at 2241.180 kb on + strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.272 kb on - strand at 2241.272 kb on - strand at 2241.355 kb on - strand at 2241.371 kb on - strand at 2241.371 kb on - strand at 2241.399 kb on - strand at 2241.692 kb on + strand, within ruvA at 2241.931 kb on - strand at 2241.982 kb on - strand at 2241.991 kb on - strand at 2241.991 kb on - strand at 2242.406 kb on - strand, within ruvC at 2242.422 kb on + strand, within ruvC at 2242.422 kb on + strand, within ruvC at 2242.549 kb on + strand at 2242.554 kb on + strand at 2242.555 kb on - strand at 2242.555 kb on - strand at 2242.555 kb on - strand at 2242.730 kb on - strand, within Sama_1842 at 2242.730 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.836 kb on - strand, within Sama_1842 at 2242.836 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 2,240,565 - ruvB Sama_1839 0.22 -2.6 2,240,886 + ruvB Sama_1839 0.54 -2.2 2,240,911 - ruvB Sama_1839 0.56 -0.8 2,240,911 - ruvB Sama_1839 0.56 -2.0 2,240,998 + ruvB Sama_1839 0.65 -3.0 2,241,150 + ruvB Sama_1839 0.80 -0.8 2,241,150 + ruvB Sama_1839 0.80 -3.3 2,241,151 - ruvB Sama_1839 0.80 -2.4 2,241,175 - ruvB Sama_1839 0.82 -3.3 2,241,180 + ruvB Sama_1839 0.83 -2.4 2,241,181 - ruvB Sama_1839 0.83 -3.0 2,241,181 - ruvB Sama_1839 0.83 -2.4 2,241,181 - ruvB Sama_1839 0.83 -0.6 2,241,272 - -0.8 2,241,272 - +0.2 2,241,355 - -0.4 2,241,371 - +0.5 2,241,371 - -0.4 2,241,399 - -2.4 2,241,692 + ruvA Sama_1840 0.52 +0.2 2,241,931 - -2.2 2,241,982 - -1.8 2,241,991 - +0.2 2,241,991 - -3.0 2,242,406 - ruvC Sama_1841 0.81 +0.2 2,242,422 + ruvC Sama_1841 0.84 -0.6 2,242,422 + ruvC Sama_1841 0.84 -0.8 2,242,549 + -1.2 2,242,554 + -0.8 2,242,555 - -0.1 2,242,555 - -0.6 2,242,555 - -0.8 2,242,730 - Sama_1842 0.30 -0.2 2,242,730 - Sama_1842 0.30 +1.8 2,242,735 - Sama_1842 0.30 +0.6 2,242,735 - Sama_1842 0.30 -0.2 2,242,735 - Sama_1842 0.30 -1.6 2,242,836 - Sama_1842 0.44 -0.8 2,242,836 - Sama_1842 0.44 +0.6 2,242,901 - Sama_1842 0.53 +1.2 2,242,901 - Sama_1842 0.53 +1.2 2,242,901 - Sama_1842 0.53 -1.8
Or see this region's nucleotide sequence