Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshA and SO3560 are separated by 192 nucleotides SO3560 and SO3561 are separated by 141 nucleotides SO3561 and SO3562 are separated by 287 nucleotides
SO3559: gshA - glutamate--cysteine ligase (NCBI ptt file), at 3,716,992 to 3,718,686
gshA
SO3560: SO3560 - peptidase, M16 family (NCBI ptt file), at 3,718,879 to 3,721,728
SO3560
SO3561: SO3561 - conserved hypothetical protein (NCBI ptt file), at 3,721,870 to 3,722,295
SO3561
SO3562: SO3562 - proton/glutamate symporter, putative (NCBI ptt file), at 3,722,583 to 3,723,824
SO3562
Position (kb)
3718
3719
3720
3721
3722 Strain fitness (log2 ratio)
-2
-1
0
1 at 3717.880 kb on - strand, within gshA at 3717.982 kb on - strand, within gshA at 3718.358 kb on + strand, within gshA at 3718.402 kb on + strand, within gshA at 3718.410 kb on - strand, within gshA at 3718.410 kb on - strand, within gshA at 3718.410 kb on - strand, within gshA at 3718.524 kb on - strand at 3718.580 kb on - strand at 3718.610 kb on - strand at 3718.746 kb on + strand at 3718.746 kb on + strand at 3718.754 kb on + strand at 3718.778 kb on - strand at 3718.844 kb on + strand at 3718.906 kb on + strand at 3718.914 kb on - strand at 3719.130 kb on + strand at 3719.138 kb on - strand at 3719.163 kb on + strand at 3719.315 kb on + strand, within SO3560 at 3719.337 kb on + strand, within SO3560 at 3719.345 kb on - strand, within SO3560 at 3719.424 kb on - strand, within SO3560 at 3719.465 kb on - strand, within SO3560 at 3719.470 kb on - strand, within SO3560 at 3719.544 kb on - strand, within SO3560 at 3719.585 kb on + strand, within SO3560 at 3719.588 kb on - strand, within SO3560 at 3719.632 kb on + strand, within SO3560 at 3719.685 kb on + strand, within SO3560 at 3719.693 kb on - strand, within SO3560 at 3719.761 kb on - strand, within SO3560 at 3719.854 kb on - strand, within SO3560 at 3719.908 kb on + strand, within SO3560 at 3719.956 kb on + strand, within SO3560 at 3719.964 kb on - strand, within SO3560 at 3719.964 kb on - strand, within SO3560 at 3720.060 kb on + strand, within SO3560 at 3720.118 kb on + strand, within SO3560 at 3720.256 kb on + strand, within SO3560 at 3720.334 kb on - strand, within SO3560 at 3720.347 kb on - strand, within SO3560 at 3720.347 kb on - strand, within SO3560 at 3720.355 kb on - strand, within SO3560 at 3720.357 kb on + strand, within SO3560 at 3720.465 kb on + strand, within SO3560 at 3720.572 kb on + strand, within SO3560 at 3720.590 kb on - strand, within SO3560 at 3720.599 kb on - strand, within SO3560 at 3720.615 kb on + strand, within SO3560 at 3720.623 kb on - strand, within SO3560 at 3720.624 kb on + strand, within SO3560 at 3720.630 kb on + strand, within SO3560 at 3720.695 kb on - strand, within SO3560 at 3720.704 kb on + strand, within SO3560 at 3720.709 kb on + strand, within SO3560 at 3720.712 kb on - strand, within SO3560 at 3720.907 kb on + strand, within SO3560 at 3720.916 kb on - strand, within SO3560 at 3720.928 kb on - strand, within SO3560 at 3720.973 kb on - strand, within SO3560 at 3721.020 kb on + strand, within SO3560 at 3721.075 kb on + strand, within SO3560 at 3721.111 kb on - strand, within SO3560 at 3721.111 kb on - strand, within SO3560 at 3721.117 kb on - strand, within SO3560 at 3721.117 kb on - strand, within SO3560 at 3721.280 kb on - strand, within SO3560 at 3721.285 kb on - strand, within SO3560 at 3721.423 kb on - strand, within SO3560 at 3721.423 kb on - strand, within SO3560 at 3721.444 kb on + strand at 3721.449 kb on + strand at 3721.528 kb on - strand at 3721.542 kb on + strand at 3721.712 kb on + strand at 3721.753 kb on + strand at 3721.761 kb on - strand at 3721.761 kb on - strand at 3721.984 kb on + strand, within SO3561 at 3721.992 kb on - strand, within SO3561 at 3721.992 kb on - strand, within SO3561 at 3721.992 kb on - strand, within SO3561 at 3721.992 kb on - strand, within SO3561 at 3721.992 kb on - strand, within SO3561 at 3722.017 kb on + strand, within SO3561 at 3722.036 kb on + strand, within SO3561 at 3722.044 kb on - strand, within SO3561 at 3722.069 kb on + strand, within SO3561 at 3722.069 kb on + strand, within SO3561 at 3722.142 kb on + strand, within SO3561 at 3722.142 kb on + strand, within SO3561 at 3722.150 kb on - strand, within SO3561 at 3722.150 kb on - strand, within SO3561 at 3722.157 kb on - strand at 3722.158 kb on - strand, within SO3561 at 3722.185 kb on - strand, within SO3561 at 3722.234 kb on - strand, within SO3561 at 3722.515 kb on + strand at 3722.523 kb on - strand at 3722.677 kb on + strand at 3722.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 3,717,880 - gshA SO3559 0.52 -0.5 3,717,982 - gshA SO3559 0.58 -1.1 3,718,358 + gshA SO3559 0.81 -1.8 3,718,402 + gshA SO3559 0.83 +1.0 3,718,410 - gshA SO3559 0.84 -1.0 3,718,410 - gshA SO3559 0.84 +0.2 3,718,410 - gshA SO3559 0.84 -2.2 3,718,524 - -1.9 3,718,580 - +0.6 3,718,610 - +0.2 3,718,746 + +0.4 3,718,746 + -0.3 3,718,754 + -0.1 3,718,778 - +0.6 3,718,844 + +0.4 3,718,906 + -0.4 3,718,914 - -0.8 3,719,130 + -1.3 3,719,138 - -0.8 3,719,163 + -0.9 3,719,315 + SO3560 0.15 -1.1 3,719,337 + SO3560 0.16 +0.5 3,719,345 - SO3560 0.16 -1.2 3,719,424 - SO3560 0.19 -1.2 3,719,465 - SO3560 0.21 -0.7 3,719,470 - SO3560 0.21 -0.4 3,719,544 - SO3560 0.23 -1.2 3,719,585 + SO3560 0.25 -0.9 3,719,588 - SO3560 0.25 -0.9 3,719,632 + SO3560 0.26 -0.7 3,719,685 + SO3560 0.28 -1.4 3,719,693 - SO3560 0.29 -1.1 3,719,761 - SO3560 0.31 -1.1 3,719,854 - SO3560 0.34 -2.0 3,719,908 + SO3560 0.36 -0.2 3,719,956 + SO3560 0.38 -0.2 3,719,964 - SO3560 0.38 -0.9 3,719,964 - SO3560 0.38 -0.8 3,720,060 + SO3560 0.41 -1.1 3,720,118 + SO3560 0.43 -1.7 3,720,256 + SO3560 0.48 -0.6 3,720,334 - SO3560 0.51 -1.7 3,720,347 - SO3560 0.52 -0.4 3,720,347 - SO3560 0.52 -1.6 3,720,355 - SO3560 0.52 -1.5 3,720,357 + SO3560 0.52 -1.5 3,720,465 + SO3560 0.56 -0.3 3,720,572 + SO3560 0.59 -0.5 3,720,590 - SO3560 0.60 -2.1 3,720,599 - SO3560 0.60 -0.4 3,720,615 + SO3560 0.61 -0.1 3,720,623 - SO3560 0.61 -0.7 3,720,624 + SO3560 0.61 -1.5 3,720,630 + SO3560 0.61 -1.2 3,720,695 - SO3560 0.64 -1.8 3,720,704 + SO3560 0.64 -0.7 3,720,709 + SO3560 0.64 -0.8 3,720,712 - SO3560 0.64 -0.3 3,720,907 + SO3560 0.71 -1.2 3,720,916 - SO3560 0.71 -0.7 3,720,928 - SO3560 0.72 -0.8 3,720,973 - SO3560 0.73 -0.8 3,721,020 + SO3560 0.75 -0.9 3,721,075 + SO3560 0.77 -1.0 3,721,111 - SO3560 0.78 -0.5 3,721,111 - SO3560 0.78 -1.3 3,721,117 - SO3560 0.79 -1.4 3,721,117 - SO3560 0.79 -0.6 3,721,280 - SO3560 0.84 -1.1 3,721,285 - SO3560 0.84 -0.5 3,721,423 - SO3560 0.89 +0.1 3,721,423 - SO3560 0.89 -0.6 3,721,444 + -0.3 3,721,449 + -1.0 3,721,528 - -0.5 3,721,542 + +0.3 3,721,712 + -0.3 3,721,753 + -0.8 3,721,761 - -0.2 3,721,761 - +0.0 3,721,984 + SO3561 0.27 -0.1 3,721,992 - SO3561 0.29 +0.4 3,721,992 - SO3561 0.29 -0.0 3,721,992 - SO3561 0.29 -0.1 3,721,992 - SO3561 0.29 -0.6 3,721,992 - SO3561 0.29 +1.3 3,722,017 + SO3561 0.35 -0.5 3,722,036 + SO3561 0.39 -0.9 3,722,044 - SO3561 0.41 -0.9 3,722,069 + SO3561 0.47 -0.6 3,722,069 + SO3561 0.47 +0.1 3,722,142 + SO3561 0.64 -0.1 3,722,142 + SO3561 0.64 -0.1 3,722,150 - SO3561 0.66 +1.1 3,722,150 - SO3561 0.66 +0.2 3,722,157 - -0.9 3,722,158 - SO3561 0.68 -0.1 3,722,185 - SO3561 0.74 -0.4 3,722,234 - SO3561 0.85 +0.0 3,722,515 + +0.1 3,722,523 - +0.1 3,722,677 + -0.2 3,722,685 - +0.5
Or see this region's nucleotide sequence