Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and ihfA are separated by 3 nucleotides ihfA and SO2088 are separated by 96 nucleotides SO2088 and hypA are separated by 85 nucleotides hypA and hypE overlap by 1 nucleotides
SO2086: pheT - phenylalanyl-tRNA synthetase, beta subunit (NCBI ptt file), at 2,181,856 to 2,184,243
pheT
SO2087: ihfA - integration host factor, alpha subunit (NCBI ptt file), at 2,184,247 to 2,184,543
ihfA
SO2088: SO2088 - lipid A biosynthesis acyltransferase, putative (NCBI ptt file), at 2,184,640 to 2,185,578
SO2088
SO2089: hypA - hydrogenase expression/formation protein HypA (NCBI ptt file), at 2,185,664 to 2,186,020
hypA
SO2090: hypE - hydrogenase expression/formation protein HypE (NCBI ptt file), at 2,186,020 to 2,187,039
hypE
Position (kb)
2184
2185
2186 Strain fitness (log2 ratio)
-1
0
1 at 2184.948 kb on - strand, within SO2088 at 2184.950 kb on - strand, within SO2088 at 2185.166 kb on + strand, within SO2088 at 2185.359 kb on + strand, within SO2088 at 2185.719 kb on + strand, within hypA at 2185.769 kb on - strand, within hypA at 2185.871 kb on - strand, within hypA at 2185.900 kb on + strand, within hypA at 2186.151 kb on + strand, within hypE at 2186.267 kb on + strand, within hypE at 2186.274 kb on - strand, within hypE at 2186.454 kb on + strand, within hypE
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 2,184,948 - SO2088 0.33 -1.6 2,184,950 - SO2088 0.33 +1.1 2,185,166 + SO2088 0.56 -0.7 2,185,359 + SO2088 0.77 -1.0 2,185,719 + hypA SO2089 0.15 -0.2 2,185,769 - hypA SO2089 0.29 +1.2 2,185,871 - hypA SO2089 0.58 -1.0 2,185,900 + hypA SO2089 0.66 +0.4 2,186,151 + hypE SO2090 0.13 -0.8 2,186,267 + hypE SO2090 0.24 -0.9 2,186,274 - hypE SO2090 0.25 +0.0 2,186,454 + hypE SO2090 0.43 +0.3
Or see this region's nucleotide sequence