Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbD and SO1654 overlap by 26 nucleotides SO1654 and cysQ-2 are separated by 15 nucleotides cysQ-2 and SO1656 are separated by 62 nucleotides
SO1653: rfbD - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 1,741,411 to 1,742,397
rfbD
SO1654: SO1654 - hypothetical protein (NCBI ptt file), at 1,742,372 to 1,742,725
SO1654
SO1655: cysQ-2 - cysQ protein (NCBI ptt file), at 1,742,741 to 1,743,502
cysQ-2
SO1656: SO1656 - ROK family protein (NCBI ptt file), at 1,743,565 to 1,744,485
SO1656
Position (kb)
1742
1743
1744 Strain fitness (log2 ratio)
-1
0
1 at 1742.391 kb on + strand at 1742.392 kb on + strand at 1742.399 kb on - strand at 1742.407 kb on + strand at 1742.531 kb on + strand, within SO1654 at 1742.538 kb on - strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1743.129 kb on - strand, within cysQ-2 at 1743.338 kb on - strand, within cysQ-2 at 1743.422 kb on + strand, within cysQ-2 at 1743.428 kb on + strand at 1743.430 kb on - strand at 1743.565 kb on - strand at 1743.640 kb on + strand at 1743.690 kb on - strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.818 kb on - strand, within SO1656 at 1743.820 kb on + strand, within SO1656 at 1743.836 kb on + strand, within SO1656 at 1743.855 kb on - strand, within SO1656 at 1743.879 kb on + strand, within SO1656 at 1743.976 kb on - strand, within SO1656 at 1744.020 kb on - strand, within SO1656 at 1744.073 kb on - strand, within SO1656 at 1744.313 kb on - strand, within SO1656 at 1744.327 kb on - strand, within SO1656 at 1744.349 kb on - strand, within SO1656 at 1744.463 kb on - strand at 1744.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,742,391 + -0.1 1,742,392 + +1.2 1,742,399 - +0.9 1,742,407 + -0.1 1,742,531 + SO1654 0.45 +0.2 1,742,538 - SO1654 0.47 -0.6 1,742,566 + SO1654 0.55 -0.8 1,742,566 + SO1654 0.55 -0.2 1,742,566 + SO1654 0.55 -0.3 1,742,566 + SO1654 0.55 +1.0 1,742,566 + SO1654 0.55 +0.1 1,743,129 - cysQ-2 SO1655 0.51 -1.1 1,743,338 - cysQ-2 SO1655 0.78 -0.8 1,743,422 + cysQ-2 SO1655 0.89 -0.6 1,743,428 + -0.2 1,743,430 - -0.5 1,743,565 - -0.3 1,743,640 + +0.9 1,743,690 - SO1656 0.14 +0.8 1,743,776 + SO1656 0.23 +0.4 1,743,776 + SO1656 0.23 -0.4 1,743,776 + SO1656 0.23 -0.3 1,743,776 + SO1656 0.23 -1.0 1,743,776 + SO1656 0.23 +0.0 1,743,784 - SO1656 0.24 +0.5 1,743,784 - SO1656 0.24 +0.3 1,743,784 - SO1656 0.24 -0.2 1,743,818 - SO1656 0.27 +0.2 1,743,820 + SO1656 0.28 -0.9 1,743,836 + SO1656 0.29 +0.2 1,743,855 - SO1656 0.31 +0.1 1,743,879 + SO1656 0.34 -1.4 1,743,976 - SO1656 0.45 -0.3 1,744,020 - SO1656 0.49 +0.3 1,744,073 - SO1656 0.55 +0.3 1,744,313 - SO1656 0.81 +0.4 1,744,327 - SO1656 0.83 -0.5 1,744,349 - SO1656 0.85 +0.5 1,744,463 - -1.0 1,744,473 - +0.5
Or see this region's nucleotide sequence