Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dsbD and cutA overlap by 4 nucleotides cutA and fsxA are separated by 222 nucleotides fsxA and SO0699 are separated by 166 nucleotides
SO0696: dsbD - thiol:disulfide interchange protein DsbD (NCBI ptt file), at 714,174 to 716,006
dsbD
SO0697: cutA - periplasmic divalent cation tolerance protein CutA (NCBI ptt file), at 716,003 to 716,326
cutA
SO0698: fsxA - fxsA protein (NCBI ptt file), at 716,549 to 717,112
fsxA
SO0699: SO0699 - hypothetical protein (NCBI ptt file), at 717,279 to 717,647
SO0699
Position (kb) 716
717 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 715.185 kb on + strand, within dsbD at 715.193 kb on - strand, within dsbD at 715.253 kb on - strand, within dsbD at 715.257 kb on + strand, within dsbD at 715.288 kb on - strand, within dsbD at 715.376 kb on + strand, within dsbD at 715.390 kb on - strand, within dsbD at 715.447 kb on - strand, within dsbD at 715.491 kb on + strand, within dsbD at 715.630 kb on - strand, within dsbD at 715.630 kb on - strand, within dsbD at 715.634 kb on + strand, within dsbD at 715.642 kb on - strand, within dsbD at 715.642 kb on - strand, within dsbD at 715.766 kb on - strand, within dsbD at 715.884 kb on - strand at 715.982 kb on + strand at 715.990 kb on - strand at 716.008 kb on + strand at 716.013 kb on + strand at 716.037 kb on - strand, within cutA at 716.039 kb on + strand, within cutA at 716.122 kb on - strand, within cutA at 716.127 kb on - strand, within cutA at 716.273 kb on + strand, within cutA at 716.326 kb on - strand at 716.326 kb on - strand at 716.328 kb on + strand at 716.331 kb on - strand at 716.467 kb on - strand at 716.603 kb on + strand at 716.643 kb on + strand, within fsxA at 716.643 kb on + strand, within fsxA at 716.643 kb on + strand, within fsxA at 716.650 kb on - strand, within fsxA at 716.662 kb on - strand, within fsxA at 716.662 kb on - strand, within fsxA at 716.669 kb on + strand, within fsxA at 716.687 kb on - strand, within fsxA at 716.779 kb on - strand, within fsxA at 716.935 kb on + strand, within fsxA at 716.939 kb on + strand, within fsxA at 716.947 kb on - strand, within fsxA at 716.981 kb on - strand, within fsxA at 716.998 kb on + strand, within fsxA at 717.011 kb on - strand, within fsxA at 717.014 kb on - strand, within fsxA at 717.020 kb on + strand, within fsxA at 717.099 kb on + strand at 717.100 kb on + strand at 717.107 kb on - strand at 717.127 kb on + strand at 717.188 kb on + strand at 717.309 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 715,185 + dsbD SO0696 0.55 -1.0 715,193 - dsbD SO0696 0.56 -1.9 715,253 - dsbD SO0696 0.59 +0.0 715,257 + dsbD SO0696 0.59 -1.7 715,288 - dsbD SO0696 0.61 +0.6 715,376 + dsbD SO0696 0.66 -0.4 715,390 - dsbD SO0696 0.66 -2.2 715,447 - dsbD SO0696 0.69 -1.2 715,491 + dsbD SO0696 0.72 -0.2 715,630 - dsbD SO0696 0.79 -0.4 715,630 - dsbD SO0696 0.79 -2.8 715,634 + dsbD SO0696 0.80 -2.4 715,642 - dsbD SO0696 0.80 -2.5 715,642 - dsbD SO0696 0.80 -1.9 715,766 - dsbD SO0696 0.87 -0.6 715,884 - -1.7 715,982 + -0.9 715,990 - -0.7 716,008 + -0.4 716,013 + +0.2 716,037 - cutA SO0697 0.10 -0.8 716,039 + cutA SO0697 0.11 -2.1 716,122 - cutA SO0697 0.37 -0.3 716,127 - cutA SO0697 0.38 +0.0 716,273 + cutA SO0697 0.83 -2.1 716,326 - -0.1 716,326 - -1.2 716,328 + -1.2 716,331 - +0.0 716,467 - -0.1 716,603 + +0.0 716,643 + fsxA SO0698 0.17 -0.3 716,643 + fsxA SO0698 0.17 +0.0 716,643 + fsxA SO0698 0.17 +0.3 716,650 - fsxA SO0698 0.18 +0.7 716,662 - fsxA SO0698 0.20 -0.0 716,662 - fsxA SO0698 0.20 +2.0 716,669 + fsxA SO0698 0.21 -0.4 716,687 - fsxA SO0698 0.24 -0.5 716,779 - fsxA SO0698 0.41 +1.4 716,935 + fsxA SO0698 0.68 +0.4 716,939 + fsxA SO0698 0.69 +0.1 716,947 - fsxA SO0698 0.71 -0.4 716,981 - fsxA SO0698 0.77 -0.3 716,998 + fsxA SO0698 0.80 -0.3 717,011 - fsxA SO0698 0.82 -0.8 717,014 - fsxA SO0698 0.82 +0.0 717,020 + fsxA SO0698 0.84 +0.1 717,099 + -0.4 717,100 + -0.1 717,107 - -0.5 717,127 + +0.3 717,188 + -2.1 717,309 + -0.3
Or see this region's nucleotide sequence