Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petB and petC overlap by 1 nucleotides petC and sspA are separated by 96 nucleotides sspA and sspB overlap by 1 nucleotides sspB and pabA are separated by 154 nucleotides
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
SO0610: petC - ubiquinol-cytochrome c reductase, cytochrome c1 (NCBI ptt file), at 635,603 to 636,298
petC
SO0611: sspA - stringent starvation protein a (NCBI ptt file), at 636,395 to 637,024
sspA
SO0612: sspB - stringent starvation protein b (NCBI ptt file), at 637,024 to 637,461
sspB
SO0613: pabA - para-aminobenzoate synthase glutamine amidotransferase, component II (NCBI ptt file), at 637,616 to 638,191
pabA
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 635.701 kb on + strand, within petC at 636.333 kb on + strand at 636.346 kb on + strand at 636.500 kb on - strand, within sspA at 636.584 kb on - strand, within sspA at 636.717 kb on - strand, within sspA at 636.727 kb on - strand, within sspA at 636.845 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.898 kb on + strand, within sspA at 636.934 kb on - strand, within sspA at 637.064 kb on - strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.595 kb on - strand at 637.659 kb on - strand at 637.737 kb on + strand, within pabA at 637.742 kb on + strand, within pabA at 637.772 kb on - strand, within pabA at 637.857 kb on - strand, within pabA at 638.009 kb on + strand, within pabA
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 635,701 + petC SO0610 0.14 -0.9 636,333 + +0.1 636,346 + +0.0 636,500 - sspA SO0611 0.17 -0.2 636,584 - sspA SO0611 0.30 -1.5 636,717 - sspA SO0611 0.51 -0.7 636,727 - sspA SO0611 0.53 -3.5 636,845 + sspA SO0611 0.71 -0.7 636,878 + sspA SO0611 0.77 -1.4 636,878 + sspA SO0611 0.77 -1.0 636,898 + sspA SO0611 0.80 -0.8 636,934 - sspA SO0611 0.86 -0.4 637,064 - -0.1 637,587 + -0.4 637,587 + +0.1 637,587 + -0.8 637,587 + +0.3 637,587 + -0.1 637,595 - +0.9 637,659 - +0.1 637,737 + pabA SO0613 0.21 +0.2 637,742 + pabA SO0613 0.22 +0.5 637,772 - pabA SO0613 0.27 -0.6 637,857 - pabA SO0613 0.42 -0.6 638,009 + pabA SO0613 0.68 +0.3
Or see this region's nucleotide sequence