Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petB and petC overlap by 1 nucleotides petC and sspA are separated by 96 nucleotides sspA and sspB overlap by 1 nucleotides
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
SO0610: petC - ubiquinol-cytochrome c reductase, cytochrome c1 (NCBI ptt file), at 635,603 to 636,298
petC
SO0611: sspA - stringent starvation protein a (NCBI ptt file), at 636,395 to 637,024
sspA
SO0612: sspB - stringent starvation protein b (NCBI ptt file), at 637,024 to 637,461
sspB
Position (kb) 635
636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 634.631 kb on - strand, within petB at 634.631 kb on - strand, within petB at 634.717 kb on - strand, within petB at 634.929 kb on + strand, within petB at 634.983 kb on + strand, within petB at 635.012 kb on + strand, within petB at 635.032 kb on + strand, within petB at 635.123 kb on - strand, within petB at 635.190 kb on + strand, within petB at 635.222 kb on + strand, within petB at 635.239 kb on + strand, within petB at 635.242 kb on - strand, within petB at 635.360 kb on + strand, within petB at 635.360 kb on + strand, within petB at 635.701 kb on + strand, within petC at 636.333 kb on + strand at 636.346 kb on + strand at 636.500 kb on - strand, within sspA at 636.584 kb on - strand, within sspA at 636.717 kb on - strand, within sspA at 636.727 kb on - strand, within sspA at 636.845 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.898 kb on + strand, within sspA at 636.934 kb on - strand, within sspA at 637.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 634,631 - petB SO0609 0.20 -0.6 634,631 - petB SO0609 0.20 -1.1 634,717 - petB SO0609 0.27 -1.3 634,929 + petB SO0609 0.44 -2.3 634,983 + petB SO0609 0.49 -2.2 635,012 + petB SO0609 0.51 +0.5 635,032 + petB SO0609 0.53 -1.3 635,123 - petB SO0609 0.60 -0.9 635,190 + petB SO0609 0.66 -1.3 635,222 + petB SO0609 0.69 -0.6 635,239 + petB SO0609 0.70 -0.0 635,242 - petB SO0609 0.70 -1.8 635,360 + petB SO0609 0.80 -0.6 635,360 + petB SO0609 0.80 -2.5 635,701 + petC SO0610 0.14 -0.9 636,333 + +0.1 636,346 + +0.0 636,500 - sspA SO0611 0.17 -0.2 636,584 - sspA SO0611 0.30 -1.5 636,717 - sspA SO0611 0.51 -0.7 636,727 - sspA SO0611 0.53 -3.5 636,845 + sspA SO0611 0.71 -0.7 636,878 + sspA SO0611 0.77 -1.4 636,878 + sspA SO0611 0.77 -1.0 636,898 + sspA SO0611 0.80 -0.8 636,934 - sspA SO0611 0.86 -0.4 637,064 - -0.1
Or see this region's nucleotide sequence