Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0412 and SO0413 overlap by 8 nucleotides SO0413 and pilD are separated by 153 nucleotides pilD and SO0415 are separated by 165 nucleotides
SO0412: SO0412 - conserved hypothetical protein (NCBI ptt file), at 427,495 to 428,229
SO0412
SO0413: SO0413 - kinase, putative (NCBI ptt file), at 428,222 to 428,839
SO0413
SO0414: pilD - type 4 prepilin-like proteins leader peptide processing enzyme (NCBI ptt file), at 428,993 to 429,919
pilD
SO0415: SO0415 - type IV pilus biogenesis protein PilC (NCBI ptt file), at 430,085 to 431,356
SO0415
Position (kb)
428
429
430 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 428.053 kb on - strand, within SO0412 at 428.067 kb on + strand, within SO0412 at 428.094 kb on - strand, within SO0412 at 428.144 kb on + strand, within SO0412 at 428.147 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.198 kb on + strand at 428.198 kb on + strand at 428.225 kb on + strand at 428.898 kb on - strand at 428.987 kb on - strand at 428.988 kb on - strand at 429.070 kb on + strand at 429.105 kb on - strand, within pilD at 429.337 kb on + strand, within pilD at 429.355 kb on + strand, within pilD at 429.363 kb on - strand, within pilD at 429.511 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.598 kb on - strand, within pilD at 429.688 kb on - strand, within pilD at 429.993 kb on - strand at 430.106 kb on - strand at 430.126 kb on - strand at 430.143 kb on - strand at 430.217 kb on - strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.242 kb on + strand, within SO0415 at 430.268 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.541 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.552 kb on + strand, within SO0415 at 430.560 kb on - strand, within SO0415 at 430.562 kb on - strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.788 kb on + strand, within SO0415 at 430.841 kb on + strand, within SO0415 at 430.867 kb on + strand, within SO0415 at 430.896 kb on - strand, within SO0415
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 428,053 - SO0412 0.76 -0.0 428,067 + SO0412 0.78 +0.6 428,094 - SO0412 0.81 -0.3 428,144 + SO0412 0.88 -0.2 428,147 - SO0412 0.89 -0.1 428,152 - SO0412 0.89 -0.4 428,152 - SO0412 0.89 -0.5 428,198 + -0.7 428,198 + -1.5 428,225 + -0.2 428,898 - -0.1 428,987 - -0.5 428,988 - -0.7 429,070 + -2.8 429,105 - pilD SO0414 0.12 -1.6 429,337 + pilD SO0414 0.37 -0.9 429,355 + pilD SO0414 0.39 -2.1 429,363 - pilD SO0414 0.40 -0.1 429,511 - pilD SO0414 0.56 -2.5 429,588 - pilD SO0414 0.64 -2.9 429,588 - pilD SO0414 0.64 -2.1 429,598 - pilD SO0414 0.65 -1.9 429,688 - pilD SO0414 0.75 -1.3 429,993 - +0.2 430,106 - +0.9 430,126 - -0.6 430,143 - -0.0 430,217 - SO0415 0.10 -0.2 430,236 + SO0415 0.12 +0.1 430,236 + SO0415 0.12 +0.2 430,242 + SO0415 0.12 +0.0 430,268 + SO0415 0.14 -0.5 430,318 + SO0415 0.18 +0.1 430,318 + SO0415 0.18 +0.1 430,318 + SO0415 0.18 -1.3 430,326 - SO0415 0.19 -0.2 430,326 - SO0415 0.19 -0.3 430,326 - SO0415 0.19 -0.2 430,541 + SO0415 0.36 -0.4 430,542 + SO0415 0.36 -0.6 430,542 + SO0415 0.36 +0.1 430,550 - SO0415 0.37 +0.6 430,550 - SO0415 0.37 +0.0 430,550 - SO0415 0.37 -0.9 430,552 + SO0415 0.37 +0.1 430,560 - SO0415 0.37 -0.6 430,562 - SO0415 0.38 +0.1 430,745 + SO0415 0.52 +0.3 430,745 + SO0415 0.52 +0.1 430,788 + SO0415 0.55 +0.2 430,841 + SO0415 0.59 -0.2 430,867 + SO0415 0.61 -1.2 430,896 - SO0415 0.64 +0.1
Or see this region's nucleotide sequence