Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmC and ccmB are separated by 1 nucleotides ccmB and ccmA are separated by 11 nucleotides ccmA and scyA are separated by 325 nucleotides scyA and SO0265 are separated by 80 nucleotides
SO0261: ccmC - heme exporter protein CcmC (NCBI ptt file), at 261,223 to 261,969
ccmC
SO0262: ccmB - heme exporter protein CcmB (NCBI ptt file), at 261,971 to 262,657
ccmB
SO0263: ccmA - heme exporter protein CcmA (NCBI ptt file), at 262,669 to 263,319
ccmA
SO0264: scyA - cytochrome c (NCBI ptt file), at 263,645 to 263,944
scyA
SO0265: SO0265 - conserved hypothetical protein (NCBI ptt file), at 264,025 to 265,272
SO0265
Position (kb) 262
263
264 Strain fitness (log2 ratio) -2
-1
0
1 at 261.801 kb on - strand, within ccmC at 261.952 kb on - strand at 262.012 kb on + strand at 262.226 kb on + strand, within ccmB at 262.372 kb on + strand, within ccmB at 262.377 kb on + strand, within ccmB at 262.401 kb on - strand, within ccmB at 262.641 kb on - strand at 262.666 kb on + strand at 262.668 kb on - strand at 262.876 kb on - strand, within ccmA at 262.899 kb on - strand, within ccmA at 263.041 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.267 kb on + strand at 263.290 kb on - strand at 263.382 kb on + strand at 263.397 kb on + strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.430 kb on - strand at 263.431 kb on - strand at 263.499 kb on + strand at 263.509 kb on + strand at 263.582 kb on + strand at 263.587 kb on - strand at 263.593 kb on + strand at 263.613 kb on - strand at 263.636 kb on + strand at 263.659 kb on + strand at 263.734 kb on + strand, within scyA at 263.739 kb on + strand, within scyA at 263.752 kb on + strand, within scyA at 263.785 kb on + strand, within scyA at 263.937 kb on - strand at 264.094 kb on + strand at 264.147 kb on + strand at 264.147 kb on + strand at 264.197 kb on - strand, within SO0265 at 264.221 kb on + strand, within SO0265 at 264.246 kb on - strand, within SO0265 at 264.246 kb on - strand, within SO0265
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 261,801 - ccmC SO0261 0.77 -1.6 261,952 - +0.2 262,012 + -2.3 262,226 + ccmB SO0262 0.37 -0.5 262,372 + ccmB SO0262 0.58 -1.7 262,377 + ccmB SO0262 0.59 -0.9 262,401 - ccmB SO0262 0.63 -0.6 262,641 - -0.8 262,666 + -0.5 262,668 - -0.7 262,876 - ccmA SO0263 0.32 -0.5 262,899 - ccmA SO0263 0.35 -1.6 263,041 - ccmA SO0263 0.57 -2.3 263,120 - ccmA SO0263 0.69 +0.0 263,120 - ccmA SO0263 0.69 -0.5 263,267 + -2.3 263,290 - -1.2 263,382 + +0.2 263,397 + -0.1 263,406 - +0.8 263,406 - -0.6 263,406 - +0.1 263,430 - -0.0 263,431 - -0.8 263,499 + +0.0 263,509 + -0.0 263,582 + -1.7 263,587 - +0.8 263,593 + -1.1 263,613 - -0.4 263,636 + -2.4 263,659 + -1.1 263,734 + scyA SO0264 0.30 -0.7 263,739 + scyA SO0264 0.31 -1.0 263,752 + scyA SO0264 0.36 -0.4 263,785 + scyA SO0264 0.47 -1.0 263,937 - -1.9 264,094 + -0.4 264,147 + -0.7 264,147 + -1.0 264,197 - SO0265 0.14 +1.0 264,221 + SO0265 0.16 -0.5 264,246 - SO0265 0.18 -0.5 264,246 - SO0265 0.18 +0.0
Or see this region's nucleotide sequence