Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspL and gspM are separated by 1 nucleotides gspM and gspN are separated by 14 nucleotides gspN and SO0177 are separated by 59 nucleotides
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
SO0175: gspM - general secretion pathway protein M (NCBI ptt file), at 181,623 to 182,099
gspM
SO0176: gspN - general secretion pathway protein N (NCBI ptt file), at 182,114 to 182,875
gspN
SO0177: SO0177 - HAD-superfamily hydrolase, subfamily IA, variant 3 protein family (NCBI ptt file), at 182,935 to 183,597
SO0177
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL at 181.404 kb on - strand, within gspL at 181.416 kb on + strand, within gspL at 181.591 kb on - strand at 181.604 kb on + strand at 181.612 kb on - strand at 181.651 kb on + strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.659 kb on - strand at 181.734 kb on + strand, within gspM at 181.754 kb on - strand, within gspM at 181.798 kb on + strand, within gspM at 181.881 kb on - strand, within gspM at 182.067 kb on + strand at 182.081 kb on + strand at 182.104 kb on + strand at 182.149 kb on + strand at 182.149 kb on + strand at 182.152 kb on - strand at 182.171 kb on + strand at 182.229 kb on + strand, within gspN at 182.319 kb on + strand, within gspN at 182.404 kb on - strand, within gspN at 182.420 kb on + strand, within gspN at 182.425 kb on - strand, within gspN at 182.548 kb on + strand, within gspN at 182.626 kb on + strand, within gspN at 182.637 kb on + strand, within gspN at 182.645 kb on - strand, within gspN at 182.722 kb on + strand, within gspN at 182.748 kb on - strand, within gspN at 182.758 kb on + strand, within gspN at 182.766 kb on - strand, within gspN at 182.787 kb on - strand, within gspN at 182.827 kb on + strand at 182.869 kb on + strand at 182.966 kb on - strand at 183.038 kb on - strand, within SO0177 at 183.051 kb on - strand, within SO0177
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 180,628 - gspL SO0174 0.16 -2.2 180,628 - gspL SO0174 0.16 -1.4 180,697 - gspL SO0174 0.22 -0.9 180,910 - gspL SO0174 0.40 -1.3 180,920 + gspL SO0174 0.41 -2.2 180,932 + gspL SO0174 0.42 -2.2 181,035 + gspL SO0174 0.51 -3.1 181,078 - gspL SO0174 0.54 -0.6 181,147 - gspL SO0174 0.60 -1.2 181,225 - gspL SO0174 0.67 -1.8 181,281 - gspL SO0174 0.71 -1.0 181,311 - gspL SO0174 0.74 -1.4 181,364 + gspL SO0174 0.78 -1.9 181,364 + gspL SO0174 0.78 -1.2 181,372 - gspL SO0174 0.79 -1.4 181,372 - gspL SO0174 0.79 -2.0 181,404 - gspL SO0174 0.82 -3.2 181,416 + gspL SO0174 0.83 -2.3 181,591 - -2.4 181,604 + -0.4 181,612 - +0.8 181,651 + -1.7 181,659 - -1.0 181,659 - -2.0 181,659 - -2.2 181,734 + gspM SO0175 0.23 -3.0 181,754 - gspM SO0175 0.27 -1.3 181,798 + gspM SO0175 0.37 -3.3 181,881 - gspM SO0175 0.54 -2.8 182,067 + -1.2 182,081 + +0.0 182,104 + -0.1 182,149 + -0.5 182,149 + -1.3 182,152 - -1.0 182,171 + -0.9 182,229 + gspN SO0176 0.15 -0.7 182,319 + gspN SO0176 0.27 -2.1 182,404 - gspN SO0176 0.38 -2.0 182,420 + gspN SO0176 0.40 -0.8 182,425 - gspN SO0176 0.41 -0.3 182,548 + gspN SO0176 0.57 -1.0 182,626 + gspN SO0176 0.67 -2.9 182,637 + gspN SO0176 0.69 -1.8 182,645 - gspN SO0176 0.70 -1.1 182,722 + gspN SO0176 0.80 -0.4 182,748 - gspN SO0176 0.83 -0.7 182,758 + gspN SO0176 0.85 -0.7 182,766 - gspN SO0176 0.86 -0.4 182,787 - gspN SO0176 0.88 -1.5 182,827 + -0.5 182,869 + -0.1 182,966 - -1.3 183,038 - SO0177 0.16 +0.3 183,051 - SO0177 0.17 -1.1
Or see this region's nucleotide sequence