Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspI and gspJ overlap by 20 nucleotides gspJ and gspK overlap by 4 nucleotides gspK and gspL are separated by 48 nucleotides
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
Position (kb) 179
180
181 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ at 179.303 kb on - strand, within gspJ at 179.394 kb on + strand at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK at 179.645 kb on + strand, within gspK at 179.785 kb on + strand, within gspK at 179.849 kb on - strand, within gspK at 179.892 kb on + strand, within gspK at 180.055 kb on + strand, within gspK at 180.102 kb on - strand, within gspK at 180.209 kb on - strand, within gspK at 180.410 kb on + strand at 180.418 kb on - strand at 180.424 kb on + strand at 180.476 kb on - strand at 180.493 kb on + strand at 180.538 kb on + strand at 180.546 kb on - strand at 180.546 kb on - strand at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 178,440 + gspI SO0171 0.45 -2.5 178,516 - gspI SO0171 0.66 -2.9 178,605 - gspI SO0171 0.90 +0.1 178,654 + -0.4 178,654 + -1.7 178,654 + -1.9 178,654 + -1.6 178,654 + -1.7 178,654 + -2.4 178,662 - -3.3 178,662 - -1.8 178,700 + gspJ SO0172 0.10 -1.2 178,730 - gspJ SO0172 0.14 -2.3 178,742 - gspJ SO0172 0.15 -2.4 178,744 + gspJ SO0172 0.16 -1.2 178,816 + gspJ SO0172 0.25 -3.2 178,824 - gspJ SO0172 0.26 -2.2 178,849 - gspJ SO0172 0.29 -2.8 178,850 + gspJ SO0172 0.30 -3.9 178,867 + gspJ SO0172 0.32 +0.3 178,943 + gspJ SO0172 0.42 -1.5 178,943 + gspJ SO0172 0.42 -3.0 178,950 - gspJ SO0172 0.43 -2.0 178,951 - gspJ SO0172 0.43 -1.8 179,018 - gspJ SO0172 0.51 -2.9 179,151 + gspJ SO0172 0.69 -2.7 179,157 + gspJ SO0172 0.70 -0.2 179,220 - gspJ SO0172 0.78 -1.0 179,273 + gspJ SO0172 0.85 +0.8 179,303 - gspJ SO0172 0.89 -0.3 179,394 + -1.3 179,436 - -1.8 179,544 - gspK SO0173 0.16 -1.5 179,635 + gspK SO0173 0.25 -1.8 179,645 + gspK SO0173 0.26 -1.3 179,785 + gspK SO0173 0.40 -1.2 179,849 - gspK SO0173 0.46 -3.2 179,892 + gspK SO0173 0.51 -2.0 180,055 + gspK SO0173 0.67 -1.2 180,102 - gspK SO0173 0.72 -2.3 180,209 - gspK SO0173 0.82 -0.9 180,410 + -0.4 180,418 - -1.8 180,424 + -0.3 180,476 - -3.6 180,493 + -2.7 180,538 + -1.5 180,546 - -0.0 180,546 - -0.2 180,569 - gspL SO0174 0.11 -1.3 180,569 - gspL SO0174 0.11 -1.6 180,569 - gspL SO0174 0.11 -1.2 180,628 - gspL SO0174 0.16 -2.2 180,628 - gspL SO0174 0.16 -1.4 180,697 - gspL SO0174 0.22 -0.9 180,910 - gspL SO0174 0.40 -1.3 180,920 + gspL SO0174 0.41 -2.2 180,932 + gspL SO0174 0.42 -2.2 181,035 + gspL SO0174 0.51 -3.1 181,078 - gspL SO0174 0.54 -0.6 181,147 - gspL SO0174 0.60 -1.2 181,225 - gspL SO0174 0.67 -1.8 181,281 - gspL SO0174 0.71 -1.0 181,311 - gspL SO0174 0.74 -1.4 181,364 + gspL SO0174 0.78 -1.9 181,364 + gspL SO0174 0.78 -1.2 181,372 - gspL SO0174 0.79 -1.4 181,372 - gspL SO0174 0.79 -2.0
Or see this region's nucleotide sequence