Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hslO and hslR are separated by 25 nucleotides hslR and gspC are separated by 251 nucleotides gspC and gspD are separated by 24 nucleotides
SO0163: hslO - chaperonin HslO (NCBI ptt file), at 169,814 to 170,674
hslO
SO0164: hslR - heat shock protein 15 (NCBI ptt file), at 170,700 to 171,098
hslR
SO0165: gspC - general secretion pathway protein C (NCBI ptt file), at 171,350 to 172,276
gspC
SO0166: gspD - general secretion pathway protein D (NCBI ptt file), at 172,301 to 174,415
gspD
Position (kb) 171
172
173 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 170.369 kb on + strand, within hslO at 170.377 kb on - strand, within hslO at 170.549 kb on + strand, within hslO at 170.678 kb on - strand at 170.772 kb on - strand, within hslR at 170.984 kb on - strand, within hslR at 170.984 kb on - strand, within hslR at 171.080 kb on + strand at 171.088 kb on - strand at 171.139 kb on + strand at 171.163 kb on + strand at 171.171 kb on + strand at 171.204 kb on - strand at 171.281 kb on + strand at 171.305 kb on - strand at 171.329 kb on + strand at 171.361 kb on + strand at 171.387 kb on - strand at 171.453 kb on - strand, within gspC at 171.513 kb on - strand, within gspC at 171.518 kb on - strand, within gspC at 171.520 kb on + strand, within gspC at 171.528 kb on - strand, within gspC at 171.537 kb on - strand, within gspC at 171.538 kb on - strand, within gspC at 171.626 kb on + strand, within gspC at 171.634 kb on - strand, within gspC at 171.655 kb on - strand, within gspC at 171.663 kb on - strand, within gspC at 171.812 kb on + strand, within gspC at 171.834 kb on + strand, within gspC at 171.867 kb on - strand, within gspC at 171.931 kb on - strand, within gspC at 171.963 kb on + strand, within gspC at 172.073 kb on - strand, within gspC at 172.096 kb on - strand, within gspC at 172.096 kb on - strand, within gspC at 172.130 kb on - strand, within gspC at 172.315 kb on + strand at 172.401 kb on + strand at 172.509 kb on + strand at 172.514 kb on - strand, within gspD at 172.516 kb on + strand, within gspD at 172.562 kb on + strand, within gspD at 172.575 kb on - strand, within gspD at 172.667 kb on + strand, within gspD at 172.667 kb on + strand, within gspD at 172.667 kb on + strand, within gspD at 172.675 kb on - strand, within gspD at 172.700 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.718 kb on - strand, within gspD at 172.741 kb on - strand, within gspD at 172.775 kb on - strand, within gspD at 172.791 kb on + strand, within gspD at 172.799 kb on - strand, within gspD at 172.812 kb on - strand, within gspD at 172.940 kb on + strand, within gspD at 172.948 kb on - strand, within gspD at 172.948 kb on - strand, within gspD at 172.948 kb on - strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.019 kb on - strand, within gspD at 173.019 kb on - strand, within gspD at 173.019 kb on - strand, within gspD at 173.055 kb on + strand, within gspD at 173.087 kb on + strand, within gspD at 173.087 kb on + strand, within gspD at 173.092 kb on + strand, within gspD at 173.101 kb on + strand, within gspD at 173.107 kb on + strand, within gspD at 173.129 kb on - strand, within gspD at 173.146 kb on - strand, within gspD at 173.146 kb on - strand, within gspD at 173.156 kb on + strand, within gspD at 173.156 kb on - strand, within gspD at 173.174 kb on - strand, within gspD
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 170,369 + hslO SO0163 0.64 -0.5 170,377 - hslO SO0163 0.65 -0.1 170,549 + hslO SO0163 0.85 -0.4 170,678 - -0.1 170,772 - hslR SO0164 0.18 +0.1 170,984 - hslR SO0164 0.71 -0.3 170,984 - hslR SO0164 0.71 -0.3 171,080 + +0.1 171,088 - +0.2 171,139 + +0.0 171,163 + -0.4 171,171 + +0.3 171,204 - -0.4 171,281 + -0.1 171,305 - -1.3 171,329 + +0.5 171,361 + -1.3 171,387 - -1.9 171,453 - gspC SO0165 0.11 -1.8 171,513 - gspC SO0165 0.18 -1.0 171,518 - gspC SO0165 0.18 -1.0 171,520 + gspC SO0165 0.18 -1.4 171,528 - gspC SO0165 0.19 -3.3 171,537 - gspC SO0165 0.20 -1.5 171,538 - gspC SO0165 0.20 -0.9 171,626 + gspC SO0165 0.30 -1.4 171,634 - gspC SO0165 0.31 -2.7 171,655 - gspC SO0165 0.33 -1.5 171,663 - gspC SO0165 0.34 -0.9 171,812 + gspC SO0165 0.50 -1.8 171,834 + gspC SO0165 0.52 -0.9 171,867 - gspC SO0165 0.56 -2.8 171,931 - gspC SO0165 0.63 -2.3 171,963 + gspC SO0165 0.66 -1.6 172,073 - gspC SO0165 0.78 -2.2 172,096 - gspC SO0165 0.80 -1.7 172,096 - gspC SO0165 0.80 +0.0 172,130 - gspC SO0165 0.84 -2.2 172,315 + -2.8 172,401 + -2.4 172,509 + -1.5 172,514 - gspD SO0166 0.10 -1.8 172,516 + gspD SO0166 0.10 -2.9 172,562 + gspD SO0166 0.12 -1.6 172,575 - gspD SO0166 0.13 -3.0 172,667 + gspD SO0166 0.17 -2.2 172,667 + gspD SO0166 0.17 -2.0 172,667 + gspD SO0166 0.17 -4.0 172,675 - gspD SO0166 0.18 -2.6 172,700 + gspD SO0166 0.19 -2.6 172,705 + gspD SO0166 0.19 -1.6 172,705 + gspD SO0166 0.19 -0.2 172,705 + gspD SO0166 0.19 -2.0 172,705 + gspD SO0166 0.19 -1.6 172,705 + gspD SO0166 0.19 +0.1 172,705 + gspD SO0166 0.19 -1.0 172,705 + gspD SO0166 0.19 -1.3 172,713 - gspD SO0166 0.19 -0.8 172,713 - gspD SO0166 0.19 -1.2 172,713 - gspD SO0166 0.19 -2.5 172,713 - gspD SO0166 0.19 -2.3 172,713 - gspD SO0166 0.19 -0.7 172,713 - gspD SO0166 0.19 -2.2 172,713 - gspD SO0166 0.19 -2.2 172,718 - gspD SO0166 0.20 -1.8 172,741 - gspD SO0166 0.21 -1.1 172,775 - gspD SO0166 0.22 -3.1 172,791 + gspD SO0166 0.23 -0.4 172,799 - gspD SO0166 0.24 -1.8 172,812 - gspD SO0166 0.24 -0.1 172,940 + gspD SO0166 0.30 -2.7 172,948 - gspD SO0166 0.31 -1.5 172,948 - gspD SO0166 0.31 -1.4 172,948 - gspD SO0166 0.31 -3.1 173,011 + gspD SO0166 0.34 -2.3 173,011 + gspD SO0166 0.34 -2.7 173,011 + gspD SO0166 0.34 -3.3 173,011 + gspD SO0166 0.34 -1.5 173,019 - gspD SO0166 0.34 -1.0 173,019 - gspD SO0166 0.34 -2.8 173,019 - gspD SO0166 0.34 -0.7 173,055 + gspD SO0166 0.36 -2.1 173,087 + gspD SO0166 0.37 -2.5 173,087 + gspD SO0166 0.37 -2.1 173,092 + gspD SO0166 0.37 -1.0 173,101 + gspD SO0166 0.38 -3.0 173,107 + gspD SO0166 0.38 -0.1 173,129 - gspD SO0166 0.39 -0.9 173,146 - gspD SO0166 0.40 -1.1 173,146 - gspD SO0166 0.40 -1.9 173,156 + gspD SO0166 0.40 -0.9 173,156 - gspD SO0166 0.40 -2.1 173,174 - gspD SO0166 0.41 +0.5
Or see this region's nucleotide sequence