Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09980 and PGA1_c09990 are separated by 93 nucleotides PGA1_c09990 and PGA1_c10000 overlap by 1 nucleotides PGA1_c10000 and PGA1_c10010 are separated by 246 nucleotides
PGA1_c09980: PGA1_c09980 - kynureninase KynU, at 1,031,352 to 1,032,545
_c09980
PGA1_c09990: PGA1_c09990 - Uncharacterized protein conserved in bacteria, at 1,032,639 to 1,033,037
_c09990
PGA1_c10000: PGA1_c10000 - short chain dehydrogenase / reductase, at 1,033,037 to 1,033,789
_c10000
PGA1_c10010: PGA1_c10010 - putative protein SoxH, at 1,034,036 to 1,034,989
_c10010
Position (kb)
1032
1033
1034 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1031.660 kb on + strand, within PGA1_c09980 at 1031.698 kb on - strand, within PGA1_c09980 at 1031.718 kb on + strand, within PGA1_c09980 at 1031.726 kb on - strand, within PGA1_c09980 at 1031.726 kb on - strand, within PGA1_c09980 at 1031.726 kb on - strand, within PGA1_c09980 at 1031.883 kb on - strand, within PGA1_c09980 at 1031.888 kb on - strand, within PGA1_c09980 at 1031.888 kb on - strand, within PGA1_c09980 at 1031.952 kb on - strand, within PGA1_c09980 at 1031.985 kb on - strand, within PGA1_c09980 at 1032.019 kb on + strand, within PGA1_c09980 at 1032.019 kb on + strand, within PGA1_c09980 at 1032.019 kb on + strand, within PGA1_c09980 at 1032.040 kb on + strand, within PGA1_c09980 at 1032.061 kb on + strand, within PGA1_c09980 at 1032.076 kb on + strand, within PGA1_c09980 at 1032.095 kb on + strand, within PGA1_c09980 at 1032.113 kb on + strand, within PGA1_c09980 at 1032.123 kb on + strand, within PGA1_c09980 at 1032.149 kb on + strand, within PGA1_c09980 at 1032.163 kb on - strand, within PGA1_c09980 at 1032.180 kb on + strand, within PGA1_c09980 at 1032.180 kb on + strand, within PGA1_c09980 at 1032.188 kb on - strand, within PGA1_c09980 at 1032.279 kb on - strand, within PGA1_c09980 at 1032.325 kb on + strand, within PGA1_c09980 at 1032.333 kb on - strand, within PGA1_c09980 at 1032.387 kb on + strand, within PGA1_c09980 at 1032.387 kb on + strand, within PGA1_c09980 at 1032.390 kb on - strand, within PGA1_c09980 at 1032.390 kb on - strand, within PGA1_c09980 at 1032.405 kb on - strand, within PGA1_c09980 at 1032.487 kb on + strand at 1032.487 kb on + strand at 1032.543 kb on + strand at 1032.543 kb on + strand at 1032.545 kb on + strand at 1032.680 kb on + strand, within PGA1_c09990 at 1032.735 kb on + strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.745 kb on + strand, within PGA1_c09990 at 1032.750 kb on + strand, within PGA1_c09990 at 1032.779 kb on + strand, within PGA1_c09990 at 1032.959 kb on + strand, within PGA1_c09990 at 1032.991 kb on - strand, within PGA1_c09990 at 1032.991 kb on - strand, within PGA1_c09990 at 1033.033 kb on - strand at 1033.040 kb on + strand at 1033.040 kb on + strand at 1033.089 kb on - strand at 1033.144 kb on - strand, within PGA1_c10000 at 1033.165 kb on + strand, within PGA1_c10000 at 1033.197 kb on + strand, within PGA1_c10000 at 1033.207 kb on + strand, within PGA1_c10000 at 1033.281 kb on + strand, within PGA1_c10000 at 1033.283 kb on - strand, within PGA1_c10000 at 1033.321 kb on - strand, within PGA1_c10000 at 1033.335 kb on - strand, within PGA1_c10000 at 1033.378 kb on - strand, within PGA1_c10000 at 1033.406 kb on + strand, within PGA1_c10000 at 1033.438 kb on + strand, within PGA1_c10000 at 1033.514 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.623 kb on + strand, within PGA1_c10000 at 1033.669 kb on + strand, within PGA1_c10000 at 1033.677 kb on - strand, within PGA1_c10000 at 1033.713 kb on + strand, within PGA1_c10000 at 1033.725 kb on + strand at 1033.799 kb on - strand at 1033.888 kb on - strand at 1033.907 kb on + strand at 1033.921 kb on - strand at 1033.956 kb on - strand at 1034.018 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 1,031,660 + PGA1_c09980 0.26 +0.7 1,031,698 - PGA1_c09980 0.29 +0.5 1,031,718 + PGA1_c09980 0.31 -0.6 1,031,726 - PGA1_c09980 0.31 +0.0 1,031,726 - PGA1_c09980 0.31 -0.0 1,031,726 - PGA1_c09980 0.31 -2.5 1,031,883 - PGA1_c09980 0.44 -0.1 1,031,888 - PGA1_c09980 0.45 -0.3 1,031,888 - PGA1_c09980 0.45 +0.1 1,031,952 - PGA1_c09980 0.50 -0.5 1,031,985 - PGA1_c09980 0.53 +0.1 1,032,019 + PGA1_c09980 0.56 +0.4 1,032,019 + PGA1_c09980 0.56 +0.3 1,032,019 + PGA1_c09980 0.56 -0.1 1,032,040 + PGA1_c09980 0.58 +0.6 1,032,061 + PGA1_c09980 0.59 -0.1 1,032,076 + PGA1_c09980 0.61 -0.9 1,032,095 + PGA1_c09980 0.62 +0.9 1,032,113 + PGA1_c09980 0.64 -2.4 1,032,123 + PGA1_c09980 0.65 +0.1 1,032,149 + PGA1_c09980 0.67 -0.5 1,032,163 - PGA1_c09980 0.68 +2.0 1,032,180 + PGA1_c09980 0.69 -1.5 1,032,180 + PGA1_c09980 0.69 -0.6 1,032,188 - PGA1_c09980 0.70 -1.0 1,032,279 - PGA1_c09980 0.78 +1.1 1,032,325 + PGA1_c09980 0.81 +0.2 1,032,333 - PGA1_c09980 0.82 -0.4 1,032,387 + PGA1_c09980 0.87 +0.7 1,032,387 + PGA1_c09980 0.87 -0.9 1,032,390 - PGA1_c09980 0.87 -2.7 1,032,390 - PGA1_c09980 0.87 +1.2 1,032,405 - PGA1_c09980 0.88 -0.0 1,032,487 + -0.9 1,032,487 + -0.8 1,032,543 + +0.6 1,032,543 + +0.5 1,032,545 + -2.0 1,032,680 + PGA1_c09990 0.10 -2.1 1,032,735 + PGA1_c09990 0.24 -0.4 1,032,744 - PGA1_c09990 0.26 +0.9 1,032,744 - PGA1_c09990 0.26 +0.1 1,032,744 - PGA1_c09990 0.26 -1.0 1,032,745 + PGA1_c09990 0.27 -0.8 1,032,750 + PGA1_c09990 0.28 +1.0 1,032,779 + PGA1_c09990 0.35 -0.0 1,032,959 + PGA1_c09990 0.80 +0.4 1,032,991 - PGA1_c09990 0.88 -1.0 1,032,991 - PGA1_c09990 0.88 +1.7 1,033,033 - -1.5 1,033,040 + +0.7 1,033,040 + +0.2 1,033,089 - +0.2 1,033,144 - PGA1_c10000 0.14 -0.0 1,033,165 + PGA1_c10000 0.17 -0.2 1,033,197 + PGA1_c10000 0.21 -2.6 1,033,207 + PGA1_c10000 0.23 +0.2 1,033,281 + PGA1_c10000 0.32 +0.2 1,033,283 - PGA1_c10000 0.33 +0.1 1,033,321 - PGA1_c10000 0.38 -2.2 1,033,335 - PGA1_c10000 0.40 -0.4 1,033,378 - PGA1_c10000 0.45 -1.0 1,033,406 + PGA1_c10000 0.49 +0.3 1,033,438 + PGA1_c10000 0.53 +2.0 1,033,514 - PGA1_c10000 0.63 -1.8 1,033,526 - PGA1_c10000 0.65 -1.2 1,033,526 - PGA1_c10000 0.65 +0.5 1,033,526 - PGA1_c10000 0.65 -1.4 1,033,526 - PGA1_c10000 0.65 -0.2 1,033,561 - PGA1_c10000 0.70 -0.6 1,033,561 - PGA1_c10000 0.70 -0.4 1,033,561 - PGA1_c10000 0.70 +0.5 1,033,561 - PGA1_c10000 0.70 +0.2 1,033,623 + PGA1_c10000 0.78 +0.4 1,033,669 + PGA1_c10000 0.84 -1.8 1,033,677 - PGA1_c10000 0.85 -0.2 1,033,713 + PGA1_c10000 0.90 -0.3 1,033,725 + +1.2 1,033,799 - +1.6 1,033,888 - -0.3 1,033,907 + -0.8 1,033,921 - -0.4 1,033,956 - +0.6 1,034,018 - +1.0
Or see this region's nucleotide sequence