Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17500 and PGA1_c17510 are separated by 214 nucleotides PGA1_c17510 and PGA1_c17520 overlap by 8 nucleotides PGA1_c17520 and PGA1_c17530 are separated by 181 nucleotides
PGA1_c17500: PGA1_c17500 - Protein of unknown function (DUF3179)., at 1,834,985 to 1,835,992
_c17500
PGA1_c17510: PGA1_c17510 - putative peptidyl-prolyl cis-trans isomerases (cyclophilin), at 1,836,207 to 1,836,758
_c17510
PGA1_c17520: PGA1_c17520 - putative peptidyl-prolyl cis-trans isomerase A, at 1,836,751 to 1,837,257
_c17520
PGA1_c17530: PGA1_c17530 - phosphoglycerate kinase Pgk, at 1,837,439 to 1,838,629
_c17530
Position (kb)
1836
1837 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1835.208 kb on + strand, within PGA1_c17500 at 1835.263 kb on - strand, within PGA1_c17500 at 1835.301 kb on - strand, within PGA1_c17500 at 1835.322 kb on - strand, within PGA1_c17500 at 1835.463 kb on + strand, within PGA1_c17500 at 1835.520 kb on - strand, within PGA1_c17500 at 1835.636 kb on - strand, within PGA1_c17500 at 1835.659 kb on + strand, within PGA1_c17500 at 1835.672 kb on - strand, within PGA1_c17500 at 1835.731 kb on + strand, within PGA1_c17500 at 1835.739 kb on + strand, within PGA1_c17500 at 1835.739 kb on + strand, within PGA1_c17500 at 1835.836 kb on - strand, within PGA1_c17500 at 1835.892 kb on + strand at 1835.999 kb on + strand at 1836.000 kb on - strand at 1836.005 kb on - strand at 1836.009 kb on - strand at 1836.034 kb on - strand at 1836.083 kb on + strand at 1836.095 kb on + strand at 1836.120 kb on + strand at 1836.168 kb on + strand at 1836.212 kb on + strand at 1836.307 kb on - strand, within PGA1_c17510 at 1836.321 kb on + strand, within PGA1_c17510 at 1836.338 kb on - strand, within PGA1_c17510 at 1836.473 kb on + strand, within PGA1_c17510 at 1836.491 kb on - strand, within PGA1_c17510 at 1836.532 kb on + strand, within PGA1_c17510 at 1836.567 kb on + strand, within PGA1_c17510 at 1836.575 kb on - strand, within PGA1_c17510 at 1836.615 kb on - strand, within PGA1_c17510 at 1836.615 kb on - strand, within PGA1_c17510 at 1836.615 kb on - strand, within PGA1_c17510 at 1836.703 kb on + strand at 1836.721 kb on - strand at 1836.768 kb on + strand at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.853 kb on + strand, within PGA1_c17520 at 1836.860 kb on + strand, within PGA1_c17520 at 1836.861 kb on - strand, within PGA1_c17520 at 1836.861 kb on - strand, within PGA1_c17520 at 1836.861 kb on - strand, within PGA1_c17520 at 1837.005 kb on + strand, within PGA1_c17520 at 1837.005 kb on + strand, within PGA1_c17520 at 1837.035 kb on + strand, within PGA1_c17520 at 1837.162 kb on + strand, within PGA1_c17520 at 1837.162 kb on + strand, within PGA1_c17520 at 1837.162 kb on + strand, within PGA1_c17520 at 1837.162 kb on + strand, within PGA1_c17520 at 1837.170 kb on - strand, within PGA1_c17520 at 1837.170 kb on - strand, within PGA1_c17520 at 1837.170 kb on - strand, within PGA1_c17520 at 1837.175 kb on + strand, within PGA1_c17520 at 1837.261 kb on - strand at 1837.264 kb on + strand at 1837.266 kb on + strand at 1837.272 kb on - strand at 1837.298 kb on - strand at 1837.298 kb on - strand at 1837.298 kb on - strand at 1837.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 1,835,208 + PGA1_c17500 0.22 -0.4 1,835,263 - PGA1_c17500 0.28 +2.0 1,835,301 - PGA1_c17500 0.31 -0.6 1,835,322 - PGA1_c17500 0.33 +1.1 1,835,463 + PGA1_c17500 0.47 +2.0 1,835,520 - PGA1_c17500 0.53 -0.6 1,835,636 - PGA1_c17500 0.65 -0.2 1,835,659 + PGA1_c17500 0.67 +0.8 1,835,672 - PGA1_c17500 0.68 -1.2 1,835,731 + PGA1_c17500 0.74 +0.8 1,835,739 + PGA1_c17500 0.75 -0.8 1,835,739 + PGA1_c17500 0.75 +2.1 1,835,836 - PGA1_c17500 0.84 +1.4 1,835,892 + -0.8 1,835,999 + +1.1 1,836,000 - +0.2 1,836,005 - +2.4 1,836,009 - -0.3 1,836,034 - -1.5 1,836,083 + +0.5 1,836,095 + -0.5 1,836,120 + -1.5 1,836,168 + -1.8 1,836,212 + +1.8 1,836,307 - PGA1_c17510 0.18 -1.0 1,836,321 + PGA1_c17510 0.21 +0.5 1,836,338 - PGA1_c17510 0.24 -0.8 1,836,473 + PGA1_c17510 0.48 -1.5 1,836,491 - PGA1_c17510 0.51 -3.2 1,836,532 + PGA1_c17510 0.59 -0.8 1,836,567 + PGA1_c17510 0.65 +1.2 1,836,575 - PGA1_c17510 0.67 -2.2 1,836,615 - PGA1_c17510 0.74 -0.2 1,836,615 - PGA1_c17510 0.74 -0.5 1,836,615 - PGA1_c17510 0.74 +0.1 1,836,703 + -0.4 1,836,721 - -0.5 1,836,768 + -0.6 1,836,853 + PGA1_c17520 0.20 -0.2 1,836,853 + PGA1_c17520 0.20 -1.2 1,836,853 + PGA1_c17520 0.20 +2.4 1,836,853 + PGA1_c17520 0.20 -0.8 1,836,853 + PGA1_c17520 0.20 -0.8 1,836,853 + PGA1_c17520 0.20 +0.8 1,836,853 + PGA1_c17520 0.20 -0.2 1,836,860 + PGA1_c17520 0.21 -0.5 1,836,861 - PGA1_c17520 0.22 -0.2 1,836,861 - PGA1_c17520 0.22 +0.8 1,836,861 - PGA1_c17520 0.22 -0.5 1,837,005 + PGA1_c17520 0.50 +1.2 1,837,005 + PGA1_c17520 0.50 +0.8 1,837,035 + PGA1_c17520 0.56 +0.2 1,837,162 + PGA1_c17520 0.81 -1.2 1,837,162 + PGA1_c17520 0.81 +0.1 1,837,162 + PGA1_c17520 0.81 +0.8 1,837,162 + PGA1_c17520 0.81 -0.8 1,837,170 - PGA1_c17520 0.83 +2.4 1,837,170 - PGA1_c17520 0.83 -0.2 1,837,170 - PGA1_c17520 0.83 -0.2 1,837,175 + PGA1_c17520 0.84 +0.2 1,837,261 - -1.8 1,837,264 + +1.8 1,837,266 + -0.0 1,837,272 - +0.0 1,837,298 - +2.4 1,837,298 - -2.5 1,837,298 - +0.6 1,837,329 - +1.1
Or see this region's nucleotide sequence