Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c15110 and PGA1_c15120 are separated by 2 nucleotides PGA1_c15120 and PGA1_c15130 are separated by 97 nucleotides PGA1_c15130 and PGA1_c15140 are separated by 130 nucleotides
PGA1_c15110: PGA1_c15110 - heme A synthase CtaA, at 1,569,977 to 1,571,125
_c15110
PGA1_c15120: PGA1_c15120 - thermostable carboxypeptidase 1, at 1,571,128 to 1,572,597
_c15120
PGA1_c15130: PGA1_c15130 - hypothetical protein, at 1,572,695 to 1,573,045
_c15130
PGA1_c15140: PGA1_c15140 - DNA gyrase subunit GyrA, at 1,573,176 to 1,575,902
_c15140
Position (kb)
1571
1572
1573 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1571.089 kb on + strand at 1571.168 kb on - strand at 1571.179 kb on + strand at 1571.343 kb on + strand, within PGA1_c15120 at 1571.378 kb on + strand, within PGA1_c15120 at 1571.398 kb on - strand, within PGA1_c15120 at 1571.435 kb on + strand, within PGA1_c15120 at 1571.549 kb on - strand, within PGA1_c15120 at 1571.557 kb on + strand, within PGA1_c15120 at 1571.593 kb on + strand, within PGA1_c15120 at 1571.603 kb on - strand, within PGA1_c15120 at 1571.625 kb on + strand, within PGA1_c15120 at 1571.648 kb on + strand, within PGA1_c15120 at 1571.648 kb on + strand, within PGA1_c15120 at 1571.656 kb on - strand, within PGA1_c15120 at 1571.736 kb on - strand, within PGA1_c15120 at 1571.827 kb on - strand, within PGA1_c15120 at 1571.951 kb on + strand, within PGA1_c15120 at 1571.961 kb on + strand, within PGA1_c15120 at 1571.963 kb on + strand, within PGA1_c15120 at 1571.971 kb on - strand, within PGA1_c15120 at 1572.129 kb on + strand, within PGA1_c15120 at 1572.184 kb on + strand, within PGA1_c15120 at 1572.192 kb on - strand, within PGA1_c15120 at 1572.393 kb on + strand, within PGA1_c15120 at 1572.393 kb on + strand, within PGA1_c15120 at 1572.393 kb on + strand, within PGA1_c15120 at 1572.487 kb on + strand at 1572.539 kb on + strand at 1572.539 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.592 kb on + strand at 1572.600 kb on - strand at 1572.600 kb on - strand at 1572.600 kb on - strand at 1572.638 kb on - strand at 1572.666 kb on - strand at 1572.693 kb on + strand at 1572.693 kb on + strand at 1572.696 kb on - strand at 1572.696 kb on - strand at 1572.701 kb on - strand at 1572.701 kb on - strand at 1572.706 kb on - strand at 1572.734 kb on - strand, within PGA1_c15130 at 1572.781 kb on + strand, within PGA1_c15130 at 1572.794 kb on - strand, within PGA1_c15130 at 1572.799 kb on - strand, within PGA1_c15130 at 1572.833 kb on + strand, within PGA1_c15130 at 1572.833 kb on + strand, within PGA1_c15130 at 1572.841 kb on - strand, within PGA1_c15130 at 1572.911 kb on - strand, within PGA1_c15130 at 1572.912 kb on - strand, within PGA1_c15130 at 1572.925 kb on - strand, within PGA1_c15130 at 1572.956 kb on - strand, within PGA1_c15130 at 1572.958 kb on + strand, within PGA1_c15130 at 1573.021 kb on - strand at 1573.031 kb on + strand at 1573.032 kb on + strand at 1573.045 kb on + strand at 1573.124 kb on + strand at 1573.165 kb on - strand at 1573.205 kb on + strand at 1573.205 kb on + strand at 1573.213 kb on - strand at 1573.213 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 1,571,089 + -1.5 1,571,168 - +0.2 1,571,179 + +0.4 1,571,343 + PGA1_c15120 0.15 -0.3 1,571,378 + PGA1_c15120 0.17 -0.1 1,571,398 - PGA1_c15120 0.18 -1.0 1,571,435 + PGA1_c15120 0.21 -1.1 1,571,549 - PGA1_c15120 0.29 -0.6 1,571,557 + PGA1_c15120 0.29 -1.5 1,571,593 + PGA1_c15120 0.32 -0.3 1,571,603 - PGA1_c15120 0.32 +0.5 1,571,625 + PGA1_c15120 0.34 +0.3 1,571,648 + PGA1_c15120 0.35 +0.6 1,571,648 + PGA1_c15120 0.35 -1.9 1,571,656 - PGA1_c15120 0.36 -2.5 1,571,736 - PGA1_c15120 0.41 +0.7 1,571,827 - PGA1_c15120 0.48 -2.8 1,571,951 + PGA1_c15120 0.56 +0.3 1,571,961 + PGA1_c15120 0.57 -0.8 1,571,963 + PGA1_c15120 0.57 +0.7 1,571,971 - PGA1_c15120 0.57 -0.5 1,572,129 + PGA1_c15120 0.68 -0.1 1,572,184 + PGA1_c15120 0.72 +0.9 1,572,192 - PGA1_c15120 0.72 -0.3 1,572,393 + PGA1_c15120 0.86 -1.5 1,572,393 + PGA1_c15120 0.86 -2.1 1,572,393 + PGA1_c15120 0.86 -3.6 1,572,487 + +0.7 1,572,539 + +1.1 1,572,539 + +1.0 1,572,592 + +0.1 1,572,592 + -0.4 1,572,592 + +1.4 1,572,592 + +1.4 1,572,592 + +0.3 1,572,592 + -1.9 1,572,592 + +0.2 1,572,592 + -0.3 1,572,592 + +0.4 1,572,592 + -0.2 1,572,592 + +0.9 1,572,592 + -0.7 1,572,592 + +0.9 1,572,592 + -0.0 1,572,592 + +0.6 1,572,592 + +0.7 1,572,592 + -0.7 1,572,592 + +0.4 1,572,592 + -0.7 1,572,592 + +2.2 1,572,600 - -0.3 1,572,600 - -0.9 1,572,600 - -0.5 1,572,638 - -0.6 1,572,666 - -4.3 1,572,693 + -0.0 1,572,693 + +1.2 1,572,696 - +0.5 1,572,696 - +0.4 1,572,701 - +0.9 1,572,701 - -1.0 1,572,706 - +0.7 1,572,734 - PGA1_c15130 0.11 +0.6 1,572,781 + PGA1_c15130 0.25 +0.7 1,572,794 - PGA1_c15130 0.28 -0.0 1,572,799 - PGA1_c15130 0.30 +0.1 1,572,833 + PGA1_c15130 0.39 +1.1 1,572,833 + PGA1_c15130 0.39 +0.9 1,572,841 - PGA1_c15130 0.42 +1.9 1,572,911 - PGA1_c15130 0.62 -0.9 1,572,912 - PGA1_c15130 0.62 -0.5 1,572,925 - PGA1_c15130 0.66 -0.1 1,572,956 - PGA1_c15130 0.74 +0.9 1,572,958 + PGA1_c15130 0.75 -0.9 1,573,021 - +0.5 1,573,031 + +0.1 1,573,032 + -0.1 1,573,045 + -1.0 1,573,124 + -0.7 1,573,165 - +1.1 1,573,205 + +1.8 1,573,205 + +0.2 1,573,213 - -0.4 1,573,213 - -0.4
Or see this region's nucleotide sequence