Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11470 and PGA1_c11480 overlap by 4 nucleotides PGA1_c11480 and PGA1_c11490 overlap by 4 nucleotides PGA1_c11490 and PGA1_c11500 are separated by 169 nucleotides
PGA1_c11470: PGA1_c11470 - acetyltransferase domain-containing protein, at 1,186,369 to 1,186,818
_c11470
PGA1_c11480: PGA1_c11480 - Uncharacterized conserved protein, at 1,186,815 to 1,187,342
_c11480
PGA1_c11490: PGA1_c11490 - pyrimidine-specific ribonucleoside hydrolase RihA, at 1,187,339 to 1,188,280
_c11490
PGA1_c11500: PGA1_c11500 - putative pyridoxal-phosphate-dependent aminotransferase, at 1,188,450 to 1,189,670
_c11500
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1186.450 kb on - strand, within PGA1_c11470 at 1186.507 kb on - strand, within PGA1_c11470 at 1186.512 kb on - strand, within PGA1_c11470 at 1186.617 kb on - strand, within PGA1_c11470 at 1186.719 kb on - strand, within PGA1_c11470 at 1186.772 kb on + strand, within PGA1_c11470 at 1186.782 kb on + strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.897 kb on - strand, within PGA1_c11480 at 1186.897 kb on - strand, within PGA1_c11480 at 1186.899 kb on - strand, within PGA1_c11480 at 1186.961 kb on + strand, within PGA1_c11480 at 1186.999 kb on - strand, within PGA1_c11480 at 1187.032 kb on + strand, within PGA1_c11480 at 1187.077 kb on + strand, within PGA1_c11480 at 1187.197 kb on - strand, within PGA1_c11480 at 1187.278 kb on - strand, within PGA1_c11480 at 1187.301 kb on - strand at 1187.303 kb on + strand at 1187.311 kb on - strand at 1187.311 kb on - strand at 1187.313 kb on + strand at 1187.337 kb on + strand at 1187.337 kb on + strand at 1187.397 kb on + strand at 1187.405 kb on - strand at 1187.405 kb on - strand at 1187.515 kb on + strand, within PGA1_c11490 at 1187.515 kb on + strand, within PGA1_c11490 at 1187.515 kb on + strand, within PGA1_c11490 at 1187.518 kb on - strand, within PGA1_c11490 at 1187.523 kb on - strand, within PGA1_c11490 at 1187.542 kb on + strand, within PGA1_c11490 at 1187.595 kb on - strand, within PGA1_c11490 at 1187.666 kb on - strand, within PGA1_c11490 at 1187.707 kb on + strand, within PGA1_c11490 at 1187.725 kb on + strand, within PGA1_c11490 at 1187.725 kb on + strand, within PGA1_c11490 at 1187.735 kb on - strand, within PGA1_c11490 at 1187.740 kb on - strand, within PGA1_c11490 at 1187.740 kb on - strand, within PGA1_c11490 at 1187.896 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.001 kb on + strand, within PGA1_c11490 at 1188.009 kb on - strand, within PGA1_c11490 at 1188.009 kb on - strand, within PGA1_c11490 at 1188.009 kb on - strand, within PGA1_c11490 at 1188.009 kb on - strand, within PGA1_c11490 at 1188.021 kb on + strand, within PGA1_c11490 at 1188.295 kb on - strand at 1188.332 kb on + strand at 1188.340 kb on - strand at 1188.374 kb on + strand at 1188.442 kb on - strand at 1188.442 kb on - strand at 1188.442 kb on - strand at 1188.587 kb on + strand, within PGA1_c11500 at 1188.638 kb on + strand, within PGA1_c11500 at 1188.654 kb on + strand, within PGA1_c11500 at 1188.654 kb on + strand, within PGA1_c11500 at 1188.766 kb on - strand, within PGA1_c11500 at 1188.879 kb on + strand, within PGA1_c11500 at 1188.887 kb on - strand, within PGA1_c11500 at 1188.889 kb on + strand, within PGA1_c11500 at 1189.079 kb on + strand, within PGA1_c11500 at 1189.243 kb on - strand, within PGA1_c11500 at 1189.251 kb on - strand, within PGA1_c11500
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 1,186,450 - PGA1_c11470 0.18 +0.3 1,186,507 - PGA1_c11470 0.31 +2.8 1,186,512 - PGA1_c11470 0.32 +0.6 1,186,617 - PGA1_c11470 0.55 -0.3 1,186,719 - PGA1_c11470 0.78 +0.5 1,186,772 + PGA1_c11470 0.90 +1.4 1,186,782 + -1.0 1,186,785 - +0.5 1,186,785 - +0.3 1,186,785 - -0.6 1,186,785 - +1.1 1,186,897 - PGA1_c11480 0.16 -0.2 1,186,897 - PGA1_c11480 0.16 +1.6 1,186,899 - PGA1_c11480 0.16 -0.4 1,186,961 + PGA1_c11480 0.28 -1.0 1,186,999 - PGA1_c11480 0.35 +2.0 1,187,032 + PGA1_c11480 0.41 +1.1 1,187,077 + PGA1_c11480 0.50 -0.6 1,187,197 - PGA1_c11480 0.72 +1.1 1,187,278 - PGA1_c11480 0.88 -1.3 1,187,301 - -0.2 1,187,303 + +1.3 1,187,311 - +1.0 1,187,311 - -1.4 1,187,313 + +3.2 1,187,337 + +0.4 1,187,337 + -0.8 1,187,397 + +0.2 1,187,405 - -1.4 1,187,405 - +1.7 1,187,515 + PGA1_c11490 0.19 +1.3 1,187,515 + PGA1_c11490 0.19 +0.7 1,187,515 + PGA1_c11490 0.19 -0.1 1,187,518 - PGA1_c11490 0.19 -2.0 1,187,523 - PGA1_c11490 0.20 +1.6 1,187,542 + PGA1_c11490 0.22 +1.2 1,187,595 - PGA1_c11490 0.27 -0.2 1,187,666 - PGA1_c11490 0.35 +0.4 1,187,707 + PGA1_c11490 0.39 +1.0 1,187,725 + PGA1_c11490 0.41 -0.0 1,187,725 + PGA1_c11490 0.41 +0.4 1,187,735 - PGA1_c11490 0.42 +0.9 1,187,740 - PGA1_c11490 0.43 -0.0 1,187,740 - PGA1_c11490 0.43 +0.2 1,187,896 + PGA1_c11490 0.59 +1.0 1,188,001 + PGA1_c11490 0.70 +0.4 1,188,001 + PGA1_c11490 0.70 +0.7 1,188,001 + PGA1_c11490 0.70 +1.3 1,188,001 + PGA1_c11490 0.70 +1.4 1,188,001 + PGA1_c11490 0.70 +0.7 1,188,001 + PGA1_c11490 0.70 +1.4 1,188,001 + PGA1_c11490 0.70 +0.6 1,188,009 - PGA1_c11490 0.71 -0.1 1,188,009 - PGA1_c11490 0.71 -0.0 1,188,009 - PGA1_c11490 0.71 -0.1 1,188,009 - PGA1_c11490 0.71 -1.4 1,188,021 + PGA1_c11490 0.72 +0.7 1,188,295 - -0.1 1,188,332 + -0.4 1,188,340 - +0.3 1,188,374 + +1.7 1,188,442 - +1.7 1,188,442 - +1.5 1,188,442 - +0.7 1,188,587 + PGA1_c11500 0.11 +0.2 1,188,638 + PGA1_c11500 0.15 -0.0 1,188,654 + PGA1_c11500 0.17 -1.8 1,188,654 + PGA1_c11500 0.17 +1.6 1,188,766 - PGA1_c11500 0.26 -0.3 1,188,879 + PGA1_c11500 0.35 -1.0 1,188,887 - PGA1_c11500 0.36 -1.6 1,188,889 + PGA1_c11500 0.36 -1.1 1,189,079 + PGA1_c11500 0.52 -0.6 1,189,243 - PGA1_c11500 0.65 -1.8 1,189,251 - PGA1_c11500 0.66 -2.0
Or see this region's nucleotide sequence