Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2476 and SO2477 are separated by 19 nucleotides SO2477 and kdsB are separated by 82 nucleotides kdsB and SO2479 are separated by 28 nucleotides
SO2476: SO2476 - polysaccharide biosynthesis protein (NCBI ptt file), at 2,596,996 to 2,598,183
SO2476
SO2477: SO2477 - alcohol dehydrogenase, iron-containing (NCBI ptt file), at 2,598,203 to 2,599,273
SO2477
SO2478: kdsB - 3-deoxy-D-manno-octulosonate cytidylyltransferase (NCBI ptt file), at 2,599,356 to 2,600,093
kdsB
SO2479: SO2479 - hypothetical protein (NCBI ptt file), at 2,600,122 to 2,600,358
SO2479
Position (kb)
2598
2599
2600 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2597.335 kb on + strand, within SO2476 at 2597.358 kb on - strand, within SO2476 at 2597.374 kb on + strand, within SO2476 at 2597.650 kb on + strand, within SO2476 at 2597.719 kb on + strand, within SO2476 at 2597.871 kb on - strand, within SO2476 at 2597.905 kb on - strand, within SO2476 at 2598.000 kb on + strand, within SO2476 at 2598.000 kb on + strand, within SO2476 at 2598.120 kb on + strand at 2598.120 kb on + strand at 2598.159 kb on + strand at 2598.282 kb on - strand at 2598.289 kb on + strand at 2598.289 kb on + strand at 2598.297 kb on - strand at 2598.297 kb on - strand at 2598.457 kb on - strand, within SO2477 at 2598.603 kb on + strand, within SO2477 at 2598.794 kb on - strand, within SO2477 at 2598.849 kb on - strand, within SO2477 at 2598.857 kb on + strand, within SO2477 at 2598.982 kb on + strand, within SO2477 at 2598.996 kb on + strand, within SO2477 at 2599.002 kb on - strand, within SO2477 at 2600.170 kb on + strand, within SO2479 at 2600.250 kb on + strand, within SO2479
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,597,335 + SO2476 0.29 -6.1 2,597,358 - SO2476 0.30 -3.0 2,597,374 + SO2476 0.32 -2.3 2,597,650 + SO2476 0.55 -4.0 2,597,719 + SO2476 0.61 -4.4 2,597,871 - SO2476 0.74 -2.8 2,597,905 - SO2476 0.77 -3.5 2,598,000 + SO2476 0.85 -3.3 2,598,000 + SO2476 0.85 -3.4 2,598,120 + -3.7 2,598,120 + -4.1 2,598,159 + -2.4 2,598,282 - -3.5 2,598,289 + -4.4 2,598,289 + -2.3 2,598,297 - -4.9 2,598,297 - -3.4 2,598,457 - SO2477 0.24 -5.5 2,598,603 + SO2477 0.37 -2.3 2,598,794 - SO2477 0.55 -2.7 2,598,849 - SO2477 0.60 -3.7 2,598,857 + SO2477 0.61 -5.8 2,598,982 + SO2477 0.73 -2.8 2,598,996 + SO2477 0.74 -2.5 2,599,002 - SO2477 0.75 -3.1 2,600,170 + SO2479 0.20 +0.3 2,600,250 + SO2479 0.54 -1.1
Or see this region's nucleotide sequence