Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapE and SO2472 are separated by 5 nucleotides SO2472 and SO2473 are separated by 69 nucleotides SO2473 and SO2474 are separated by 168 nucleotides
SO2471: dapE - succinyl-diaminopimelate desuccinylase (NCBI ptt file), at 2,593,058 to 2,594,212
dapE
SO2472: SO2472 - D-alanyl-D-alanine carboxypeptidase-related protein (NCBI ptt file), at 2,594,218 to 2,594,976
SO2472
SO2473: SO2473 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,595,046 to 2,595,879
SO2473
SO2474: SO2474 - carbonic anhydrase family protein (NCBI ptt file), at 2,596,048 to 2,596,653
SO2474
Position (kb)
2595
2596 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2594.244 kb on + strand at 2594.257 kb on + strand at 2594.257 kb on + strand at 2594.342 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.970 kb on + strand at 2595.096 kb on + strand at 2595.146 kb on + strand, within SO2473 at 2595.148 kb on + strand, within SO2473 at 2595.290 kb on + strand, within SO2473 at 2595.458 kb on + strand, within SO2473 at 2595.518 kb on - strand, within SO2473 at 2595.597 kb on - strand, within SO2473 at 2595.657 kb on + strand, within SO2473 at 2595.703 kb on + strand, within SO2473 at 2595.706 kb on - strand, within SO2473 at 2595.721 kb on - strand, within SO2473 at 2595.734 kb on - strand, within SO2473 at 2595.858 kb on - strand at 2595.908 kb on + strand at 2596.158 kb on + strand, within SO2474 at 2596.347 kb on - strand, within SO2474 at 2596.573 kb on + strand, within SO2474 at 2596.726 kb on - strand at 2596.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,594,244 + +0.4 2,594,257 + +1.2 2,594,257 + +1.4 2,594,342 + SO2472 0.16 +2.2 2,594,801 + SO2472 0.77 +1.3 2,594,801 + SO2472 0.77 +2.8 2,594,970 + -0.2 2,595,096 + +0.4 2,595,146 + SO2473 0.12 +0.7 2,595,148 + SO2473 0.12 +1.3 2,595,290 + SO2473 0.29 -0.3 2,595,458 + SO2473 0.49 -1.0 2,595,518 - SO2473 0.57 +0.3 2,595,597 - SO2473 0.66 -0.2 2,595,657 + SO2473 0.73 -0.3 2,595,703 + SO2473 0.79 -0.5 2,595,706 - SO2473 0.79 +1.0 2,595,721 - SO2473 0.81 -0.4 2,595,734 - SO2473 0.82 +0.0 2,595,858 - +0.2 2,595,908 + +1.1 2,596,158 + SO2474 0.18 +0.7 2,596,347 - SO2474 0.49 -0.0 2,596,573 + SO2474 0.87 -0.4 2,596,726 - -0.3 2,596,848 - +0.1
Or see this region's nucleotide sequence