Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1857 and tRNA-Ser-2 are separated by 221 nucleotides tRNA-Ser-2 and SO1858 are separated by 27 nucleotides SO1858 and tRNA-Ser-1 are separated by 357 nucleotides
SO1857: SO1857 - hypothetical ATP-dependent protease (NCBI ptt file), at 1,948,086 to 1,949,819
SO1857
SO_t031: tRNA-Ser-2 - tRNA-Ser (RefSeq), at 1,950,041 to 1,950,131
tRNA-Ser-2
SO1858: SO1858 - hypothetical protein (NCBI ptt file), at 1,950,159 to 1,950,755
SO1858
SO_t032: tRNA-Ser-1 - tRNA-Ser (RefSeq), at 1,951,113 to 1,951,203
tRNA-Ser-1
Position (kb)
1950
1951 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1949.278 kb on - strand, within SO1857 at 1949.328 kb on + strand, within SO1857 at 1949.364 kb on + strand, within SO1857 at 1949.444 kb on + strand, within SO1857 at 1949.514 kb on + strand, within SO1857 at 1949.744 kb on - strand at 1949.771 kb on + strand at 1949.832 kb on + strand at 1949.933 kb on - strand at 1949.980 kb on - strand at 1950.604 kb on - strand, within SO1858 at 1950.663 kb on + strand, within SO1858 at 1950.668 kb on + strand, within SO1858 at 1950.676 kb on - strand, within SO1858 at 1950.695 kb on + strand, within SO1858 at 1950.782 kb on - strand at 1950.784 kb on + strand at 1950.784 kb on + strand at 1950.792 kb on - strand at 1950.836 kb on + strand at 1950.923 kb on - strand at 1950.923 kb on - strand at 1950.925 kb on + strand at 1950.925 kb on + strand at 1950.933 kb on - strand at 1950.933 kb on - strand at 1950.954 kb on - strand at 1950.954 kb on - strand at 1950.962 kb on + strand at 1950.985 kb on - strand at 1950.986 kb on - strand at 1951.079 kb on + strand at 1951.580 kb on + strand at 1951.596 kb on + strand at 1951.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,949,278 - SO1857 0.69 -2.9 1,949,328 + SO1857 0.72 -3.3 1,949,364 + SO1857 0.74 -2.9 1,949,444 + SO1857 0.78 -1.8 1,949,514 + SO1857 0.82 -2.2 1,949,744 - -1.2 1,949,771 + -3.7 1,949,832 + -4.0 1,949,933 - +0.6 1,949,980 - -0.1 1,950,604 - SO1858 0.75 +0.0 1,950,663 + SO1858 0.84 +0.2 1,950,668 + SO1858 0.85 +0.1 1,950,676 - SO1858 0.87 +0.3 1,950,695 + SO1858 0.90 -0.6 1,950,782 - -0.2 1,950,784 + +0.1 1,950,784 + +0.2 1,950,792 - -0.1 1,950,836 + -0.2 1,950,923 - +0.3 1,950,923 - +0.4 1,950,925 + -0.6 1,950,925 + +0.5 1,950,933 - -0.3 1,950,933 - +0.3 1,950,954 - -0.2 1,950,954 - +0.3 1,950,962 + -0.1 1,950,985 - +0.8 1,950,986 - +0.6 1,951,079 + +0.4 1,951,580 + +0.5 1,951,596 + +0.0 1,951,719 + -0.0
Or see this region's nucleotide sequence