Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmA and murI are separated by 55 nucleotides murI and SO0208 are separated by 6 nucleotides SO0208 and Sp16SB are separated by 682 nucleotides
SO0206: trmA - tRNA (uracil-5-)-methyltransferase (NCBI ptt file), at 213,570 to 214,670
trmA
SO0207: murI - glutamate racemase (NCBI ptt file), at 214,726 to 215,634
murI
SO0208: SO0208 - RNA-binding protein (NCBI ptt file), at 215,641 to 216,105
SO0208
SO_r004: Sp16SB - 16S ribosomal RNA (RefSeq), at 216,788 to 218,316
Sp16SB
Position (kb) 215
216
217 Strain fitness (log2 ratio) -1
0
1
2 at 214.687 kb on + strand at 214.695 kb on - strand at 214.721 kb on + strand at 214.733 kb on + strand at 215.700 kb on + strand, within SO0208 at 215.708 kb on - strand, within SO0208 at 215.762 kb on + strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.782 kb on + strand, within SO0208 at 215.790 kb on - strand, within SO0208 at 215.839 kb on - strand, within SO0208 at 215.885 kb on - strand, within SO0208 at 215.893 kb on - strand, within SO0208 at 215.927 kb on + strand, within SO0208 at 215.950 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.041 kb on + strand, within SO0208 at 216.049 kb on - strand, within SO0208 at 216.243 kb on + strand at 216.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 214,687 + +0.9 214,695 - -0.4 214,721 + +0.3 214,733 + -0.1 215,700 + SO0208 0.13 +0.9 215,708 - SO0208 0.14 +1.2 215,762 + SO0208 0.26 +0.4 215,770 - SO0208 0.28 +1.0 215,770 - SO0208 0.28 +0.5 215,770 - SO0208 0.28 +0.8 215,782 + SO0208 0.30 +1.3 215,790 - SO0208 0.32 +0.9 215,839 - SO0208 0.43 +1.9 215,885 - SO0208 0.52 +0.8 215,893 - SO0208 0.54 +1.0 215,927 + SO0208 0.62 +0.9 215,950 + SO0208 0.66 +0.5 216,026 + SO0208 0.83 +0.9 216,026 + SO0208 0.83 +0.8 216,026 + SO0208 0.83 +2.4 216,026 + SO0208 0.83 +0.9 216,041 + SO0208 0.86 +1.2 216,049 - SO0208 0.88 +0.1 216,243 + +0.3 216,386 + +0.3
Or see this region's nucleotide sequence