Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspF and gspG are separated by 74 nucleotides gspG and gspH are separated by 1 nucleotides gspH and gspI overlap by 14 nucleotides gspI and gspJ overlap by 20 nucleotides
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
Position (kb) 177
178 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF at 177.010 kb on - strand, within gspF at 177.118 kb on + strand at 177.269 kb on + strand at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH at 178.221 kb on - strand, within gspH at 178.237 kb on + strand at 178.266 kb on - strand at 178.282 kb on + strand at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 176,289 - gspF SO0168 0.25 -4.4 176,327 + gspF SO0168 0.28 -2.9 176,327 + gspF SO0168 0.28 -3.8 176,335 - gspF SO0168 0.29 -2.8 176,382 - gspF SO0168 0.33 -2.4 176,382 - gspF SO0168 0.33 -2.9 176,524 - gspF SO0168 0.44 -4.2 176,555 - gspF SO0168 0.47 -2.2 176,597 - gspF SO0168 0.50 -2.4 176,640 - gspF SO0168 0.54 -0.8 176,795 - gspF SO0168 0.67 -2.6 176,834 + gspF SO0168 0.70 +0.1 176,834 + gspF SO0168 0.70 -2.3 176,936 + gspF SO0168 0.78 -2.3 176,941 + gspF SO0168 0.78 -2.6 176,941 + gspF SO0168 0.78 -3.4 176,941 + gspF SO0168 0.78 -0.8 176,953 + gspF SO0168 0.79 -3.3 176,958 + gspF SO0168 0.80 -2.1 176,958 + gspF SO0168 0.80 -3.5 176,966 - gspF SO0168 0.80 -3.9 176,966 - gspF SO0168 0.80 -3.8 177,010 - gspF SO0168 0.84 -2.9 177,118 + -2.4 177,269 + -0.2 177,277 - -3.5 177,303 + -3.6 177,303 + -2.5 177,329 + gspG SO0169 0.11 -4.8 177,364 - gspG SO0169 0.20 -3.2 177,366 - gspG SO0169 0.20 -2.6 177,397 - gspG SO0169 0.27 -3.4 177,476 + gspG SO0169 0.45 -2.9 177,492 + gspG SO0169 0.49 -3.0 177,554 + gspG SO0169 0.63 -2.7 177,613 - gspG SO0169 0.77 -1.5 177,638 + gspG SO0169 0.83 -4.1 177,662 + gspG SO0169 0.88 -3.7 177,751 - -3.0 177,788 + gspH SO0170 0.13 -3.0 177,837 + gspH SO0170 0.21 -3.4 177,837 + gspH SO0170 0.21 -1.1 177,837 + gspH SO0170 0.21 -1.7 177,845 - gspH SO0170 0.23 -2.5 177,845 - gspH SO0170 0.23 -3.5 178,065 - gspH SO0170 0.61 -2.7 178,221 - gspH SO0170 0.88 -3.1 178,237 + -2.5 178,266 - -2.5 178,282 + -0.4 178,440 + gspI SO0171 0.45 -1.6 178,516 - gspI SO0171 0.66 -3.0 178,605 - gspI SO0171 0.90 -1.6 178,654 + -0.5 178,654 + -2.8 178,654 + -4.8 178,654 + -2.7 178,654 + -3.4 178,654 + -2.5 178,662 - -3.4 178,662 - -3.7 178,700 + gspJ SO0172 0.10 -2.9
Or see this region's nucleotide sequence