Experiment: m.b. Gentamicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03100 and PGA1_c03110 are separated by 33 nucleotides PGA1_c03110 and PGA1_c03120 are separated by 32 nucleotides PGA1_c03120 and PGA1_c03130 are separated by 178 nucleotides PGA1_c03130 and PGA1_c03140 are separated by 35 nucleotides
PGA1_c03100: PGA1_c03100 - putative aminoglycoside efflux pump, at 296,920 to 300,315
_c03100
PGA1_c03110: PGA1_c03110 - NUDIX hydrolase, at 300,349 to 300,858
_c03110
PGA1_c03120: PGA1_c03120 - hypothetical protein, at 300,891 to 301,745
_c03120
PGA1_c03130: PGA1_c03130 - Putative transcriptional regulator, at 301,924 to 302,481
_c03130
PGA1_c03140: PGA1_c03140 - putative tRNA threonylcarbamoyladenosine biosynthesis protein, at 302,517 to 303,464
_c03140
Position (kb) 300
301
302 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 299.895 kb on + strand, within PGA1_c03100 at 299.949 kb on + strand, within PGA1_c03100 at 299.957 kb on - strand, within PGA1_c03100 at 300.019 kb on + strand at 300.019 kb on + strand at 300.089 kb on + strand at 300.205 kb on + strand at 300.215 kb on + strand at 300.319 kb on + strand at 300.425 kb on - strand, within PGA1_c03110 at 300.430 kb on - strand, within PGA1_c03110 at 300.539 kb on + strand, within PGA1_c03110 at 300.612 kb on + strand, within PGA1_c03110 at 300.620 kb on - strand, within PGA1_c03110 at 300.669 kb on - strand, within PGA1_c03110 at 300.707 kb on - strand, within PGA1_c03110 at 300.713 kb on + strand, within PGA1_c03110 at 300.743 kb on - strand, within PGA1_c03110 at 300.859 kb on + strand at 300.864 kb on + strand at 300.906 kb on + strand at 300.906 kb on + strand at 300.976 kb on + strand at 300.976 kb on + strand at 300.984 kb on - strand, within PGA1_c03120 at 301.036 kb on + strand, within PGA1_c03120 at 301.110 kb on - strand, within PGA1_c03120 at 301.172 kb on - strand, within PGA1_c03120 at 301.274 kb on + strand, within PGA1_c03120 at 301.282 kb on + strand, within PGA1_c03120 at 301.282 kb on - strand, within PGA1_c03120 at 301.302 kb on - strand, within PGA1_c03120 at 301.390 kb on - strand, within PGA1_c03120 at 301.435 kb on + strand, within PGA1_c03120 at 301.565 kb on + strand, within PGA1_c03120 at 301.687 kb on + strand at 301.687 kb on + strand at 301.713 kb on + strand at 301.733 kb on + strand at 301.741 kb on - strand at 301.750 kb on + strand at 301.753 kb on - strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.830 kb on + strand at 301.832 kb on - strand at 301.837 kb on - strand at 301.865 kb on + strand at 301.884 kb on + strand at 301.907 kb on + strand at 301.907 kb on + strand at 301.927 kb on + strand at 301.969 kb on - strand at 302.008 kb on + strand, within PGA1_c03130 at 302.008 kb on + strand, within PGA1_c03130 at 302.008 kb on + strand, within PGA1_c03130 at 302.016 kb on - strand, within PGA1_c03130 at 302.016 kb on - strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.119 kb on + strand, within PGA1_c03130 at 302.153 kb on - strand, within PGA1_c03130 at 302.157 kb on + strand, within PGA1_c03130 at 302.165 kb on - strand, within PGA1_c03130 at 302.170 kb on - strand, within PGA1_c03130 at 302.170 kb on - strand, within PGA1_c03130 at 302.201 kb on + strand, within PGA1_c03130 at 302.223 kb on - strand, within PGA1_c03130 at 302.233 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.294 kb on - strand, within PGA1_c03130 at 302.294 kb on - strand, within PGA1_c03130 at 302.301 kb on + strand, within PGA1_c03130 at 302.301 kb on + strand, within PGA1_c03130 at 302.312 kb on + strand, within PGA1_c03130 at 302.375 kb on + strand, within PGA1_c03130 at 302.375 kb on + strand, within PGA1_c03130 at 302.438 kb on + strand at 302.454 kb on - strand at 302.463 kb on - strand at 302.465 kb on + strand at 302.470 kb on + strand at 302.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.002 mg/ml remove 299,895 + PGA1_c03100 0.88 +1.3 299,949 + PGA1_c03100 0.89 +0.9 299,957 - PGA1_c03100 0.89 -1.4 300,019 + +0.9 300,019 + +0.7 300,089 + -0.4 300,205 + +1.5 300,215 + +1.3 300,319 + +2.5 300,425 - PGA1_c03110 0.15 +0.9 300,430 - PGA1_c03110 0.16 +1.3 300,539 + PGA1_c03110 0.37 +0.5 300,612 + PGA1_c03110 0.52 +0.3 300,620 - PGA1_c03110 0.53 +0.9 300,669 - PGA1_c03110 0.63 -0.1 300,707 - PGA1_c03110 0.70 +0.6 300,713 + PGA1_c03110 0.71 +1.3 300,743 - PGA1_c03110 0.77 +0.9 300,859 + -0.4 300,864 + +0.5 300,906 + -0.1 300,906 + +1.2 300,976 + -1.1 300,976 + -1.7 300,984 - PGA1_c03120 0.11 -0.9 301,036 + PGA1_c03120 0.17 +0.9 301,110 - PGA1_c03120 0.26 -0.1 301,172 - PGA1_c03120 0.33 -2.0 301,274 + PGA1_c03120 0.45 -1.3 301,282 + PGA1_c03120 0.46 -2.1 301,282 - PGA1_c03120 0.46 -0.1 301,302 - PGA1_c03120 0.48 -1.1 301,390 - PGA1_c03120 0.58 -0.7 301,435 + PGA1_c03120 0.64 -0.7 301,565 + PGA1_c03120 0.79 -2.6 301,687 + -1.1 301,687 + -2.4 301,713 + -0.3 301,733 + -0.4 301,741 - -1.7 301,750 + -1.5 301,753 - +0.1 301,829 + -1.9 301,829 + +1.1 301,829 + +1.5 301,829 + -1.4 301,830 + -0.2 301,832 - -0.7 301,837 - +1.9 301,865 + +0.5 301,884 + +0.5 301,907 + +0.2 301,907 + -0.8 301,927 + +0.7 301,969 - +0.5 302,008 + PGA1_c03130 0.15 -1.3 302,008 + PGA1_c03130 0.15 +0.7 302,008 + PGA1_c03130 0.15 -1.7 302,016 - PGA1_c03130 0.16 -0.1 302,016 - PGA1_c03130 0.16 -1.7 302,105 + PGA1_c03130 0.32 -1.4 302,105 + PGA1_c03130 0.32 -0.5 302,105 + PGA1_c03130 0.32 -0.1 302,105 + PGA1_c03130 0.32 -0.5 302,105 + PGA1_c03130 0.32 +0.5 302,105 + PGA1_c03130 0.32 +0.7 302,105 + PGA1_c03130 0.32 +0.6 302,105 + PGA1_c03130 0.32 +0.5 302,105 + PGA1_c03130 0.32 -1.9 302,105 + PGA1_c03130 0.32 -0.8 302,105 + PGA1_c03130 0.32 -0.7 302,105 + PGA1_c03130 0.32 +0.9 302,105 + PGA1_c03130 0.32 +2.9 302,105 + PGA1_c03130 0.32 +1.9 302,105 + PGA1_c03130 0.32 -0.1 302,105 + PGA1_c03130 0.32 +0.2 302,105 + PGA1_c03130 0.32 +0.8 302,105 + PGA1_c03130 0.32 +0.9 302,105 + PGA1_c03130 0.32 -0.1 302,113 - PGA1_c03130 0.34 +0.1 302,113 - PGA1_c03130 0.34 -1.1 302,113 - PGA1_c03130 0.34 -0.7 302,113 - PGA1_c03130 0.34 +1.9 302,113 - PGA1_c03130 0.34 +0.9 302,113 - PGA1_c03130 0.34 -0.5 302,113 - PGA1_c03130 0.34 +1.9 302,113 - PGA1_c03130 0.34 +0.9 302,113 - PGA1_c03130 0.34 +1.2 302,113 - PGA1_c03130 0.34 +1.7 302,119 + PGA1_c03130 0.35 +1.9 302,153 - PGA1_c03130 0.41 -0.4 302,157 + PGA1_c03130 0.42 +0.2 302,165 - PGA1_c03130 0.43 +1.1 302,170 - PGA1_c03130 0.44 -2.1 302,170 - PGA1_c03130 0.44 -0.6 302,201 + PGA1_c03130 0.50 -0.7 302,223 - PGA1_c03130 0.54 -0.1 302,233 + PGA1_c03130 0.55 -1.1 302,276 + PGA1_c03130 0.63 -0.3 302,276 + PGA1_c03130 0.63 +0.9 302,276 + PGA1_c03130 0.63 -0.7 302,276 + PGA1_c03130 0.63 +0.5 302,276 + PGA1_c03130 0.63 +2.7 302,276 + PGA1_c03130 0.63 -1.9 302,276 + PGA1_c03130 0.63 -0.7 302,276 + PGA1_c03130 0.63 +2.7 302,276 + PGA1_c03130 0.63 +0.2 302,276 + PGA1_c03130 0.63 -1.9 302,276 + PGA1_c03130 0.63 -0.2 302,284 - PGA1_c03130 0.65 -0.3 302,284 - PGA1_c03130 0.65 +0.7 302,284 - PGA1_c03130 0.65 +0.6 302,284 - PGA1_c03130 0.65 -0.7 302,284 - PGA1_c03130 0.65 -1.9 302,284 - PGA1_c03130 0.65 -1.3 302,284 - PGA1_c03130 0.65 +1.9 302,284 - PGA1_c03130 0.65 +2.1 302,284 - PGA1_c03130 0.65 -0.6 302,284 - PGA1_c03130 0.65 +1.5 302,284 - PGA1_c03130 0.65 +0.5 302,286 + PGA1_c03130 0.65 +1.6 302,286 + PGA1_c03130 0.65 +1.2 302,286 + PGA1_c03130 0.65 +0.2 302,286 + PGA1_c03130 0.65 +1.9 302,294 - PGA1_c03130 0.66 -1.9 302,294 - PGA1_c03130 0.66 -0.1 302,301 + PGA1_c03130 0.68 -1.0 302,301 + PGA1_c03130 0.68 -0.8 302,312 + PGA1_c03130 0.70 -0.7 302,375 + PGA1_c03130 0.81 -1.0 302,375 + PGA1_c03130 0.81 -0.1 302,438 + -0.5 302,454 - -1.1 302,463 - -0.4 302,465 + +3.7 302,470 + +3.2 302,503 - +0.1
Or see this region's nucleotide sequence