Experiment: m.b. Gentamicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02100 and PGA1_c02110 are separated by 66 nucleotides PGA1_c02110 and PGA1_c02120 are separated by 164 nucleotides PGA1_c02120 and PGA1_c02130 are separated by 5 nucleotides
PGA1_c02100: PGA1_c02100 - diguanylate cyclase (GGDEF) domain-containing protein, at 192,908 to 194,452
_c02100
PGA1_c02110: PGA1_c02110 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 194,519 to 195,406
_c02110
PGA1_c02120: PGA1_c02120 - hypothetical protein, at 195,571 to 196,143
_c02120
PGA1_c02130: PGA1_c02130 - conserved hypothetical protein YidD, at 196,149 to 196,421
_c02130
Position (kb) 194
195
196 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 193.524 kb on + strand, within PGA1_c02100 at 193.591 kb on + strand, within PGA1_c02100 at 193.599 kb on - strand, within PGA1_c02100 at 193.649 kb on - strand, within PGA1_c02100 at 193.795 kb on + strand, within PGA1_c02100 at 193.800 kb on + strand, within PGA1_c02100 at 193.834 kb on + strand, within PGA1_c02100 at 193.834 kb on + strand, within PGA1_c02100 at 193.903 kb on - strand, within PGA1_c02100 at 193.930 kb on - strand, within PGA1_c02100 at 193.985 kb on - strand, within PGA1_c02100 at 194.019 kb on + strand, within PGA1_c02100 at 194.027 kb on - strand, within PGA1_c02100 at 194.072 kb on - strand, within PGA1_c02100 at 194.151 kb on - strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.174 kb on + strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.182 kb on - strand, within PGA1_c02100 at 194.204 kb on - strand, within PGA1_c02100 at 194.240 kb on + strand, within PGA1_c02100 at 194.248 kb on - strand, within PGA1_c02100 at 194.248 kb on - strand, within PGA1_c02100 at 194.430 kb on - strand at 194.453 kb on + strand at 194.453 kb on + strand at 194.461 kb on - strand at 194.468 kb on - strand at 194.494 kb on - strand at 194.500 kb on - strand at 194.596 kb on + strand at 194.604 kb on - strand at 194.625 kb on + strand, within PGA1_c02110 at 194.633 kb on - strand, within PGA1_c02110 at 194.659 kb on + strand, within PGA1_c02110 at 194.669 kb on + strand, within PGA1_c02110 at 194.719 kb on + strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.946 kb on - strand, within PGA1_c02110 at 194.975 kb on - strand, within PGA1_c02110 at 194.991 kb on + strand, within PGA1_c02110 at 194.992 kb on - strand, within PGA1_c02110 at 195.025 kb on - strand, within PGA1_c02110 at 195.128 kb on - strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.208 kb on + strand, within PGA1_c02110 at 195.210 kb on + strand, within PGA1_c02110 at 195.210 kb on + strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.216 kb on - strand, within PGA1_c02110 at 195.218 kb on + strand, within PGA1_c02110 at 195.255 kb on - strand, within PGA1_c02110 at 195.272 kb on + strand, within PGA1_c02110 at 195.415 kb on - strand at 195.475 kb on - strand at 195.486 kb on - strand at 195.530 kb on + strand at 195.556 kb on + strand at 195.556 kb on + strand at 195.562 kb on + strand at 195.562 kb on + strand at 195.564 kb on - strand at 195.570 kb on - strand at 195.617 kb on + strand at 195.672 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.768 kb on + strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.776 kb on - strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.778 kb on + strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.786 kb on - strand, within PGA1_c02120 at 195.788 kb on + strand, within PGA1_c02120 at 195.796 kb on - strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.821 kb on + strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.829 kb on - strand, within PGA1_c02120 at 195.857 kb on - strand, within PGA1_c02120 at 195.866 kb on + strand, within PGA1_c02120 at 195.891 kb on + strand, within PGA1_c02120 at 195.942 kb on - strand, within PGA1_c02120 at 195.971 kb on - strand, within PGA1_c02120 at 195.979 kb on - strand, within PGA1_c02120 at 196.060 kb on + strand, within PGA1_c02120 at 196.084 kb on + strand, within PGA1_c02120 at 196.100 kb on + strand at 196.108 kb on - strand at 196.118 kb on - strand at 196.127 kb on + strand at 196.147 kb on + strand at 196.198 kb on - strand, within PGA1_c02130 at 196.211 kb on - strand, within PGA1_c02130 at 196.360 kb on + strand, within PGA1_c02130 at 196.373 kb on + strand, within PGA1_c02130
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.002 mg/ml remove 193,524 + PGA1_c02100 0.40 -0.5 193,591 + PGA1_c02100 0.44 -1.8 193,599 - PGA1_c02100 0.45 -1.1 193,649 - PGA1_c02100 0.48 -0.1 193,795 + PGA1_c02100 0.57 +3.5 193,800 + PGA1_c02100 0.58 -1.7 193,834 + PGA1_c02100 0.60 -1.3 193,834 + PGA1_c02100 0.60 -3.9 193,903 - PGA1_c02100 0.64 -2.9 193,930 - PGA1_c02100 0.66 +0.8 193,985 - PGA1_c02100 0.70 +0.5 194,019 + PGA1_c02100 0.72 -0.3 194,027 - PGA1_c02100 0.72 +0.9 194,072 - PGA1_c02100 0.75 -0.4 194,151 - PGA1_c02100 0.80 -1.5 194,174 + PGA1_c02100 0.82 -1.1 194,174 + PGA1_c02100 0.82 -0.9 194,174 + PGA1_c02100 0.82 -1.1 194,174 + PGA1_c02100 0.82 -0.4 194,182 - PGA1_c02100 0.82 -1.3 194,182 - PGA1_c02100 0.82 -1.9 194,182 - PGA1_c02100 0.82 -0.6 194,182 - PGA1_c02100 0.82 -0.1 194,182 - PGA1_c02100 0.82 -0.4 194,204 - PGA1_c02100 0.84 +0.8 194,240 + PGA1_c02100 0.86 -1.1 194,248 - PGA1_c02100 0.87 +0.9 194,248 - PGA1_c02100 0.87 +0.2 194,430 - -0.8 194,453 + -1.6 194,453 + -0.5 194,461 - +0.9 194,468 - -0.1 194,494 - +0.1 194,500 - -2.0 194,596 + +1.7 194,604 - +0.3 194,625 + PGA1_c02110 0.12 +0.9 194,633 - PGA1_c02110 0.13 -0.1 194,659 + PGA1_c02110 0.16 +0.8 194,669 + PGA1_c02110 0.17 -1.1 194,719 + PGA1_c02110 0.23 +0.8 194,946 - PGA1_c02110 0.48 +0.4 194,946 - PGA1_c02110 0.48 +0.1 194,946 - PGA1_c02110 0.48 +0.9 194,946 - PGA1_c02110 0.48 -0.9 194,975 - PGA1_c02110 0.51 +0.6 194,991 + PGA1_c02110 0.53 +0.2 194,992 - PGA1_c02110 0.53 -0.3 195,025 - PGA1_c02110 0.57 +0.8 195,128 - PGA1_c02110 0.69 +0.8 195,208 + PGA1_c02110 0.78 +1.2 195,208 + PGA1_c02110 0.78 -1.1 195,208 + PGA1_c02110 0.78 -0.4 195,208 + PGA1_c02110 0.78 -0.5 195,208 + PGA1_c02110 0.78 +1.1 195,208 + PGA1_c02110 0.78 +0.2 195,208 + PGA1_c02110 0.78 -0.8 195,208 + PGA1_c02110 0.78 -1.1 195,208 + PGA1_c02110 0.78 -2.2 195,208 + PGA1_c02110 0.78 +0.9 195,208 + PGA1_c02110 0.78 +0.4 195,208 + PGA1_c02110 0.78 +1.9 195,208 + PGA1_c02110 0.78 +0.2 195,208 + PGA1_c02110 0.78 +0.5 195,208 + PGA1_c02110 0.78 +0.9 195,208 + PGA1_c02110 0.78 +1.3 195,208 + PGA1_c02110 0.78 +0.5 195,208 + PGA1_c02110 0.78 +1.1 195,208 + PGA1_c02110 0.78 +0.3 195,208 + PGA1_c02110 0.78 +0.9 195,208 + PGA1_c02110 0.78 +0.1 195,210 + PGA1_c02110 0.78 +0.6 195,210 + PGA1_c02110 0.78 +0.3 195,216 - PGA1_c02110 0.78 +0.5 195,216 - PGA1_c02110 0.78 -0.1 195,216 - PGA1_c02110 0.78 -2.1 195,216 - PGA1_c02110 0.78 +0.9 195,216 - PGA1_c02110 0.78 -0.1 195,216 - PGA1_c02110 0.78 +0.4 195,216 - PGA1_c02110 0.78 +2.4 195,216 - PGA1_c02110 0.78 +0.3 195,216 - PGA1_c02110 0.78 -0.1 195,216 - PGA1_c02110 0.78 -0.5 195,216 - PGA1_c02110 0.78 +0.5 195,216 - PGA1_c02110 0.78 +0.4 195,216 - PGA1_c02110 0.78 -0.4 195,216 - PGA1_c02110 0.78 +0.7 195,216 - PGA1_c02110 0.78 -0.9 195,216 - PGA1_c02110 0.78 -0.4 195,216 - PGA1_c02110 0.78 +0.5 195,216 - PGA1_c02110 0.78 -1.9 195,216 - PGA1_c02110 0.78 +2.4 195,216 - PGA1_c02110 0.78 -1.4 195,216 - PGA1_c02110 0.78 -0.5 195,216 - PGA1_c02110 0.78 +0.9 195,216 - PGA1_c02110 0.78 -0.6 195,216 - PGA1_c02110 0.78 -0.1 195,216 - PGA1_c02110 0.78 +0.4 195,216 - PGA1_c02110 0.78 +3.5 195,216 - PGA1_c02110 0.78 -1.1 195,216 - PGA1_c02110 0.78 +0.9 195,216 - PGA1_c02110 0.78 +0.9 195,216 - PGA1_c02110 0.78 -0.4 195,218 + PGA1_c02110 0.79 +0.9 195,255 - PGA1_c02110 0.83 +0.5 195,272 + PGA1_c02110 0.85 +0.1 195,415 - +0.8 195,475 - +0.9 195,486 - +0.9 195,530 + -1.1 195,556 + +0.5 195,556 + +0.3 195,562 + +0.4 195,562 + -1.1 195,564 - -0.3 195,570 - -1.4 195,617 + -0.1 195,672 + PGA1_c02120 0.18 -0.6 195,768 + PGA1_c02120 0.34 -1.9 195,768 + PGA1_c02120 0.34 -0.1 195,776 - PGA1_c02120 0.36 -0.1 195,776 - PGA1_c02120 0.36 -1.6 195,776 - PGA1_c02120 0.36 -0.4 195,778 + PGA1_c02120 0.36 +3.6 195,778 + PGA1_c02120 0.36 +2.4 195,786 - PGA1_c02120 0.38 +0.2 195,786 - PGA1_c02120 0.38 -0.1 195,788 + PGA1_c02120 0.38 +0.3 195,796 - PGA1_c02120 0.39 -1.4 195,821 + PGA1_c02120 0.44 +0.7 195,821 + PGA1_c02120 0.44 +1.1 195,821 + PGA1_c02120 0.44 -0.1 195,829 - PGA1_c02120 0.45 -0.5 195,829 - PGA1_c02120 0.45 -2.5 195,829 - PGA1_c02120 0.45 -0.9 195,857 - PGA1_c02120 0.50 +1.9 195,866 + PGA1_c02120 0.51 -2.6 195,891 + PGA1_c02120 0.56 -0.5 195,942 - PGA1_c02120 0.65 -2.4 195,971 - PGA1_c02120 0.70 +0.5 195,979 - PGA1_c02120 0.71 +1.9 196,060 + PGA1_c02120 0.85 -0.6 196,084 + PGA1_c02120 0.90 -0.6 196,100 + +0.7 196,108 - -0.6 196,118 - +0.9 196,127 + -1.4 196,147 + +0.9 196,198 - PGA1_c02130 0.18 +0.9 196,211 - PGA1_c02130 0.23 +0.9 196,360 + PGA1_c02130 0.77 -0.3 196,373 + PGA1_c02130 0.82 -0.3
Or see this region's nucleotide sequence