Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3278 and Dsui_3279 are separated by 13 nucleotides Dsui_3279 and Dsui_3280 are separated by 89 nucleotides Dsui_3280 and Dsui_3281 overlap by 4 nucleotides
Dsui_3278: Dsui_3278 - molybdenum cofactor biosynthesis protein A, at 3,515,354 to 3,516,385
_3278
Dsui_3279: Dsui_3279 - formate dehydrogenase family accessory protein FdhD, at 3,516,399 to 3,517,352
_3279
Dsui_3280: Dsui_3280 - formate dehydrogenase, gamma subunit, at 3,517,442 to 3,518,062
_3280
Dsui_3281: Dsui_3281 - formate dehydrogenase, beta subunit, Fe-S containing, at 3,518,059 to 3,518,949
_3281
Position (kb)
3516
3517
3518 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3515.414 kb on + strand at 3515.414 kb on + strand at 3515.420 kb on + strand at 3515.420 kb on + strand at 3515.422 kb on - strand at 3515.427 kb on + strand at 3515.428 kb on - strand at 3515.475 kb on + strand, within Dsui_3278 at 3515.483 kb on - strand, within Dsui_3278 at 3515.506 kb on - strand, within Dsui_3278 at 3515.536 kb on + strand at 3515.566 kb on - strand, within Dsui_3278 at 3515.577 kb on - strand, within Dsui_3278 at 3515.578 kb on - strand, within Dsui_3278 at 3515.669 kb on - strand, within Dsui_3278 at 3515.747 kb on + strand at 3515.799 kb on - strand, within Dsui_3278 at 3515.811 kb on + strand, within Dsui_3278 at 3515.819 kb on - strand, within Dsui_3278 at 3515.851 kb on - strand, within Dsui_3278 at 3515.865 kb on + strand, within Dsui_3278 at 3515.865 kb on - strand, within Dsui_3278 at 3515.905 kb on + strand at 3516.027 kb on - strand, within Dsui_3278 at 3516.027 kb on - strand, within Dsui_3278 at 3516.027 kb on - strand, within Dsui_3278 at 3516.079 kb on - strand, within Dsui_3278 at 3516.152 kb on - strand, within Dsui_3278 at 3516.191 kb on + strand at 3516.191 kb on + strand at 3516.293 kb on - strand at 3516.295 kb on + strand at 3516.453 kb on + strand at 3516.461 kb on - strand at 3516.472 kb on - strand at 3516.516 kb on + strand at 3516.538 kb on - strand, within Dsui_3279 at 3516.656 kb on + strand at 3516.671 kb on + strand at 3516.679 kb on - strand, within Dsui_3279 at 3516.773 kb on + strand at 3516.781 kb on - strand, within Dsui_3279 at 3517.064 kb on - strand, within Dsui_3279 at 3517.075 kb on - strand, within Dsui_3279 at 3517.090 kb on - strand, within Dsui_3279 at 3517.122 kb on + strand at 3517.130 kb on - strand, within Dsui_3279 at 3517.130 kb on - strand, within Dsui_3279 at 3517.206 kb on + strand at 3517.238 kb on - strand, within Dsui_3279 at 3517.267 kb on - strand at 3517.286 kb on + strand at 3517.361 kb on + strand at 3517.361 kb on + strand at 3517.369 kb on + strand at 3517.369 kb on - strand at 3517.507 kb on - strand, within Dsui_3280 at 3517.552 kb on + strand at 3517.557 kb on + strand at 3517.628 kb on + strand at 3517.628 kb on + strand at 3517.636 kb on - strand, within Dsui_3280 at 3517.691 kb on + strand, within Dsui_3280 at 3517.725 kb on - strand, within Dsui_3280 at 3517.729 kb on - strand, within Dsui_3280 at 3517.786 kb on + strand at 3517.910 kb on - strand, within Dsui_3280 at 3517.951 kb on - strand, within Dsui_3280 at 3517.951 kb on - strand, within Dsui_3280 at 3517.987 kb on + strand, within Dsui_3280 at 3517.987 kb on - strand, within Dsui_3280 at 3518.079 kb on - strand at 3518.107 kb on - strand at 3518.137 kb on - strand at 3518.137 kb on - strand at 3518.154 kb on + strand, within Dsui_3281 at 3518.171 kb on + strand at 3518.179 kb on - strand, within Dsui_3281 at 3518.179 kb on - strand, within Dsui_3281 at 3518.181 kb on + strand, within Dsui_3281 at 3518.183 kb on + strand at 3518.207 kb on - strand, within Dsui_3281 at 3518.249 kb on - strand, within Dsui_3281 at 3518.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 3,515,414 + -0.2 3,515,414 + +1.4 3,515,420 + -0.6 3,515,420 + -0.5 3,515,422 - -1.1 3,515,427 + +2.4 3,515,428 - -0.2 3,515,475 + Dsui_3278 0.12 +0.8 3,515,483 - Dsui_3278 0.12 -0.4 3,515,506 - Dsui_3278 0.15 -0.3 3,515,536 + +0.8 3,515,566 - Dsui_3278 0.21 +0.3 3,515,577 - Dsui_3278 0.22 -0.3 3,515,578 - Dsui_3278 0.22 -0.2 3,515,669 - Dsui_3278 0.31 -0.4 3,515,747 + +1.8 3,515,799 - Dsui_3278 0.43 +1.5 3,515,811 + Dsui_3278 0.44 +1.4 3,515,819 - Dsui_3278 0.45 +1.0 3,515,851 - Dsui_3278 0.48 +0.5 3,515,865 + Dsui_3278 0.50 -0.7 3,515,865 - Dsui_3278 0.50 -0.7 3,515,905 + +2.4 3,516,027 - Dsui_3278 0.65 -0.3 3,516,027 - Dsui_3278 0.65 -0.8 3,516,027 - Dsui_3278 0.65 +0.3 3,516,079 - Dsui_3278 0.70 +0.2 3,516,152 - Dsui_3278 0.77 -0.2 3,516,191 + +1.0 3,516,191 + +0.8 3,516,293 - -0.8 3,516,295 + +1.4 3,516,453 + +2.4 3,516,461 - +0.1 3,516,472 - +1.1 3,516,516 + +1.8 3,516,538 - Dsui_3279 0.15 -0.3 3,516,656 + +1.4 3,516,671 + +1.8 3,516,679 - Dsui_3279 0.29 -0.7 3,516,773 + +2.4 3,516,781 - Dsui_3279 0.40 -0.5 3,517,064 - Dsui_3279 0.70 -0.6 3,517,075 - Dsui_3279 0.71 +0.2 3,517,090 - Dsui_3279 0.72 -0.2 3,517,122 + -0.6 3,517,130 - Dsui_3279 0.77 -1.5 3,517,130 - Dsui_3279 0.77 -0.4 3,517,206 + -0.5 3,517,238 - Dsui_3279 0.88 -0.1 3,517,267 - +1.4 3,517,286 + +0.5 3,517,361 + -0.2 3,517,361 + +2.4 3,517,369 + +0.0 3,517,369 - +0.3 3,517,507 - Dsui_3280 0.10 +1.0 3,517,552 + +1.8 3,517,557 + +1.8 3,517,628 + +3.4 3,517,628 + -0.5 3,517,636 - Dsui_3280 0.31 +0.0 3,517,691 + Dsui_3280 0.40 -1.1 3,517,725 - Dsui_3280 0.46 +0.4 3,517,729 - Dsui_3280 0.46 -1.0 3,517,786 + +1.4 3,517,910 - Dsui_3280 0.75 +0.1 3,517,951 - Dsui_3280 0.82 +0.0 3,517,951 - Dsui_3280 0.82 +0.0 3,517,987 + Dsui_3280 0.88 -0.6 3,517,987 - Dsui_3280 0.88 -0.1 3,518,079 - -0.5 3,518,107 - +0.2 3,518,137 - -1.2 3,518,137 - -0.6 3,518,154 + Dsui_3281 0.11 +0.0 3,518,171 + +1.4 3,518,179 - Dsui_3281 0.13 -0.1 3,518,179 - Dsui_3281 0.13 -0.6 3,518,181 + Dsui_3281 0.14 +1.4 3,518,183 + +0.8 3,518,207 - Dsui_3281 0.17 +0.7 3,518,249 - Dsui_3281 0.21 -0.5 3,518,292 + +1.4
Or see this region's nucleotide sequence