Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3096 and Dsui_3097 are separated by 196 nucleotides Dsui_3097 and Dsui_3098 overlap by 4 nucleotides Dsui_3098 and Dsui_3099 overlap by 4 nucleotides
Dsui_3096: Dsui_3096 - NADH dehydrogenase, FAD-containing subunit, at 3,313,813 to 3,315,132
_3096
Dsui_3097: Dsui_3097 - O-6-methylguanine DNA methyltransferase, at 3,315,329 to 3,316,552
_3097
Dsui_3098: Dsui_3098 - alkylated DNA repair protein, at 3,316,549 to 3,317,211
_3098
Dsui_3099: Dsui_3099 - 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase, at 3,317,208 to 3,318,188
_3099
Position (kb)
3315
3316
3317 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3314.357 kb on + strand at 3314.415 kb on - strand at 3314.428 kb on - strand at 3314.502 kb on - strand at 3314.519 kb on - strand, within Dsui_3096 at 3314.520 kb on - strand, within Dsui_3096 at 3314.533 kb on + strand at 3314.652 kb on - strand, within Dsui_3096 at 3314.658 kb on - strand, within Dsui_3096 at 3314.720 kb on + strand at 3314.768 kb on + strand, within Dsui_3096 at 3314.862 kb on - strand, within Dsui_3096 at 3315.506 kb on + strand, within Dsui_3097 at 3315.531 kb on + strand at 3316.342 kb on + strand, within Dsui_3097 at 3316.342 kb on + strand, within Dsui_3097 at 3316.350 kb on - strand, within Dsui_3097 at 3316.350 kb on - strand, within Dsui_3097 at 3316.350 kb on - strand at 3316.350 kb on - strand at 3316.529 kb on + strand at 3316.534 kb on + strand at 3316.534 kb on + strand at 3316.542 kb on - strand at 3316.554 kb on - strand at 3316.601 kb on - strand at 3316.726 kb on + strand at 3316.847 kb on - strand, within Dsui_3098 at 3317.183 kb on + strand at 3317.362 kb on + strand at 3317.417 kb on + strand at 3317.425 kb on - strand at 3317.429 kb on + strand, within Dsui_3099 at 3317.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 3,314,357 + +2.3 3,314,415 - +0.3 3,314,428 - +0.7 3,314,502 - +0.3 3,314,519 - Dsui_3096 0.53 -1.1 3,314,520 - Dsui_3096 0.54 +1.7 3,314,533 + -0.5 3,314,652 - Dsui_3096 0.64 -0.4 3,314,658 - Dsui_3096 0.64 -0.4 3,314,720 + +2.3 3,314,768 + Dsui_3096 0.72 -0.5 3,314,862 - Dsui_3096 0.79 +0.6 3,315,506 + Dsui_3097 0.14 -0.7 3,315,531 + +2.3 3,316,342 + Dsui_3097 0.83 +1.0 3,316,342 + Dsui_3097 0.83 -0.5 3,316,350 - Dsui_3097 0.83 -1.5 3,316,350 - Dsui_3097 0.83 -0.7 3,316,350 - +1.3 3,316,350 - +1.3 3,316,529 + -1.9 3,316,534 + -0.7 3,316,534 + +0.6 3,316,542 - +1.3 3,316,554 - +1.9 3,316,601 - +1.7 3,316,726 + -0.2 3,316,847 - Dsui_3098 0.45 -0.3 3,317,183 + +2.3 3,317,362 + +1.3 3,317,417 + +1.7 3,317,425 - +2.3 3,317,429 + Dsui_3099 0.23 -0.9 3,317,443 + +1.3
Or see this region's nucleotide sequence