Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3013 and Dsui_3014 are separated by 9 nucleotides Dsui_3014 and Dsui_3015 overlap by 4 nucleotides Dsui_3015 and Dsui_3016 are separated by 59 nucleotides
Dsui_3013: Dsui_3013 - hypothetical protein, at 3,227,454 to 3,229,295
_3013
Dsui_3014: Dsui_3014 - outer membrane protein/peptidoglycan-associated (lipo)protein, at 3,229,305 to 3,230,024
_3014
Dsui_3015: Dsui_3015 - hypothetical protein, at 3,230,021 to 3,230,548
_3015
Dsui_3016: Dsui_3016 - hypothetical protein, at 3,230,608 to 3,230,928
_3016
Position (kb)
3229
3230
3231 Strain fitness (log2 ratio)
-1
0
1
2 at 3228.321 kb on + strand, within Dsui_3013 at 3228.353 kb on + strand, within Dsui_3013 at 3228.361 kb on - strand at 3228.507 kb on - strand at 3228.682 kb on - strand, within Dsui_3013 at 3228.789 kb on + strand, within Dsui_3013 at 3228.832 kb on - strand, within Dsui_3013 at 3229.021 kb on + strand, within Dsui_3013 at 3229.094 kb on - strand at 3229.115 kb on + strand at 3229.140 kb on + strand at 3229.181 kb on + strand at 3229.195 kb on - strand at 3229.230 kb on + strand at 3229.270 kb on - strand at 3229.290 kb on + strand at 3229.315 kb on - strand at 3229.351 kb on + strand at 3229.409 kb on - strand, within Dsui_3014 at 3229.452 kb on - strand at 3229.710 kb on + strand, within Dsui_3014 at 3229.802 kb on - strand at 3229.862 kb on + strand at 3229.870 kb on - strand at 3229.969 kb on - strand at 3229.989 kb on + strand at 3229.990 kb on - strand at 3230.050 kb on - strand at 3230.262 kb on - strand at 3230.266 kb on + strand at 3230.280 kb on - strand at 3230.400 kb on - strand at 3230.510 kb on - strand at 3230.664 kb on - strand, within Dsui_3016 at 3230.664 kb on - strand, within Dsui_3016 at 3230.670 kb on - strand, within Dsui_3016 at 3230.759 kb on - strand, within Dsui_3016 at 3230.769 kb on - strand, within Dsui_3016 at 3230.872 kb on - strand, within Dsui_3016 at 3230.952 kb on - strand at 3230.958 kb on + strand at 3230.966 kb on - strand at 3230.971 kb on - strand at 3230.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 3,228,321 + Dsui_3013 0.47 -0.1 3,228,353 + Dsui_3013 0.49 +0.4 3,228,361 - +2.3 3,228,507 - -0.5 3,228,682 - Dsui_3013 0.67 +1.6 3,228,789 + Dsui_3013 0.72 -0.3 3,228,832 - Dsui_3013 0.75 +0.3 3,229,021 + Dsui_3013 0.85 +0.3 3,229,094 - +0.3 3,229,115 + +0.4 3,229,140 + +2.3 3,229,181 + +0.0 3,229,195 - -0.5 3,229,230 + +1.0 3,229,270 - +0.1 3,229,290 + +0.0 3,229,315 - +0.3 3,229,351 + -0.1 3,229,409 - Dsui_3014 0.14 -0.0 3,229,452 - +0.7 3,229,710 + Dsui_3014 0.56 -1.1 3,229,802 - +1.3 3,229,862 + -0.0 3,229,870 - +1.3 3,229,969 - +0.3 3,229,989 + -0.9 3,229,990 - +0.3 3,230,050 - +0.5 3,230,262 - +0.5 3,230,266 + +0.3 3,230,280 - +2.3 3,230,400 - -0.0 3,230,510 - -0.5 3,230,664 - Dsui_3016 0.17 +0.2 3,230,664 - Dsui_3016 0.17 +2.3 3,230,670 - Dsui_3016 0.19 +0.0 3,230,759 - Dsui_3016 0.47 -0.1 3,230,769 - Dsui_3016 0.50 +0.5 3,230,872 - Dsui_3016 0.82 +0.5 3,230,952 - +0.7 3,230,958 + +0.4 3,230,966 - +0.2 3,230,971 - -0.0 3,230,971 - -0.3
Or see this region's nucleotide sequence