Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2603 and Dsui_2604 are separated by 358 nucleotides Dsui_2604 and Dsui_2605 are separated by 28 nucleotides Dsui_2605 and Dsui_2606 are separated by 28 nucleotides Dsui_2606 and Dsui_2607 overlap by 4 nucleotides Dsui_2607 and Dsui_2608 are separated by 22 nucleotides
Dsui_2603: Dsui_2603 - hypothetical protein, at 2,758,719 to 2,759,090
_2603
Dsui_2604: Dsui_2604 - hypothetical protein, at 2,759,449 to 2,759,643
_2604
Dsui_2605: Dsui_2605 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 2,759,672 to 2,761,408
_2605
Dsui_2606: Dsui_2606 - soluble lytic murein transglycosylase-like protein, at 2,761,437 to 2,761,913
_2606
Dsui_2607: Dsui_2607 - hypothetical protein, at 2,761,910 to 2,762,131
_2607
Dsui_2608: Dsui_2608 - topoisomerase IA, at 2,762,154 to 2,764,610
_2608
Position (kb)
2759
2760
2761
2762 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2758.674 kb on + strand at 2758.796 kb on + strand, within Dsui_2603 at 2758.817 kb on + strand at 2758.825 kb on - strand, within Dsui_2603 at 2758.876 kb on + strand, within Dsui_2603 at 2758.884 kb on - strand, within Dsui_2603 at 2758.930 kb on + strand, within Dsui_2603 at 2758.930 kb on + strand, within Dsui_2603 at 2758.938 kb on - strand, within Dsui_2603 at 2758.938 kb on - strand, within Dsui_2603 at 2758.938 kb on - strand, within Dsui_2603 at 2758.938 kb on - strand, within Dsui_2603 at 2758.938 kb on - strand, within Dsui_2603 at 2758.957 kb on + strand, within Dsui_2603 at 2758.957 kb on + strand, within Dsui_2603 at 2758.958 kb on + strand at 2758.985 kb on + strand, within Dsui_2603 at 2759.054 kb on - strand at 2759.079 kb on - strand at 2759.133 kb on - strand at 2759.222 kb on - strand at 2759.239 kb on - strand at 2759.239 kb on - strand at 2759.239 kb on - strand at 2759.368 kb on - strand at 2759.368 kb on - strand at 2759.453 kb on - strand at 2759.453 kb on - strand at 2759.482 kb on + strand at 2759.524 kb on - strand, within Dsui_2604 at 2759.544 kb on - strand, within Dsui_2604 at 2759.603 kb on - strand, within Dsui_2604 at 2759.603 kb on - strand, within Dsui_2604 at 2759.603 kb on - strand, within Dsui_2604 at 2759.613 kb on - strand, within Dsui_2604 at 2759.620 kb on - strand, within Dsui_2604 at 2759.622 kb on - strand, within Dsui_2604 at 2759.742 kb on - strand at 2759.778 kb on - strand at 2759.839 kb on + strand at 2759.839 kb on + strand at 2759.839 kb on + strand at 2759.847 kb on - strand, within Dsui_2605 at 2759.847 kb on - strand, within Dsui_2605 at 2759.847 kb on - strand, within Dsui_2605 at 2759.847 kb on - strand, within Dsui_2605 at 2759.847 kb on - strand, within Dsui_2605 at 2760.047 kb on + strand, within Dsui_2605 at 2760.101 kb on - strand, within Dsui_2605 at 2760.306 kb on + strand, within Dsui_2605 at 2760.384 kb on - strand, within Dsui_2605 at 2760.396 kb on - strand, within Dsui_2605 at 2760.407 kb on + strand, within Dsui_2605 at 2760.516 kb on + strand, within Dsui_2605 at 2760.550 kb on - strand, within Dsui_2605 at 2760.698 kb on + strand, within Dsui_2605 at 2760.712 kb on + strand, within Dsui_2605 at 2760.877 kb on + strand, within Dsui_2605 at 2760.890 kb on - strand, within Dsui_2605 at 2760.902 kb on + strand, within Dsui_2605 at 2761.005 kb on - strand, within Dsui_2605 at 2761.063 kb on + strand, within Dsui_2605 at 2761.094 kb on + strand, within Dsui_2605 at 2761.170 kb on - strand, within Dsui_2605 at 2761.249 kb on - strand at 2761.259 kb on - strand at 2761.513 kb on + strand, within Dsui_2606 at 2761.595 kb on - strand, within Dsui_2606 at 2761.634 kb on + strand, within Dsui_2606 at 2761.642 kb on - strand, within Dsui_2606 at 2761.891 kb on + strand at 2761.891 kb on + strand at 2762.067 kb on + strand, within Dsui_2607 at 2762.082 kb on + strand, within Dsui_2607 at 2762.140 kb on + strand at 2762.149 kb on - strand at 2762.163 kb on + strand at 2762.232 kb on + strand at 2762.298 kb on - strand at 2762.298 kb on - strand at 2762.379 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,758,674 + -0.3 2,758,796 + Dsui_2603 0.21 -0.2 2,758,817 + +0.5 2,758,825 - Dsui_2603 0.28 -0.2 2,758,876 + Dsui_2603 0.42 -0.1 2,758,884 - Dsui_2603 0.44 +0.0 2,758,930 + Dsui_2603 0.57 -1.2 2,758,930 + Dsui_2603 0.57 +0.0 2,758,938 - Dsui_2603 0.59 -0.6 2,758,938 - Dsui_2603 0.59 -1.5 2,758,938 - Dsui_2603 0.59 -2.1 2,758,938 - Dsui_2603 0.59 -1.1 2,758,938 - Dsui_2603 0.59 +1.3 2,758,957 + Dsui_2603 0.64 -0.4 2,758,957 + Dsui_2603 0.64 -1.2 2,758,958 + -0.5 2,758,985 + Dsui_2603 0.72 -1.5 2,759,054 - +0.7 2,759,079 - +0.2 2,759,133 - -0.1 2,759,222 - +0.5 2,759,239 - +1.3 2,759,239 - -0.4 2,759,239 - -0.5 2,759,368 - +0.6 2,759,368 - +0.0 2,759,453 - +1.5 2,759,453 - +1.9 2,759,482 + +0.2 2,759,524 - Dsui_2604 0.38 -0.1 2,759,544 - Dsui_2604 0.49 -0.7 2,759,603 - Dsui_2604 0.79 +0.2 2,759,603 - Dsui_2604 0.79 -0.7 2,759,603 - Dsui_2604 0.79 -1.6 2,759,613 - Dsui_2604 0.84 -0.8 2,759,620 - Dsui_2604 0.88 -1.4 2,759,622 - Dsui_2604 0.89 -0.3 2,759,742 - -1.0 2,759,778 - -0.4 2,759,839 + -0.1 2,759,839 + +0.5 2,759,839 + -1.1 2,759,847 - Dsui_2605 0.10 +0.1 2,759,847 - Dsui_2605 0.10 -0.8 2,759,847 - Dsui_2605 0.10 -0.3 2,759,847 - Dsui_2605 0.10 -1.5 2,759,847 - Dsui_2605 0.10 -1.0 2,760,047 + Dsui_2605 0.22 -0.8 2,760,101 - Dsui_2605 0.25 -1.2 2,760,306 + Dsui_2605 0.36 -1.0 2,760,384 - Dsui_2605 0.41 +0.2 2,760,396 - Dsui_2605 0.42 -0.3 2,760,407 + Dsui_2605 0.42 -0.3 2,760,516 + Dsui_2605 0.49 -0.7 2,760,550 - Dsui_2605 0.51 +0.7 2,760,698 + Dsui_2605 0.59 -0.9 2,760,712 + Dsui_2605 0.60 -2.8 2,760,877 + Dsui_2605 0.69 -0.3 2,760,890 - Dsui_2605 0.70 -0.7 2,760,902 + Dsui_2605 0.71 -2.1 2,761,005 - Dsui_2605 0.77 +0.0 2,761,063 + Dsui_2605 0.80 +0.3 2,761,094 + Dsui_2605 0.82 -0.1 2,761,170 - Dsui_2605 0.86 -0.4 2,761,249 - -0.7 2,761,259 - +0.7 2,761,513 + Dsui_2606 0.16 -0.3 2,761,595 - Dsui_2606 0.33 -0.5 2,761,634 + Dsui_2606 0.41 +0.3 2,761,642 - Dsui_2606 0.43 +0.2 2,761,891 + -1.2 2,761,891 + +0.4 2,762,067 + Dsui_2607 0.71 -0.2 2,762,082 + Dsui_2607 0.77 -0.4 2,762,140 + +0.0 2,762,149 - +0.7 2,762,163 + -0.0 2,762,232 + -0.3 2,762,298 - +0.1 2,762,298 - -0.3 2,762,379 + -0.4
Or see this region's nucleotide sequence