Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2487 and Dsui_2488 are separated by 7 nucleotides Dsui_2488 and Dsui_2489 are separated by 6 nucleotides Dsui_2489 and Dsui_2490 are separated by 168 nucleotides Dsui_2490 and Dsui_2491 are separated by 22 nucleotides
Dsui_2487: Dsui_2487 - hypothetical protein, at 2,636,061 to 2,637,569
_2487
Dsui_2488: Dsui_2488 - Protein of unknown function (DUF3293), at 2,637,577 to 2,638,059
_2488
Dsui_2489: Dsui_2489 - polyhydroxyalkanoate depolymerase, intracellular, at 2,638,066 to 2,639,286
_2489
Dsui_2490: Dsui_2490 - protein kinase family protein, at 2,639,455 to 2,639,880
_2490
Dsui_2491: Dsui_2491 - transposase, TnpA family, at 2,639,903 to 2,642,932
_2491
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2637.069 kb on - strand at 2637.069 kb on - strand at 2637.071 kb on - strand at 2637.115 kb on - strand, within Dsui_2487 at 2637.148 kb on - strand at 2637.148 kb on - strand at 2637.162 kb on + strand, within Dsui_2487 at 2637.193 kb on - strand at 2637.348 kb on + strand, within Dsui_2487 at 2637.348 kb on + strand, within Dsui_2487 at 2637.450 kb on + strand at 2637.505 kb on - strand at 2637.538 kb on + strand at 2637.538 kb on + strand at 2637.559 kb on + strand at 2637.698 kb on + strand, within Dsui_2488 at 2637.756 kb on + strand, within Dsui_2488 at 2637.821 kb on + strand, within Dsui_2488 at 2637.821 kb on + strand, within Dsui_2488 at 2637.915 kb on - strand, within Dsui_2488 at 2637.924 kb on + strand, within Dsui_2488 at 2638.007 kb on + strand, within Dsui_2488 at 2638.023 kb on - strand at 2638.064 kb on + strand at 2638.098 kb on + strand at 2638.098 kb on + strand at 2638.098 kb on + strand at 2638.106 kb on - strand at 2638.106 kb on - strand at 2638.106 kb on - strand at 2638.176 kb on - strand at 2638.195 kb on - strand at 2638.214 kb on - strand, within Dsui_2489 at 2638.280 kb on - strand at 2638.393 kb on + strand at 2638.462 kb on - strand, within Dsui_2489 at 2638.462 kb on - strand, within Dsui_2489 at 2638.595 kb on + strand, within Dsui_2489 at 2638.636 kb on + strand, within Dsui_2489 at 2638.639 kb on + strand at 2638.689 kb on - strand, within Dsui_2489 at 2638.696 kb on - strand, within Dsui_2489 at 2638.840 kb on + strand, within Dsui_2489 at 2638.849 kb on + strand at 2638.972 kb on + strand, within Dsui_2489 at 2639.032 kb on - strand, within Dsui_2489 at 2639.033 kb on + strand, within Dsui_2489 at 2639.150 kb on - strand, within Dsui_2489 at 2639.192 kb on + strand at 2639.447 kb on - strand at 2639.461 kb on + strand at 2639.499 kb on + strand, within Dsui_2490 at 2639.499 kb on + strand, within Dsui_2490 at 2639.499 kb on + strand, within Dsui_2490 at 2639.499 kb on + strand, within Dsui_2490 at 2639.507 kb on - strand at 2639.507 kb on - strand, within Dsui_2490 at 2639.507 kb on - strand, within Dsui_2490 at 2639.507 kb on - strand at 2639.509 kb on + strand, within Dsui_2490 at 2639.742 kb on + strand, within Dsui_2490 at 2639.748 kb on - strand, within Dsui_2490 at 2639.782 kb on + strand, within Dsui_2490 at 2639.953 kb on - strand at 2639.958 kb on - strand at 2639.975 kb on - strand at 2639.975 kb on - strand at 2639.975 kb on - strand at 2639.978 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.025 kb on + strand at 2640.026 kb on + strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.033 kb on - strand at 2640.057 kb on + strand at 2640.059 kb on - strand at 2640.089 kb on + strand at 2640.092 kb on + strand at 2640.126 kb on - strand at 2640.126 kb on - strand at 2640.163 kb on - strand at 2640.188 kb on - strand at 2640.267 kb on - strand, within Dsui_2491
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,637,069 - +0.9 2,637,069 - +2.5 2,637,071 - +0.5 2,637,115 - Dsui_2487 0.70 +0.9 2,637,148 - +1.5 2,637,148 - +2.5 2,637,162 + Dsui_2487 0.73 -0.2 2,637,193 - +0.9 2,637,348 + Dsui_2487 0.85 +0.1 2,637,348 + Dsui_2487 0.85 +1.1 2,637,450 + +0.3 2,637,505 - +1.5 2,637,538 + -0.0 2,637,538 + -0.6 2,637,559 + -0.4 2,637,698 + Dsui_2488 0.25 -0.9 2,637,756 + Dsui_2488 0.37 -0.5 2,637,821 + Dsui_2488 0.51 -0.2 2,637,821 + Dsui_2488 0.51 +0.6 2,637,915 - Dsui_2488 0.70 -0.5 2,637,924 + Dsui_2488 0.72 -0.1 2,638,007 + Dsui_2488 0.89 +0.3 2,638,023 - +0.9 2,638,064 + +0.2 2,638,098 + -0.4 2,638,098 + +1.5 2,638,098 + +0.3 2,638,106 - +1.9 2,638,106 - -1.3 2,638,106 - -0.3 2,638,176 - +2.5 2,638,195 - +2.5 2,638,214 - Dsui_2489 0.12 -0.8 2,638,280 - +0.2 2,638,393 + +0.5 2,638,462 - Dsui_2489 0.32 -0.3 2,638,462 - Dsui_2489 0.32 +0.2 2,638,595 + Dsui_2489 0.43 -0.8 2,638,636 + Dsui_2489 0.47 +0.8 2,638,639 + +0.9 2,638,689 - Dsui_2489 0.51 -0.3 2,638,696 - Dsui_2489 0.52 -0.8 2,638,840 + Dsui_2489 0.63 +0.9 2,638,849 + +0.5 2,638,972 + Dsui_2489 0.74 -0.1 2,639,032 - Dsui_2489 0.79 -0.1 2,639,033 + Dsui_2489 0.79 -0.5 2,639,150 - Dsui_2489 0.89 -0.5 2,639,192 + +1.1 2,639,447 - +0.2 2,639,461 + +1.7 2,639,499 + Dsui_2490 0.10 -0.2 2,639,499 + Dsui_2490 0.10 -0.0 2,639,499 + Dsui_2490 0.10 -0.7 2,639,499 + Dsui_2490 0.10 -0.9 2,639,507 - +1.5 2,639,507 - Dsui_2490 0.12 -0.1 2,639,507 - Dsui_2490 0.12 -1.2 2,639,507 - +0.9 2,639,509 + Dsui_2490 0.13 +0.2 2,639,742 + Dsui_2490 0.67 +0.2 2,639,748 - Dsui_2490 0.69 -0.2 2,639,782 + Dsui_2490 0.77 +0.1 2,639,953 - +0.1 2,639,958 - -2.0 2,639,975 - -0.8 2,639,975 - +1.9 2,639,975 - +0.7 2,639,978 + +0.2 2,640,025 + +0.5 2,640,025 + +0.6 2,640,025 + -1.2 2,640,025 + +0.0 2,640,025 + +0.1 2,640,025 + +0.5 2,640,025 + +1.0 2,640,025 + +0.1 2,640,025 + +0.3 2,640,026 + -0.7 2,640,033 - -0.1 2,640,033 - +0.2 2,640,033 - +1.3 2,640,033 - +1.1 2,640,033 - -1.0 2,640,033 - -0.4 2,640,033 - +0.3 2,640,033 - -0.9 2,640,057 + +1.3 2,640,059 - +0.2 2,640,089 + -0.1 2,640,092 + +0.3 2,640,126 - -0.1 2,640,126 - +0.4 2,640,163 - -0.1 2,640,188 - -0.9 2,640,267 - Dsui_2491 0.12 -0.3
Or see this region's nucleotide sequence