Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2375 and Dsui_2376 overlap by 4 nucleotides Dsui_2376 and Dsui_2377 are separated by 10 nucleotides Dsui_2377 and Dsui_2378 are separated by 76 nucleotides Dsui_2378 and Dsui_2379 are separated by 78 nucleotides
Dsui_2375: Dsui_2375 - Fe-S protein assembly chaperone HscA, at 2,524,637 to 2,526,517
_2375
Dsui_2376: Dsui_2376 - ferredoxin, 2Fe-2S type, ISC system, at 2,526,514 to 2,526,855
_2376
Dsui_2377: Dsui_2377 - FeS assembly protein IscX, at 2,526,866 to 2,527,060
_2377
Dsui_2378: Dsui_2378 - hypothetical protein, at 2,527,137 to 2,527,454
_2378
Dsui_2379: Dsui_2379 - phosphoserine phosphatase SerB, at 2,527,533 to 2,528,375
_2379
Position (kb)
2526
2527
2528 Strain fitness (log2 ratio)
-1
0
1 at 2526.849 kb on + strand at 2526.849 kb on + strand at 2526.849 kb on + strand at 2526.857 kb on - strand at 2526.976 kb on + strand, within Dsui_2377 at 2527.003 kb on - strand, within Dsui_2377 at 2527.068 kb on - strand at 2527.127 kb on + strand at 2527.146 kb on - strand at 2527.206 kb on + strand, within Dsui_2378 at 2527.310 kb on - strand, within Dsui_2378 at 2527.487 kb on + strand at 2527.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,526,849 + +0.3 2,526,849 + +0.3 2,526,849 + +0.5 2,526,857 - -0.4 2,526,976 + Dsui_2377 0.56 -0.5 2,527,003 - Dsui_2377 0.70 +0.9 2,527,068 - +0.3 2,527,127 + +0.1 2,527,146 - -1.5 2,527,206 + Dsui_2378 0.22 +0.9 2,527,310 - Dsui_2378 0.54 +0.2 2,527,487 + -0.1 2,527,509 - -0.2
Or see this region's nucleotide sequence