Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2321 and Dsui_2322 are separated by 45 nucleotides Dsui_2322 and Dsui_2323 are separated by 3 nucleotides Dsui_2323 and Dsui_2324 are separated by 54 nucleotides Dsui_2324 and Dsui_2325 are separated by 70 nucleotides
Dsui_2321: Dsui_2321 - site-specific recombinase, at 2,476,071 to 2,478,146
_2321
Dsui_2322: Dsui_2322 - hypothetical protein, at 2,478,192 to 2,478,968
_2322
Dsui_2323: Dsui_2323 - protein of unknown function (DUF1857), at 2,478,972 to 2,479,445
_2323
Dsui_2324: Dsui_2324 - hypothetical protein, at 2,479,500 to 2,479,868
_2324
Dsui_2325: Dsui_2325 - hypothetical protein, at 2,479,939 to 2,480,343
_2325
Position (kb)
2478
2479
2480 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2478.051 kb on - strand at 2478.129 kb on - strand at 2478.247 kb on + strand at 2478.377 kb on + strand, within Dsui_2322 at 2478.382 kb on + strand, within Dsui_2322 at 2478.415 kb on + strand, within Dsui_2322 at 2478.532 kb on - strand, within Dsui_2322 at 2478.568 kb on - strand, within Dsui_2322 at 2478.574 kb on - strand, within Dsui_2322 at 2478.593 kb on + strand, within Dsui_2322 at 2478.617 kb on + strand, within Dsui_2322 at 2478.625 kb on - strand at 2478.673 kb on + strand, within Dsui_2322 at 2478.682 kb on + strand, within Dsui_2322 at 2478.724 kb on - strand, within Dsui_2322 at 2478.749 kb on - strand, within Dsui_2322 at 2478.776 kb on + strand, within Dsui_2322 at 2478.837 kb on - strand at 2478.858 kb on + strand, within Dsui_2322 at 2478.877 kb on + strand, within Dsui_2322 at 2478.909 kb on - strand at 2478.956 kb on + strand at 2478.970 kb on - strand at 2479.043 kb on + strand, within Dsui_2323 at 2479.096 kb on + strand, within Dsui_2323 at 2479.108 kb on - strand at 2479.170 kb on + strand, within Dsui_2323 at 2479.228 kb on + strand, within Dsui_2323 at 2479.354 kb on + strand, within Dsui_2323 at 2479.374 kb on + strand, within Dsui_2323 at 2479.399 kb on + strand at 2479.407 kb on - strand at 2479.435 kb on + strand at 2479.570 kb on - strand, within Dsui_2324 at 2479.600 kb on + strand, within Dsui_2324 at 2479.673 kb on + strand, within Dsui_2324 at 2479.712 kb on + strand, within Dsui_2324 at 2479.753 kb on + strand, within Dsui_2324 at 2479.795 kb on - strand, within Dsui_2324 at 2479.837 kb on + strand at 2479.884 kb on - strand at 2480.024 kb on + strand at 2480.202 kb on - strand, within Dsui_2325 at 2480.207 kb on - strand, within Dsui_2325 at 2480.238 kb on - strand, within Dsui_2325 at 2480.257 kb on - strand, within Dsui_2325 at 2480.288 kb on + strand at 2480.296 kb on - strand, within Dsui_2325 at 2480.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,478,051 - -0.8 2,478,129 - +0.1 2,478,247 + -0.2 2,478,377 + Dsui_2322 0.24 +0.1 2,478,382 + Dsui_2322 0.24 -0.0 2,478,415 + Dsui_2322 0.29 -0.4 2,478,532 - Dsui_2322 0.44 -2.2 2,478,568 - Dsui_2322 0.48 -0.4 2,478,574 - Dsui_2322 0.49 -1.0 2,478,593 + Dsui_2322 0.52 -0.0 2,478,617 + Dsui_2322 0.55 -0.5 2,478,625 - +1.1 2,478,673 + Dsui_2322 0.62 -0.8 2,478,682 + Dsui_2322 0.63 -0.0 2,478,724 - Dsui_2322 0.68 +1.3 2,478,749 - Dsui_2322 0.72 -0.5 2,478,776 + Dsui_2322 0.75 -0.2 2,478,837 - +0.8 2,478,858 + Dsui_2322 0.86 +1.8 2,478,877 + Dsui_2322 0.88 +0.0 2,478,909 - -0.2 2,478,956 + -0.4 2,478,970 - +0.8 2,479,043 + Dsui_2323 0.15 +1.2 2,479,096 + Dsui_2323 0.26 -0.2 2,479,108 - +0.1 2,479,170 + Dsui_2323 0.42 +0.0 2,479,228 + Dsui_2323 0.54 +0.3 2,479,354 + Dsui_2323 0.81 +0.5 2,479,374 + Dsui_2323 0.85 +0.7 2,479,399 + -0.4 2,479,407 - +3.0 2,479,435 + +0.5 2,479,570 - Dsui_2324 0.19 -0.3 2,479,600 + Dsui_2324 0.27 +0.0 2,479,673 + Dsui_2324 0.47 +0.6 2,479,712 + Dsui_2324 0.57 -1.1 2,479,753 + Dsui_2324 0.69 +0.4 2,479,795 - Dsui_2324 0.80 -0.6 2,479,837 + +0.2 2,479,884 - -0.3 2,480,024 + +1.4 2,480,202 - Dsui_2325 0.65 -0.1 2,480,207 - Dsui_2325 0.66 -0.1 2,480,238 - Dsui_2325 0.74 -0.5 2,480,257 - Dsui_2325 0.79 +0.3 2,480,288 + +0.4 2,480,296 - Dsui_2325 0.88 -0.2 2,480,419 - -0.8
Or see this region's nucleotide sequence