Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2269 and Dsui_2270 are separated by 280 nucleotides Dsui_2270 and Dsui_2271 are separated by 54 nucleotides Dsui_2271 and Dsui_2272 are separated by 215 nucleotides
Dsui_2269: Dsui_2269 - SOS regulatory protein LexA, at 2,420,598 to 2,421,209
_2269
Dsui_2270: Dsui_2270 - hypothetical protein, at 2,421,490 to 2,421,693
_2270
Dsui_2271: Dsui_2271 - hypothetical protein, at 2,421,748 to 2,422,731
_2271
Dsui_2272: Dsui_2272 - hypothetical protein, at 2,422,947 to 2,424,431
_2272
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2420.959 kb on - strand, within Dsui_2269 at 2421.207 kb on - strand at 2421.440 kb on + strand at 2421.487 kb on + strand at 2421.605 kb on - strand, within Dsui_2270 at 2421.618 kb on - strand at 2421.902 kb on - strand, within Dsui_2271 at 2422.011 kb on + strand at 2422.050 kb on + strand, within Dsui_2271 at 2422.066 kb on - strand, within Dsui_2271 at 2422.133 kb on - strand, within Dsui_2271 at 2422.287 kb on + strand, within Dsui_2271 at 2422.302 kb on + strand, within Dsui_2271 at 2422.310 kb on - strand, within Dsui_2271 at 2422.339 kb on + strand at 2422.417 kb on + strand at 2422.453 kb on - strand, within Dsui_2271 at 2422.559 kb on - strand, within Dsui_2271 at 2422.574 kb on + strand at 2422.611 kb on + strand at 2422.637 kb on + strand at 2422.730 kb on - strand at 2422.741 kb on + strand at 2422.776 kb on + strand at 2422.950 kb on + strand at 2423.020 kb on - strand at 2423.159 kb on + strand at 2423.167 kb on - strand, within Dsui_2272 at 2423.173 kb on - strand at 2423.244 kb on - strand, within Dsui_2272 at 2423.263 kb on - strand, within Dsui_2272 at 2423.279 kb on - strand, within Dsui_2272 at 2423.561 kb on + strand at 2423.651 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,420,959 - Dsui_2269 0.59 -0.0 2,421,207 - -1.2 2,421,440 + +1.4 2,421,487 + +2.4 2,421,605 - Dsui_2270 0.56 +0.6 2,421,618 - +0.8 2,421,902 - Dsui_2271 0.16 -1.0 2,422,011 + +1.4 2,422,050 + Dsui_2271 0.31 -0.0 2,422,066 - Dsui_2271 0.32 -0.3 2,422,133 - Dsui_2271 0.39 +2.4 2,422,287 + Dsui_2271 0.55 -0.1 2,422,302 + Dsui_2271 0.56 -1.1 2,422,310 - Dsui_2271 0.57 -1.3 2,422,339 + +0.4 2,422,417 + +1.1 2,422,453 - Dsui_2271 0.72 -0.1 2,422,559 - Dsui_2271 0.82 +0.8 2,422,574 + +2.4 2,422,611 + +2.4 2,422,637 + -1.2 2,422,730 - +0.7 2,422,741 + +0.1 2,422,776 + +1.4 2,422,950 + +1.4 2,423,020 - -0.4 2,423,159 + +0.8 2,423,167 - Dsui_2272 0.15 +0.5 2,423,173 - +0.8 2,423,244 - Dsui_2272 0.20 +0.2 2,423,263 - Dsui_2272 0.21 -1.5 2,423,279 - Dsui_2272 0.22 +1.1 2,423,561 + +0.1 2,423,651 + +3.4
Or see this region's nucleotide sequence