Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1426 and Dsui_1427 are separated by 20 nucleotides Dsui_1427 and Dsui_1428 are separated by 19 nucleotides Dsui_1428 and Dsui_1429 are separated by 154 nucleotides
Dsui_1426: Dsui_1426 - periplasmic serine protease, Do/DeqQ family, at 1,478,260 to 1,479,414
_1426
Dsui_1427: Dsui_1427 - dinuclear metal center protein, YbgI/SA1388 family, at 1,479,435 to 1,480,181
_1427
Dsui_1428: Dsui_1428 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase, at 1,480,201 to 1,480,917
_1428
Dsui_1429: Dsui_1429 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,481,072 to 1,484,548
_1429
Position (kb)
1479
1480
1481 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1478.517 kb on + strand, within Dsui_1426 at 1478.747 kb on + strand, within Dsui_1426 at 1478.801 kb on - strand, within Dsui_1426 at 1478.846 kb on + strand at 1478.938 kb on - strand, within Dsui_1426 at 1479.096 kb on + strand, within Dsui_1426 at 1479.096 kb on + strand, within Dsui_1426 at 1479.104 kb on - strand, within Dsui_1426 at 1479.214 kb on - strand, within Dsui_1426 at 1479.215 kb on + strand, within Dsui_1426 at 1479.335 kb on + strand at 1479.344 kb on - strand at 1479.368 kb on + strand at 1479.375 kb on + strand at 1479.473 kb on + strand at 1479.575 kb on - strand at 1479.685 kb on - strand at 1479.685 kb on - strand, within Dsui_1427 at 1479.696 kb on - strand, within Dsui_1427 at 1479.696 kb on - strand at 1479.700 kb on - strand, within Dsui_1427 at 1479.710 kb on + strand, within Dsui_1427 at 1479.784 kb on - strand at 1479.965 kb on + strand, within Dsui_1427 at 1479.990 kb on - strand, within Dsui_1427 at 1480.046 kb on - strand at 1480.183 kb on - strand at 1480.234 kb on + strand at 1480.270 kb on - strand at 1480.427 kb on + strand, within Dsui_1428 at 1480.453 kb on - strand, within Dsui_1428 at 1480.524 kb on + strand, within Dsui_1428 at 1480.530 kb on - strand, within Dsui_1428 at 1480.546 kb on + strand, within Dsui_1428 at 1480.625 kb on - strand, within Dsui_1428 at 1480.632 kb on - strand, within Dsui_1428 at 1480.633 kb on - strand, within Dsui_1428 at 1480.635 kb on + strand, within Dsui_1428 at 1480.852 kb on - strand at 1480.960 kb on + strand at 1481.026 kb on + strand at 1481.093 kb on + strand at 1481.129 kb on + strand at 1481.129 kb on + strand at 1481.134 kb on + strand at 1481.134 kb on + strand at 1481.142 kb on - strand at 1481.147 kb on - strand at 1481.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,478,517 + Dsui_1426 0.22 +1.3 1,478,747 + Dsui_1426 0.42 +0.0 1,478,801 - Dsui_1426 0.47 +2.5 1,478,846 + +1.5 1,478,938 - Dsui_1426 0.59 -1.3 1,479,096 + Dsui_1426 0.72 -1.9 1,479,096 + Dsui_1426 0.72 +0.3 1,479,104 - Dsui_1426 0.73 -0.7 1,479,214 - Dsui_1426 0.83 -0.1 1,479,215 + Dsui_1426 0.83 -0.1 1,479,335 + -0.3 1,479,344 - -0.1 1,479,368 + +0.0 1,479,375 + -0.7 1,479,473 + +0.9 1,479,575 - +1.5 1,479,685 - +1.5 1,479,685 - Dsui_1427 0.33 +1.5 1,479,696 - Dsui_1427 0.35 +0.2 1,479,696 - +1.5 1,479,700 - Dsui_1427 0.35 +1.7 1,479,710 + Dsui_1427 0.37 -0.3 1,479,784 - +0.5 1,479,965 + Dsui_1427 0.71 +0.6 1,479,990 - Dsui_1427 0.74 +0.4 1,480,046 - +2.5 1,480,183 - +0.2 1,480,234 + -0.2 1,480,270 - -0.3 1,480,427 + Dsui_1428 0.32 +0.4 1,480,453 - Dsui_1428 0.35 +1.1 1,480,524 + Dsui_1428 0.45 +0.6 1,480,530 - Dsui_1428 0.46 -0.3 1,480,546 + Dsui_1428 0.48 -0.7 1,480,625 - Dsui_1428 0.59 -0.6 1,480,632 - Dsui_1428 0.60 +0.5 1,480,633 - Dsui_1428 0.60 -0.5 1,480,635 + Dsui_1428 0.61 -0.5 1,480,852 - +0.5 1,480,960 + -0.6 1,481,026 + -1.3 1,481,093 + -1.7 1,481,129 + -0.2 1,481,129 + -0.8 1,481,134 + +0.2 1,481,134 + +0.3 1,481,142 - -0.2 1,481,147 - +0.1 1,481,164 + -2.3
Or see this region's nucleotide sequence