Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1392 and Dsui_1393 are separated by 9 nucleotides Dsui_1393 and Dsui_1394 overlap by 4 nucleotides
Dsui_1392: Dsui_1392 - type II secretory pathway, component PulF, at 1,449,645 to 1,450,844
_1392
Dsui_1393: Dsui_1393 - type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 1,450,854 to 1,452,560
_1393
Dsui_1394: Dsui_1394 - hypothetical protein, at 1,452,557 to 1,453,534
_1394
Position (kb)
1450
1451
1452
1453 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1449.906 kb on + strand, within Dsui_1392 at 1449.975 kb on - strand at 1450.036 kb on - strand, within Dsui_1392 at 1450.236 kb on + strand, within Dsui_1392 at 1450.244 kb on - strand, within Dsui_1392 at 1450.292 kb on + strand, within Dsui_1392 at 1450.328 kb on - strand, within Dsui_1392 at 1450.457 kb on + strand, within Dsui_1392 at 1450.476 kb on - strand, within Dsui_1392 at 1450.479 kb on + strand, within Dsui_1392 at 1450.541 kb on + strand at 1450.545 kb on - strand at 1450.697 kb on + strand at 1450.719 kb on + strand at 1450.720 kb on + strand, within Dsui_1392 at 1450.727 kb on - strand at 1450.853 kb on - strand at 1450.857 kb on + strand at 1450.872 kb on + strand at 1450.901 kb on + strand at 1450.909 kb on - strand at 1450.988 kb on - strand at 1451.348 kb on + strand at 1451.399 kb on - strand at 1451.639 kb on + strand at 1451.639 kb on + strand, within Dsui_1393 at 1451.639 kb on + strand at 1451.647 kb on - strand at 1451.656 kb on + strand at 1451.668 kb on + strand, within Dsui_1393 at 1451.668 kb on - strand, within Dsui_1393 at 1451.932 kb on + strand, within Dsui_1393 at 1452.105 kb on - strand at 1452.112 kb on - strand, within Dsui_1393 at 1452.278 kb on - strand at 1452.462 kb on - strand at 1452.661 kb on - strand, within Dsui_1394 at 1452.784 kb on - strand, within Dsui_1394 at 1452.797 kb on + strand, within Dsui_1394 at 1452.830 kb on - strand, within Dsui_1394 at 1452.892 kb on - strand, within Dsui_1394 at 1453.053 kb on - strand at 1453.103 kb on - strand, within Dsui_1394 at 1453.431 kb on - strand, within Dsui_1394 at 1453.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,449,906 + Dsui_1392 0.22 -0.1 1,449,975 - +0.9 1,450,036 - Dsui_1392 0.33 -0.6 1,450,236 + Dsui_1392 0.49 -0.1 1,450,244 - Dsui_1392 0.50 -2.2 1,450,292 + Dsui_1392 0.54 -2.1 1,450,328 - Dsui_1392 0.57 +1.3 1,450,457 + Dsui_1392 0.68 +0.4 1,450,476 - Dsui_1392 0.69 -0.3 1,450,479 + Dsui_1392 0.69 +0.8 1,450,541 + +0.5 1,450,545 - +2.5 1,450,697 + +1.5 1,450,719 + +0.9 1,450,720 + Dsui_1392 0.90 -1.1 1,450,727 - -1.8 1,450,853 - +3.5 1,450,857 + -0.1 1,450,872 + +1.9 1,450,901 + -0.5 1,450,909 - -2.2 1,450,988 - +0.0 1,451,348 + +2.5 1,451,399 - +0.5 1,451,639 + +0.9 1,451,639 + Dsui_1393 0.46 +0.1 1,451,639 + -0.1 1,451,647 - +0.5 1,451,656 + +1.2 1,451,668 + Dsui_1393 0.48 -0.6 1,451,668 - Dsui_1393 0.48 -2.2 1,451,932 + Dsui_1393 0.63 +0.0 1,452,105 - +0.5 1,452,112 - Dsui_1393 0.74 -0.9 1,452,278 - +1.5 1,452,462 - +0.6 1,452,661 - Dsui_1394 0.11 -0.8 1,452,784 - Dsui_1394 0.23 +0.1 1,452,797 + Dsui_1394 0.25 +0.6 1,452,830 - Dsui_1394 0.28 +0.1 1,452,892 - Dsui_1394 0.34 +0.1 1,453,053 - +0.9 1,453,103 - Dsui_1394 0.56 +0.5 1,453,431 - Dsui_1394 0.89 -0.6 1,453,468 - -0.1
Or see this region's nucleotide sequence