Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0795 and Dsui_0796 are separated by 76 nucleotides Dsui_0796 and Dsui_0797 are separated by 9 nucleotides Dsui_0797 and Dsui_0798 are separated by 163 nucleotides Dsui_0798 and Dsui_0799 are separated by 174 nucleotides
Dsui_0795: Dsui_0795 - putative enzyme of the cupin superfamily, at 802,655 to 803,020
_0795
Dsui_0796: Dsui_0796 - hypothetical protein, at 803,097 to 803,474
_0796
Dsui_0797: Dsui_0797 - protein of unknown function (DUF1840), at 803,484 to 803,816
_0797
Dsui_0798: Dsui_0798 - carbonic anhydrase, at 803,980 to 804,636
_0798
Dsui_0799: Dsui_0799 - succinate CoA transferase, at 804,811 to 806,370
_0799
Position (kb)
803
804
805 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 803.011 kb on + strand at 803.096 kb on - strand at 803.129 kb on + strand at 803.129 kb on + strand at 803.129 kb on + strand at 803.133 kb on - strand at 803.142 kb on + strand, within Dsui_0796 at 803.314 kb on + strand, within Dsui_0796 at 803.314 kb on + strand, within Dsui_0796 at 803.322 kb on - strand, within Dsui_0796 at 803.334 kb on + strand, within Dsui_0796 at 803.478 kb on - strand at 803.478 kb on - strand at 803.483 kb on - strand at 803.518 kb on - strand at 803.573 kb on - strand, within Dsui_0797 at 803.622 kb on - strand, within Dsui_0797 at 803.681 kb on + strand, within Dsui_0797 at 803.857 kb on - strand at 803.876 kb on - strand at 803.889 kb on - strand at 803.913 kb on + strand at 803.921 kb on - strand at 803.923 kb on + strand at 803.923 kb on + strand at 803.931 kb on + strand at 804.007 kb on + strand at 804.012 kb on + strand at 804.012 kb on + strand at 804.017 kb on + strand at 804.032 kb on - strand at 804.081 kb on - strand, within Dsui_0798 at 804.150 kb on + strand, within Dsui_0798 at 804.198 kb on - strand, within Dsui_0798 at 804.212 kb on + strand, within Dsui_0798 at 804.222 kb on - strand, within Dsui_0798 at 804.233 kb on - strand, within Dsui_0798 at 804.258 kb on + strand, within Dsui_0798 at 804.264 kb on + strand, within Dsui_0798 at 804.301 kb on + strand, within Dsui_0798 at 804.312 kb on + strand, within Dsui_0798 at 804.320 kb on - strand, within Dsui_0798 at 804.328 kb on + strand, within Dsui_0798 at 804.348 kb on + strand, within Dsui_0798 at 804.381 kb on + strand, within Dsui_0798 at 804.389 kb on - strand, within Dsui_0798 at 804.437 kb on - strand, within Dsui_0798 at 804.493 kb on - strand, within Dsui_0798 at 804.504 kb on - strand, within Dsui_0798 at 804.519 kb on - strand, within Dsui_0798 at 804.530 kb on - strand, within Dsui_0798 at 804.530 kb on - strand, within Dsui_0798 at 804.535 kb on + strand, within Dsui_0798 at 804.572 kb on + strand at 804.635 kb on + strand at 804.771 kb on - strand at 804.810 kb on - strand at 804.838 kb on + strand at 804.860 kb on + strand at 804.928 kb on + strand at 804.933 kb on + strand at 804.933 kb on + strand at 804.941 kb on - strand at 804.966 kb on + strand at 805.031 kb on + strand, within Dsui_0799 at 805.104 kb on - strand, within Dsui_0799 at 805.196 kb on + strand, within Dsui_0799 at 805.196 kb on + strand, within Dsui_0799 at 805.196 kb on + strand, within Dsui_0799 at 805.204 kb on - strand, within Dsui_0799 at 805.208 kb on - strand at 805.239 kb on + strand, within Dsui_0799 at 805.272 kb on + strand, within Dsui_0799 at 805.315 kb on + strand, within Dsui_0799 at 805.386 kb on + strand, within Dsui_0799 at 805.501 kb on + strand, within Dsui_0799 at 805.509 kb on - strand, within Dsui_0799 at 805.526 kb on - strand, within Dsui_0799 at 805.564 kb on - strand, within Dsui_0799
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 803,011 + +0.4 803,096 - -1.3 803,129 + -0.4 803,129 + +0.3 803,129 + +0.4 803,133 - +0.1 803,142 + Dsui_0796 0.12 +0.2 803,314 + Dsui_0796 0.57 -0.6 803,314 + Dsui_0796 0.57 +1.2 803,322 - Dsui_0796 0.60 -1.6 803,334 + Dsui_0796 0.63 -3.5 803,478 - +0.1 803,478 - -0.4 803,483 - +0.3 803,518 - +0.1 803,573 - Dsui_0797 0.27 -0.7 803,622 - Dsui_0797 0.41 +0.5 803,681 + Dsui_0797 0.59 +0.8 803,857 - +0.8 803,876 - +1.1 803,889 - +0.1 803,913 + +0.8 803,921 - +0.8 803,923 + -0.4 803,923 + -0.4 803,931 + +0.3 804,007 + -1.6 804,012 + -0.3 804,012 + -0.7 804,017 + +0.6 804,032 - +0.7 804,081 - Dsui_0798 0.15 +1.6 804,150 + Dsui_0798 0.26 -0.1 804,198 - Dsui_0798 0.33 +0.2 804,212 + Dsui_0798 0.35 +1.3 804,222 - Dsui_0798 0.37 +1.1 804,233 - Dsui_0798 0.39 +0.2 804,258 + Dsui_0798 0.42 -0.1 804,264 + Dsui_0798 0.43 -0.2 804,301 + Dsui_0798 0.49 -0.3 804,312 + Dsui_0798 0.51 +0.5 804,320 - Dsui_0798 0.52 +0.2 804,328 + Dsui_0798 0.53 +0.9 804,348 + Dsui_0798 0.56 +0.1 804,381 + Dsui_0798 0.61 +1.2 804,389 - Dsui_0798 0.62 -0.2 804,437 - Dsui_0798 0.70 +0.3 804,493 - Dsui_0798 0.78 +1.1 804,504 - Dsui_0798 0.80 +1.0 804,519 - Dsui_0798 0.82 -1.0 804,530 - Dsui_0798 0.84 +0.0 804,530 - Dsui_0798 0.84 -0.4 804,535 + Dsui_0798 0.84 -0.8 804,572 + -0.3 804,635 + +0.4 804,771 - -0.2 804,810 - -0.4 804,838 + -1.4 804,860 + -0.9 804,928 + -0.9 804,933 + -0.6 804,933 + -0.6 804,941 - -1.3 804,966 + +0.5 805,031 + Dsui_0799 0.14 +0.6 805,104 - Dsui_0799 0.19 -0.4 805,196 + Dsui_0799 0.25 +1.1 805,196 + Dsui_0799 0.25 +0.1 805,196 + Dsui_0799 0.25 +0.2 805,204 - Dsui_0799 0.25 -0.3 805,208 - +0.1 805,239 + Dsui_0799 0.27 -0.4 805,272 + Dsui_0799 0.30 +0.1 805,315 + Dsui_0799 0.32 -0.0 805,386 + Dsui_0799 0.37 -1.0 805,501 + Dsui_0799 0.44 -0.2 805,509 - Dsui_0799 0.45 -1.0 805,526 - Dsui_0799 0.46 +0.4 805,564 - Dsui_0799 0.48 +0.8
Or see this region's nucleotide sequence