Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0186 and Dsui_0187 are separated by 16 nucleotides Dsui_0187 and Dsui_0188 are separated by 28 nucleotides Dsui_0188 and Dsui_0189 are separated by 181 nucleotides Dsui_0189 and Dsui_0190 overlap by 4 nucleotides Dsui_0190 and Dsui_0191 overlap by 23 nucleotides
Dsui_0186: Dsui_0186 - DNA/RNA helicase, superfamily II, SNF2 family, at 185,870 to 188,398
_0186
Dsui_0187: Dsui_0187 - hypothetical protein, at 188,415 to 189,269
_0187
Dsui_0188: Dsui_0188 - hypothetical protein, at 189,298 to 189,816
_0188
Dsui_0189: Dsui_0189 - holliday junction resolvase, at 189,998 to 190,438
_0189
Dsui_0190: Dsui_0190 - hypothetical protein, at 190,435 to 190,635
_0190
Dsui_0191: Dsui_0191 - hypothetical protein, at 190,613 to 191,242
_0191
Position (kb) 189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 188.391 kb on - strand at 188.410 kb on - strand at 188.421 kb on - strand at 188.463 kb on + strand at 188.463 kb on + strand at 188.481 kb on - strand at 188.673 kb on + strand, within Dsui_0187 at 188.707 kb on - strand, within Dsui_0187 at 188.731 kb on - strand, within Dsui_0187 at 188.859 kb on - strand, within Dsui_0187 at 188.936 kb on - strand, within Dsui_0187 at 188.978 kb on - strand, within Dsui_0187 at 188.986 kb on + strand, within Dsui_0187 at 189.032 kb on - strand, within Dsui_0187 at 189.071 kb on + strand, within Dsui_0187 at 189.114 kb on + strand, within Dsui_0187 at 189.138 kb on - strand, within Dsui_0187 at 189.316 kb on + strand at 189.539 kb on + strand at 189.573 kb on + strand, within Dsui_0188 at 189.596 kb on + strand, within Dsui_0188 at 189.741 kb on - strand, within Dsui_0188 at 189.745 kb on - strand, within Dsui_0188 at 189.823 kb on + strand at 189.823 kb on + strand at 189.902 kb on - strand at 189.966 kb on + strand at 189.977 kb on + strand at 190.057 kb on - strand, within Dsui_0189 at 190.101 kb on - strand, within Dsui_0189 at 190.153 kb on + strand, within Dsui_0189 at 190.153 kb on + strand, within Dsui_0189 at 190.153 kb on + strand, within Dsui_0189 at 190.161 kb on - strand, within Dsui_0189 at 190.161 kb on - strand, within Dsui_0189 at 190.198 kb on + strand, within Dsui_0189 at 190.198 kb on + strand, within Dsui_0189 at 190.212 kb on - strand, within Dsui_0189 at 190.279 kb on + strand, within Dsui_0189 at 190.367 kb on - strand, within Dsui_0189 at 190.432 kb on + strand at 190.546 kb on + strand, within Dsui_0190 at 190.651 kb on + strand at 190.668 kb on + strand at 190.697 kb on + strand, within Dsui_0191 at 190.705 kb on - strand, within Dsui_0191 at 190.792 kb on - strand, within Dsui_0191 at 190.797 kb on - strand, within Dsui_0191 at 190.806 kb on - strand, within Dsui_0191
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 188,391 - +1.6 188,410 - -3.5 188,421 - +0.0 188,463 + +0.5 188,463 + -0.6 188,481 - -0.9 188,673 + Dsui_0187 0.30 -0.1 188,707 - Dsui_0187 0.34 -0.9 188,731 - Dsui_0187 0.37 -1.0 188,859 - Dsui_0187 0.52 +0.4 188,936 - Dsui_0187 0.61 -1.2 188,978 - Dsui_0187 0.66 -0.3 188,986 + Dsui_0187 0.67 -0.9 189,032 - Dsui_0187 0.72 -0.2 189,071 + Dsui_0187 0.77 +0.3 189,114 + Dsui_0187 0.82 -0.3 189,138 - Dsui_0187 0.85 +0.2 189,316 + -0.6 189,539 + +0.6 189,573 + Dsui_0188 0.53 -0.1 189,596 + Dsui_0188 0.57 -0.8 189,741 - Dsui_0188 0.85 -1.8 189,745 - Dsui_0188 0.86 +0.3 189,823 + -0.0 189,823 + +1.0 189,902 - +0.7 189,966 + +0.3 189,977 + -0.5 190,057 - Dsui_0189 0.13 -0.3 190,101 - Dsui_0189 0.23 +0.6 190,153 + Dsui_0189 0.35 -1.1 190,153 + Dsui_0189 0.35 -1.1 190,153 + Dsui_0189 0.35 -0.8 190,161 - Dsui_0189 0.37 -0.6 190,161 - Dsui_0189 0.37 -0.1 190,198 + Dsui_0189 0.45 +1.1 190,198 + Dsui_0189 0.45 -0.2 190,212 - Dsui_0189 0.49 +0.3 190,279 + Dsui_0189 0.64 -0.0 190,367 - Dsui_0189 0.84 -0.3 190,432 + +0.2 190,546 + Dsui_0190 0.55 -1.3 190,651 + -0.9 190,668 + -0.2 190,697 + Dsui_0191 0.13 -0.4 190,705 - Dsui_0191 0.15 +1.6 190,792 - Dsui_0191 0.28 +0.9 190,797 - Dsui_0191 0.29 -0.9 190,806 - Dsui_0191 0.31 -1.9
Or see this region's nucleotide sequence