Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0030 and Dsui_0031 are separated by 129 nucleotides Dsui_0031 and Dsui_0032 are separated by 91 nucleotides Dsui_0032 and Dsui_0033 are separated by 90 nucleotides
Dsui_0030: Dsui_0030 - FtsH-interacting integral membrane protein, at 27,028 to 27,720
_0030
Dsui_0031: Dsui_0031 - cation/cationic drug transporter, at 27,850 to 28,173
_0031
Dsui_0032: Dsui_0032 - NAD-dependent protein deacetylase, SIR2 family, at 28,265 to 29,095
_0032
Dsui_0033: Dsui_0033 - Zn-dependent protease with chaperone function, at 29,186 to 30,007
_0033
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 27.306 kb on + strand at 27.343 kb on + strand at 27.385 kb on + strand at 27.750 kb on + strand at 27.840 kb on + strand at 27.964 kb on + strand, within Dsui_0031 at 28.036 kb on + strand, within Dsui_0031 at 28.078 kb on + strand, within Dsui_0031 at 28.106 kb on - strand at 28.159 kb on - strand at 28.225 kb on + strand at 28.273 kb on + strand at 28.284 kb on + strand at 28.326 kb on - strand at 28.350 kb on + strand at 28.398 kb on + strand, within Dsui_0032 at 28.474 kb on + strand, within Dsui_0032 at 28.477 kb on - strand at 28.685 kb on - strand at 28.694 kb on + strand at 28.893 kb on + strand, within Dsui_0032 at 28.967 kb on + strand at 28.975 kb on - strand, within Dsui_0032 at 28.999 kb on + strand at 29.007 kb on - strand, within Dsui_0032 at 29.007 kb on - strand at 29.024 kb on - strand at 29.107 kb on - strand at 29.107 kb on - strand at 29.157 kb on - strand at 29.280 kb on - strand at 29.299 kb on + strand, within Dsui_0033 at 29.458 kb on + strand, within Dsui_0033 at 29.507 kb on - strand at 29.600 kb on + strand, within Dsui_0033 at 29.718 kb on + strand, within Dsui_0033 at 29.864 kb on + strand, within Dsui_0033 at 29.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 27,306 + +1.6 27,343 + +2.6 27,385 + +1.0 27,750 + -0.4 27,840 + +0.7 27,964 + Dsui_0031 0.35 +1.2 28,036 + Dsui_0031 0.57 -0.7 28,078 + Dsui_0031 0.70 -0.2 28,106 - +0.6 28,159 - +1.0 28,225 + +2.0 28,273 + +0.6 28,284 + +1.0 28,326 - +2.6 28,350 + +2.0 28,398 + Dsui_0032 0.16 -0.7 28,474 + Dsui_0032 0.25 +0.3 28,477 - +0.6 28,685 - +1.6 28,694 + +0.6 28,893 + Dsui_0032 0.76 -1.1 28,967 + +1.0 28,975 - Dsui_0032 0.85 -0.0 28,999 + +1.0 29,007 - Dsui_0032 0.89 -0.2 29,007 - +1.6 29,024 - +4.2 29,107 - +0.3 29,107 - +1.0 29,157 - +1.0 29,280 - +0.3 29,299 + Dsui_0033 0.14 -0.7 29,458 + Dsui_0033 0.33 +0.2 29,507 - +2.6 29,600 + Dsui_0033 0.50 -0.1 29,718 + Dsui_0033 0.65 -0.5 29,864 + Dsui_0033 0.82 -0.3 29,951 - +1.2
Or see this region's nucleotide sequence