Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Met-4 and tRNA-Met-3 are separated by 147 nucleotides tRNA-Met-3 and tRNA-Met-2 are separated by 147 nucleotides tRNA-Met-2 and tRNA-Met-1 are separated by 37 nucleotides tRNA-Met-1 and SO1851 are separated by 257 nucleotides
SO_t027: tRNA-Met-4 - tRNA-Met (RefSeq), at 1,939,976 to 1,940,052
tRNA-Met-4
SO_t028: tRNA-Met-3 - tRNA-Met (RefSeq), at 1,940,200 to 1,940,276
tRNA-Met-3
SO_t029: tRNA-Met-2 - tRNA-Met (RefSeq), at 1,940,424 to 1,940,500
tRNA-Met-2
SO_t030: tRNA-Met-1 - tRNA-Met (RefSeq), at 1,940,538 to 1,940,614
tRNA-Met-1
SO1851: SO1851 - conserved hypothetical protein (NCBI ptt file), at 1,940,872 to 1,943,007
SO1851
Position (kb)
1940
1941 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1940.152 kb on + strand at 1940.223 kb on - strand, within tRNA-Met-3 at 1940.339 kb on + strand at 1940.635 kb on + strand at 1940.643 kb on - strand at 1940.854 kb on - strand at 1940.989 kb on + strand at 1940.989 kb on + strand at 1940.991 kb on + strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1941.008 kb on + strand at 1941.008 kb on + strand at 1941.008 kb on + strand at 1941.016 kb on - strand at 1941.025 kb on + strand at 1941.025 kb on + strand at 1941.038 kb on + strand at 1941.046 kb on - strand at 1941.104 kb on + strand, within SO1851 at 1941.243 kb on - strand, within SO1851 at 1941.256 kb on + strand, within SO1851 at 1941.318 kb on - strand, within SO1851 at 1941.363 kb on + strand, within SO1851 at 1941.417 kb on + strand, within SO1851 at 1941.427 kb on + strand, within SO1851 at 1941.476 kb on + strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.489 kb on - strand, within SO1851
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 1,940,152 + +1.1 1,940,223 - tRNA-Met-3 SO_t028 0.30 +0.2 1,940,339 + -1.6 1,940,635 + -1.8 1,940,643 - -2.3 1,940,854 - +0.0 1,940,989 + -0.9 1,940,989 + -0.1 1,940,991 + -0.9 1,940,997 - +0.2 1,940,997 - -1.8 1,940,997 - -0.1 1,940,997 - -2.8 1,940,997 - -0.8 1,941,008 + -0.3 1,941,008 + -2.4 1,941,008 + -1.3 1,941,016 - -0.1 1,941,025 + -1.6 1,941,025 + -0.0 1,941,038 + +0.4 1,941,046 - -0.3 1,941,104 + SO1851 0.11 -1.5 1,941,243 - SO1851 0.17 -1.0 1,941,256 + SO1851 0.18 -0.1 1,941,318 - SO1851 0.21 -3.3 1,941,363 + SO1851 0.23 -1.3 1,941,417 + SO1851 0.26 -1.8 1,941,427 + SO1851 0.26 +0.3 1,941,476 + SO1851 0.28 -0.2 1,941,484 - SO1851 0.29 -0.7 1,941,484 - SO1851 0.29 -1.5 1,941,484 - SO1851 0.29 -1.0 1,941,489 - SO1851 0.29 -1.4
Or see this region's nucleotide sequence