Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2499 and SO2500 are separated by 163 nucleotides SO2500 and tRNA-Tyr-4 are separated by 125 nucleotides tRNA-Tyr-4 and tRNA-Tyr-3 are separated by 39 nucleotides tRNA-Tyr-3 and tRNA-Tyr-2 are separated by 117 nucleotides tRNA-Tyr-2 and SO2501 are separated by 206 nucleotides
SO2499: SO2499 - conserved hypothetical protein (NCBI ptt file), at 2,625,423 to 2,625,710
SO2499
SO2500: SO2500 - conserved hypothetical protein (NCBI ptt file), at 2,625,874 to 2,626,362
SO2500
SO_t045: tRNA-Tyr-4 - tRNA-Tyr (RefSeq), at 2,626,488 to 2,626,572
tRNA-Tyr-4
SO_t046: tRNA-Tyr-3 - tRNA-Tyr (RefSeq), at 2,626,612 to 2,626,696
tRNA-Tyr-3
SO_t047: tRNA-Tyr-2 - tRNA-Tyr (RefSeq), at 2,626,814 to 2,626,898
tRNA-Tyr-2
SO2501: SO2501 - radical activating enzyme (NCBI ptt file), at 2,627,105 to 2,627,773
SO2501
Position (kb)
2626
2627 Strain fitness (log2 ratio)
-1
0
1 at 2625.712 kb on + strand at 2625.817 kb on - strand at 2625.858 kb on + strand at 2625.881 kb on - strand at 2625.883 kb on + strand at 2625.883 kb on + strand at 2625.980 kb on - strand, within SO2500 at 2625.983 kb on + strand, within SO2500 at 2625.999 kb on - strand, within SO2500 at 2626.000 kb on + strand, within SO2500 at 2626.064 kb on + strand, within SO2500 at 2627.101 kb on - strand at 2627.134 kb on + strand at 2627.210 kb on - strand, within SO2501 at 2627.217 kb on - strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.315 kb on + strand, within SO2501 at 2627.323 kb on - strand, within SO2501 at 2627.450 kb on + strand, within SO2501 at 2627.520 kb on + strand, within SO2501 at 2627.520 kb on + strand, within SO2501 at 2627.528 kb on - strand, within SO2501 at 2627.530 kb on - strand, within SO2501 at 2627.590 kb on + strand, within SO2501 at 2627.598 kb on - strand, within SO2501 at 2627.646 kb on - strand, within SO2501 at 2627.674 kb on + strand, within SO2501
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,625,712 + +0.4 2,625,817 - +0.1 2,625,858 + +0.6 2,625,881 - +0.5 2,625,883 + -1.2 2,625,883 + -0.1 2,625,980 - SO2500 0.22 -0.8 2,625,983 + SO2500 0.22 -0.5 2,625,999 - SO2500 0.26 +0.4 2,626,000 + SO2500 0.26 +0.7 2,626,064 + SO2500 0.39 +0.6 2,627,101 - -0.2 2,627,134 + +0.3 2,627,210 - SO2501 0.16 +0.4 2,627,217 - SO2501 0.17 -0.8 2,627,315 + SO2501 0.31 +0.4 2,627,315 + SO2501 0.31 +0.0 2,627,315 + SO2501 0.31 +0.7 2,627,323 - SO2501 0.33 +0.8 2,627,450 + SO2501 0.52 +0.5 2,627,520 + SO2501 0.62 -0.5 2,627,520 + SO2501 0.62 -0.6 2,627,528 - SO2501 0.63 -0.1 2,627,530 - SO2501 0.64 -0.1 2,627,590 + SO2501 0.72 -1.6 2,627,598 - SO2501 0.74 +0.5 2,627,646 - SO2501 0.81 -0.3 2,627,674 + SO2501 0.85 -1.5
Or see this region's nucleotide sequence