Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpmB and radC are separated by 121 nucleotides radC and dfp are separated by 122 nucleotides dfp and dut are separated by 0 nucleotides dut and SO4251 are separated by 31 nucleotides
SO4247: rpmB - ribosomal protein L28 (NCBI ptt file), at 4,424,918 to 4,425,154
rpmB
SO4248: radC - DNA repair protein RadC (NCBI ptt file), at 4,425,276 to 4,425,953
radC
SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302
dfp
SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761
dut
SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386
SO4251
Position (kb) 4426
4427
4428 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4425.358 kb on - strand, within radC at 4425.443 kb on + strand, within radC at 4425.478 kb on - strand, within radC at 4425.494 kb on + strand, within radC at 4425.547 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.592 kb on - strand, within radC at 4425.592 kb on - strand, within radC at 4425.592 kb on - strand, within radC at 4425.658 kb on + strand, within radC at 4425.703 kb on + strand, within radC at 4425.711 kb on - strand, within radC at 4425.818 kb on + strand, within radC at 4425.818 kb on + strand, within radC at 4425.826 kb on - strand, within radC at 4425.826 kb on - strand, within radC at 4425.850 kb on - strand, within radC at 4425.907 kb on + strand at 4425.920 kb on + strand at 4425.928 kb on - strand at 4425.934 kb on + strand at 4427.375 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.519 kb on + strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.546 kb on - strand, within dut at 4427.622 kb on + strand, within dut at 4427.665 kb on - strand, within dut at 4427.791 kb on - strand at 4427.795 kb on + strand at 4427.840 kb on - strand at 4427.840 kb on - strand at 4427.863 kb on + strand, within SO4251 at 4427.908 kb on + strand, within SO4251 at 4427.993 kb on + strand, within SO4251 at 4428.036 kb on + strand, within SO4251 at 4428.057 kb on + strand, within SO4251 at 4428.232 kb on - strand, within SO4251 at 4428.252 kb on + strand, within SO4251
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 4,425,358 - radC SO4248 0.12 -1.9 4,425,443 + radC SO4248 0.25 +1.4 4,425,478 - radC SO4248 0.30 +0.0 4,425,494 + radC SO4248 0.32 +0.1 4,425,547 + radC SO4248 0.40 -0.8 4,425,584 + radC SO4248 0.45 +0.4 4,425,584 + radC SO4248 0.45 -1.0 4,425,584 + radC SO4248 0.45 +0.1 4,425,592 - radC SO4248 0.47 -0.3 4,425,592 - radC SO4248 0.47 -1.2 4,425,592 - radC SO4248 0.47 -0.1 4,425,658 + radC SO4248 0.56 +0.7 4,425,703 + radC SO4248 0.63 -0.3 4,425,711 - radC SO4248 0.64 -2.1 4,425,818 + radC SO4248 0.80 +0.2 4,425,818 + radC SO4248 0.80 -0.2 4,425,826 - radC SO4248 0.81 -0.0 4,425,826 - radC SO4248 0.81 -0.5 4,425,850 - radC SO4248 0.85 +0.7 4,425,907 + +0.1 4,425,920 + -0.1 4,425,928 - +0.3 4,425,934 + +0.2 4,427,375 + dut SO4250 0.16 -2.4 4,427,514 + dut SO4250 0.46 -1.7 4,427,514 + dut SO4250 0.46 -1.2 4,427,514 + dut SO4250 0.46 -3.1 4,427,514 + dut SO4250 0.46 -3.8 4,427,514 + dut SO4250 0.46 -0.6 4,427,519 + dut SO4250 0.47 -0.9 4,427,522 - dut SO4250 0.48 -4.8 4,427,522 - dut SO4250 0.48 -2.5 4,427,522 - dut SO4250 0.48 -2.4 4,427,546 - dut SO4250 0.53 -2.6 4,427,622 + dut SO4250 0.69 -0.4 4,427,665 - dut SO4250 0.79 -3.0 4,427,791 - -4.3 4,427,795 + -1.3 4,427,840 - -4.4 4,427,840 - -1.9 4,427,863 + SO4251 0.12 -4.5 4,427,908 + SO4251 0.19 -3.6 4,427,993 + SO4251 0.34 -2.6 4,428,036 + SO4251 0.41 -3.1 4,428,057 + SO4251 0.44 -2.9 4,428,232 - SO4251 0.74 -2.8 4,428,252 + SO4251 0.77 -1.4
Or see this region's nucleotide sequence